A5053384656- Ocular Infections and Treatments
- Ocular Surface and Contact Lens
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Corneal Surgery and Treatments
- Antifungal resistance and susceptibility
- Corneal surgery and disorders
- Inflammasome and immune disorders
- Head and Neck Cancer Studies
- Dermatology and Skin Diseases
- NF-κB Signaling Pathways
- Parasite Biology and Host Interactions
- Proteoglycans and glycosaminoglycans research
- Antimicrobial Peptides and Activities
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Nanocomposite Films for Food Packaging
- Virus-based gene therapy research
- Pediatric health and respiratory diseases
- Angiogenesis and VEGF in Cancer
- Bacterial biofilms and quorum sensing
- Ocular Diseases and Behçet’s Syndrome
- Psoriasis: Treatment and Pathogenesis
- Parasitic Diseases Research and Treatment
- Cancer-related molecular mechanisms research
Beijing University of Chinese Medicine
2022-2025
Yantai Institute of Coastal Zone Research
2022-2025
University of Chinese Academy of Sciences
2022-2025
Case Western Reserve University
2011-2024
MetroHealth
2024
Cleveland Clinic Lerner College of Medicine
2017-2024
MetroHealth Medical Center
2024
Zhejiang University
2022-2023
Chinese Academy of Sciences
2020-2022
Jinzhou Medical University
2022
Abstract The widely used pseudorabies virus (PRV) Bartha-K61 vaccine has played a key role in the eradication of PRV. Since late 2011, however, disease characterized by neurologic symptoms and high number deaths among newborn piglets occurred Bartha-K61–vaccinated pigs on many farms China. Clinical samples from 15 6 provinces were examined. PRV gE gene was detectable PCR all samples, sequence analysis showed that isolates belonged to relatively independent cluster contained 2 amino acid...
Toll-like receptors (TLRs), which recognize microbial products, have an important role in the host innate immune response. The purpose of present study was to determine whether activation these leads development keratitis and assess common adaptor molecule myeloid differentiation factor-88 (MyD88).Corneal epithelium C57BL/6, TLR2(-/-), TLR9(-/-), MyD88(-/-) mice abraded treated with Pam(3)Cys, LPS, or CpG DNA, bind TLR2, -4, -9, respectively, neutrophil recruitment corneal stroma, haze,...
Calprotectin, a heterodimer of S100A8 and S100A9, is an abundant neutrophil protein that possesses antimicrobial activity primarily because its ability to chelate zinc manganese. In the current study, we showed neutrophils from calprotectin-deficient S100A9(-/-) mice have impaired inhibit Aspergillus fumigatus hyphal growth in vitro infected corneas murine model fungal keratitis; however, was restored by injecting recombinant calprotectin. Furthermore, using calprotectin with mutations...
Pseudomonas aeruginosa is a major cause of blindness and visual impairment in the United States worldwide. Using murine model keratitis which abraded corneas are infected with P. parent ΔfliC (aflagellar) strains 19660 PAO1, we found that F4/80(+) macrophages were predominant cell type cornea expressing TLR2, TLR4, TLR5. Depletion dendritic cells using transgenic Mafia mice, Fas ligand selectively activated these cells, resulted diminished cytokine production cellular infiltration to corneal...
Abstract To examine the role of caspase-1 and NLRC4 inflammasome during bacterial infection, C57BL/6, IL-1β−/−, caspase-1−/−, NLRC4−/− mouse corneas were infected with ExoS/T- or ExoU-expressing Pseudomonas aeruginosa. We found that IL-1β was essential for neutrophil recruitment clearance produced by myeloid cells rather than resident cells. In addition, neutrophils to be primary source mature there no significant difference in processing between C57BL/6 caspase-1−/− corneas. cleavage human...
Abstract Fusarium and Aspergillus species of mold are major causes corneal infections in the United States worldwide, resulting severe visual impairment blindness. As there is evidence for T cell responses to these pathogenic fungi infected individuals, we examined role IL-17A (IL-17) IFN-γ murine models fungal keratitis. We found that C57BL/6 mice given intratracheal or s.c. immunization conidia prior infection exhibited enhanced killing lower opacity compared with unimmunized mice....
This multicenter phase III study evaluated the efficacy and safety of lapatinib, an epidermal growth factor receptor/ErbB2 inhibitor, administered concomitantly with chemoradiotherapy as maintenance monotherapy in patients high-risk surgically treated squamous cell carcinoma head neck (SCCHN).Patients resected stage II to IVA SCCHN, a surgical margin ≤ 5 mm and/or extracapsular extension, were randomly assigned (66 Gy total radiation dose cisplatin 100 mg/m(2) per day on days 1, 22, 43) plus...
Pseudomonas aeruginosa is a leading cause of blinding corneal ulcers worldwide. To determine the role type III secretion in pathogenesis P. keratitis, corneas C57BL/6 mice were infected with strain PAO1 or PAK, which expresses ExoS, ExoT, and ExoY, but not ExoU. PAO1- PAK-infected developed severe disease pronounced opacification rapid bacterial growth. In contrast, ΔpscD ΔpscJ mutants that cannot assemble system, lacking translocator proteins, do develop clinical disease, bacteria are...
Purpose.: Fusarium is a major cause of microbial keratitis, and its ability to form biofilms was suggested as contributing factor in recent outbreaks. We investigated the outbreak isolates (F. solani species complex [FSSC] F. oxysporum [FOSC]) vitro vivo, evaluated their antifungal susceptibilities. Methods.: Biofilm formation assessed using our contact lens model vivo murine model. architecture confocal laser scanning microscopy (CLSM). Susceptibility against amphotericin B (AmB),...
Controlling infection-driven inflammation is a major clinical dilemma because of limited therapeutic options and possible adverse effects on microbial clearance. Compounding this difficulty the continued emergence drug-resistant bacteria, where experimental strategies aiming to augment inflammatory responses for enhanced killing are not applicable treatment infections vulnerable organs. As with corneal infections, severe or prolonged jeopardizes transparency, leading devastating vision loss....
The fungal pathogens Fusarium solani and oxysporum cause severe corneal disease in the United States worldwide were causative organisms a recent outbreak of contact lens-associated keratitis. To characterize innate immunity keratitis, we developed murine model which conidia are injected into stroma. Immunocompetent C57BL/6 mice rapidly opacification associated with neutrophil infiltration clearance hyphae. In contrast, was delayed MyD88-/- mice, resulting uncontrolled growth hyphae stroma...
Fusarium solani and F. oxysporum were the causative organisms of 2005/2006 outbreak contact lens-associated fungal keratitis in United States. The present study was an investigation ability grown as a biofilm on silicone hydrogel lenses to induce keratitis.A clinical isolate lotrafilcon A lenses, 2-mm diameter punch placed abraded corneal epithelium either untreated or cyclophosphamide-treated C57BL/6 mice IL-1R1(-/-), MyD88(-/-), TLR2(-/-), TLR4(-/-) mice. After 2 hours, lens removed,...
Neutrophil extracellular trap (NET) formation requires chromatin decondensation prior to nuclear swelling and eventual release of DNA, which occurs together with cytoplasmic anti-microbial proteins. A key mediator is protein deiminase 4 (PAD4), catalyzes histone citrullination. In the current study, we examined role PAD4 NETosis following activation neutrophils by A. fumigatus hyphal extracts or cell wall β-glucan (curdlan), found that both induced NET human murine neutrophils. Also, using...
Deacetylation of chitin by deacetylases (Cda) results in the formation chitosan. Chitosan, a polymer β1,4 linked glucosamine, plays multiple roles function fungal cell wall, including virulence and evasion host immune responses. In this study chitosan putative CDAs wall structure Aspergillus fumigatus were investigated. Low levels found conidial A. fumigatus. Seven CDA genes identified, disrupted phenotype single mutants septuple No alterations levels, changes growth or detected Δcda1-7...
ABSTRACT Toll-like receptors (TLRs) expressed by the corneal epithelium represent a first line of host defense to microbial keratitis. The current study examined role TLR2, TLR4, and TLR9 common adaptor molecule myeloid differentiation factor 88 (MyD88) in Staphylococcus aureus model inflammation. epithelia C57BL/6, TLR2 −/− , TLR4 MyD88 mice were abraded using trephine epithelial brush exposed heat- or UV-inactivated S. clinical strain 8325-4 other isolates. Corneal thickness haze measured...
An important aspect of wound healing is the recruitment neutrophils to site infection or tissue injury. Lumican, an extracellular matrix component belonging small leucine rich proteoglycan (SLRP) family, one major keratan sulfate proteoglycans (KSPGs) within corneal stroma. Increasing evidence indicates that lumican can serve as a regulatory molecule for several cellular processes, including cell proliferation and migration. In present study, we addressed role in process extravasation...
BackgroundPrevious studies by us and other have provided evidence that leukocytes play a critical role in the development of diabetic retinopathy, suggesting possible innate immune system retinopathy. Since MyD88 is convergence point for signaling pathways (including Toll-Like Receptors (TLRs) interleukin-1ß (IL-1ß)), purpose this study was to assess its dependent on abnormalities develop retina white blood cells related MethodsC57BL/6J mice were made with streptozotocin. Chimeric generated...
Abstract Glioblastoma multiforme (GBM) is the most malignant and aggressive glioma with abnormal expression of genes that mediate glycolytic metabolism tumor cell growth. Petunidin‐3‐ O ‐glucoside (Pt3glc) a kind anthocyanin in red grape derived beverages, representing common naturally occurring anthocyanins reduced incidence cancer heart diseases. In this study, whether Pt3glc could effectively regulate glycolysis to inhibit GBM was investigated by using DBTRG‐05MG lines. Notably, displayed...