A5102768087- Adenosine and Purinergic Signaling
- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Lymphatic System and Diseases
- Maternal Mental Health During Pregnancy and Postpartum
- Microbial metabolism and enzyme function
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Fibroblast Growth Factor Research
- Neuroscience of respiration and sleep
- Pancreatic and Hepatic Oncology Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Genetic Neurodegenerative Diseases
- Neonatal Respiratory Health Research
- Angiogenesis and VEGF in Cancer
- Sleep and related disorders
- Pancreatitis Pathology and Treatment
- Tryptophan and brain disorders
- Health Systems, Economic Evaluations, Quality of Life
- Pregnancy and preeclampsia studies
- Respiratory Support and Mechanisms
- Genomics and Rare Diseases
- Cell Adhesion Molecules Research
- Lipid metabolism and disorders
- Genetic Associations and Epidemiology
University of Turku
2003-2021
Faron Pharmaceutical (Finland)
2013-2015
Finnish Institute for Health and Welfare
2009-2015
Research Council of Finland
2015
University of Helsinki
2015
Turku University Hospital
2011
Tampere University Hospital
2011
Tampere University
2011
Exome sequencing studies in complex diseases are challenged by the allelic heterogeneity, large number and modest effect sizes of associated variants on disease risk presence numbers neutral variants, even phenotypically relevant genes. Isolated populations with recent bottlenecks offer advantages for studying rare as they have deleterious that present at higher frequencies well a substantial reduction variation. To explore potential Finnish founder population low-frequency (0.5–5%)...
Abstract Macrophage mannose receptor (MR) participates in pathogen recognition, clearance of endogenous serum glycoproteins, and antigen presentation. MR is also present on lymphatic vessels, where its function unknown. Here we show that migration lymphocytes from the skin into draining lymph nodes through afferent lymphatics reduced MR-deficient mice, while structure vasculature remains normal these animals. Moreover, a tumor model primary tumors grow significantly bigger MR−/− mice than...
Objective This study aimed to investigate the role of cytokines as intermediates in pathway from increased adiposity disease. Methods BMI and circulating levels up 41 were measured individuals three Finnish cohort studies ( n = 8,293). Mendelian randomization (MR) was used assess impact on BMI‐driven risk obesity‐related diseases. Results Observationally, associated with 19 cytokines. For every SD increase BMI, causal effect estimates strongest for hepatocyte growth factor, monocyte...
Clever-1/Stabilin-1 is a scavenger receptor present on lymphatic and sinusoidal endothelium as well subset of type II macrophages. It also induced vasculature at sites inflammation. However, its in vivo function has remained practically unknown this work addresses those aspects. We demonstrate using models that mediates migration T B lymphocytes to the draining lymph nodes identify adhesive epitope molecule responsible for interaction between endothelium. Moreover, we Ab blocking efficiently...
Lyme neuroborreliosis (LNB) is one of the manifestations disease. Although it known that immune reaction LNB patients dominated by Th1 and Th2 responses have elevated numbers B cells in their cerebrospinal fluid (CSF), not all involved inflammation cytokine secretion been characterized. The current diagnostics based on intrathecal production antibodies. In recent years, measurement chemokine CXCL13 concentration from CSF has introduced as a new promising diagnostic tool for to complement...
Objective— Vascular adhesion protein-1 is an endothelial enzyme that regulates leukocyte traffic and contributes to vascular damage in animal models. The relations of soluble (sVAP-1) with cardiovascular risk factors markers subclinical atherosclerosis at a population level have not been studied. Methods Results— We developed new high-throughput method measured sVAP-1 activities serum 2183 persons (The Cardiovascular Risk Young Finns Study). In women, activity correlated indirectly body mass...
New biomarkers are needed to better predict the severity of acute pancreatitis. CD73/ecto-5'-nucleotidase is an enzyme that generates adenosine, which dampens inflammation and improves vascular barrier function in several disease models. CD73 also circulates a soluble form blood. We studied whether levels development organ failure pancreatitis.A prospective cohort study patients with pancreatitis from 2003 2007.Admissions biggest tertiary care hospital Finland.One hundred sixty-one...
Anagrelide (ANA) is a phosphodiesterase 3A (PDE3A) inhibitor, commonly prescribed for essential thrombocythemia. It also functions as molecular glue, inducing complex formation between PDE3A and Schlafen 12. This association either triggers apoptosis or inhibits proliferation in tumor cells, supporting its use cancer therapy. Conventionally administered orally, ANA undergoes rapid metabolism elimination, resulting short drug exposure time at the site of action. Here, we explored...
Escherichia coli amine oxidase (ECAO), encoded by the tynA gene, catalyzes oxidative deamination of aromatic amines into aldehydes through a well-established mechanism, but its exact biological role is unknown. We investigated ECAO screening environmental and human isolates for characterizing tynA-deletion strain using microarray analysis biochemical studies. The presence did not correlate with pathogenicity. In tynA+ strains, enabled bacterial growth in phenylethylamine, resultant H2O2 was...
Circulating levels of growth factors involved in leucocyte production and angiogenesis could be indicative underlying aberrations tissue homeostasis therefore utilized as predictors risk for all-cause cardiovascular disease (CVD) or cancer mortality.Baseline plasma a range were measured two cohorts the population-based FINRISK study (1997 Discovery cohort, N = 8444, aged 25-74; 2002 Replication 2951, 51-74 years) using multiplexed bead array methodology ELISA. Participants followed up by...
Abstract Context: Metabolic inflammation contributes to the development of insulin resistance (IR), but roles different inflammatory and other cytokines in this process remain unclear. Objective: We aimed at analyzing value predicting future IR. Design, Setting, Participants: measured serum concentrations 48 from a nationwide cohort 2200 Finns (the Cardiovascular Risk Young Study), analyzed their role as independent risk factors for IR 4 years later. Main Outcome Measures: used...
CD73, ecto-5′-nucleotidase, is the key enzyme catalyzing conversion of extracellular AMP to adenosine that controls vascular permeability and immunosuppression. Also prostatic acid phosphatase (PAP) possesses ecto-5′-nucleotidase/AMPase activity present in leukocytes. However, its role related immune system unknown. Therefore, we analyzed enzymatic activities leukocyte subtypes CD73 PAP knockouts generated CD73/PAP double knockout mice elucidate contribution immunological parameters....
Abstract Background In this study, Escherichia coli cells producing red fluorescent protein of Discosoma sp. (drFP583 DsRed) were investigated with flow cytometry by using 488 nm excitation. We also studied whether green (GFP) and drFP583 could be detected simultaneously from a single bacterial cell. Methods Plasmids pDsRed pEGFP used for the production enhanced GFP, respectively, in E. MC1061 cells. To produce GFP simultaneously, plasmids pG9R pG19R constructed. These encode tandem fusions...
Background CD73 dephosphorylates adenosine monophosphate to that is an anti-inflammatory molecule inhibiting immune activation and vascular leakage. Therefore, could be interesting mediator both in sepsis acute kidney injury (AKI). We aimed explore the soluble (sCD73) levels their evolution critically ill patients with severe and, second, scrutinize potential association of sCD73 AKI 90-day mortality. Methods This was a post-hoc laboratory analysis prospective, observational FINNAKI study...