Lonicerae japonicae flos (LJF) and caulis (LJC) are derived from different parts of Lonicera japonica Thunb. (Caprifoliaceae), have been used as herbal remedies to treat various diseases for thousands years with confirmed curative effects. However, little attention has paid illustrating the differences in efficacy perspective phytochemistry. In present study, a simultaneous determination 47 bioactive constituents, including 12 organic acids, flavonoids, six iridoids, 13 amino acids four...

Eucommia ulmoides Oilv. (EU), also called Du-zhong, is a classical traditional Chinese medicine. Its bark, leaf, and male flower are all used for medicinal purposes, Eucommiae Cortex (EC), Folium (EF), Flos Male (EFM). In order to study the difference in synthesis accumulation of metabolites different parts EU, reliable method based on ultra-fast liquid chromatography tandem triple quadrupole mass spectrometry (UFLC-QTRAP-MS/MS) was developed simultaneous determination total 21 constituents,...

Licorice (Glycyrrhiza uralensis Fisch) possesses a substantial share of the global markets for its unique sweet flavor and diverse pharmacological compounds. Cultivated licorice is widely distributed in northwest regions China, covered with land broad range salinities. A preliminary study indicated that suitable salt stress significantly increased content bioactive constituents licorice. However, molecular mechanisms underlying influence salinity on accumulation these remain unclear, which...

Abstract Spica Prunellae is an important Chinese herbal medicine. Because of its good curative effect on various diseases, this herb consumed in large quantities clinical applications. The metabolites are known to change under salt stress; however, the difference protein levels between saline and normal conditions unclear. In study, we used proteomics techniques identify differentially expressed proteins different conditions. (iTRAQ) MS/MS was detect statistically significant changes stress...

As one of the major abiotic stresses, salinity stress may affect physiology and biochemical components Apocynum venetum L. To systematically evaluate quality Apocyni Veneti Folium (AVF) from perspective physiological wide variety bioactive response to various concentrations salt stress, this experiment was arranged on basis ultra-fast liquid chromatography tandem triple quadrupole mass spectrometry (UFLC-QTRAP-MS/MS) technology multivariate statistical analysis. Physiological characteristics...

Pseudostellariae Radix (PR) is an important traditional Chinese herbal medicine (TCM) with vast clinical consumption because of its positive effects. However, little attention has been devoted to simultaneous analysis bioactive components for quality control PR based on different harvesting times, growing habitats, and processing methods. In this research, the was evaluated determination multiple combined grey relational (GRA). A reliable method ultra-fast liquid chromatography tandem triple...

Lonicerae Japonicae Flos (LJF) and (LF) belong to different genera of Caprifoliaceae. They have been historically utilised as herbal medicine treat various diseases. However, the comprehensive assessment them still remains a challenge.To develop method ultra-fast liquid chromatography-tandem triple quadrupole mass spectrometry (UFLC-QTRAP-MS/MS) coupled with multivariate statistical analysis for quality evaluation reveal differential components LJF LF.A validated UFLC-QTRAP-MS/MS was...

Salinity stress significantly affects the contents of bioactive constituents in licorice Glycyrrhiza uralensis. To elucidate molecular mechanism underlying difference accumulation these under sodium chloride (NaCl, salt) stress, seedlings were treated with NaCl and then subjected to an integrated transcriptomic metabolite profiling analysis. The analysis results identified 3,664 differentially expressed genes (DEGs) including transcription factor family MYB basic helix-loop-helix (bHLH)....

Abstract Lonicerae Japonicae Flos (LJF) is an important traditional Chinese medicine for the treatment of various ailments and plays a vital role in improving global human health. However, as unable to escape from adversity, quality sessile organisms dramatically affected by salt stress. To systematically explore formation LJF morphology, physiology, bioactive constituents' response multiple levels stress, UFLC-QTRAP-MS/MS multivariate statistical analysis were performed. Lonicera japonica...

Ophiopogonis Radix, also known as Mai-dong in Chinese, was a commonly used traditional Chinese medicine (TCM) and functional health food. Two products of Radix are largely produced the Sichuan Zhejiang province, which called "Chuan maidong (CMD)" "Zhe (ZMD)" respectively. To distinguish evaluate quality CMD ZMD, an analytical method based on ultra-fast performance liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry (UFLC-QTRAP-MS/MS) established for...

Spatholobi Caulis (SC), the vine stem of Spatholobus suberectus Dunn, is a widely used traditional Chinese medicine (TCM) for treatment blood stasis syndrome and related diseases. Xylem phloem are main structures SC color xylem in red brown or while with resin secretions reddish to dark brown. They alternately arranged plurality concentric eccentric rings. In order investigate distribution patterns metabolites SC, an analytical method based on UFLC-QTRAP-MS/MS was established simultaneous...

Scrophulariae Radix is one of the most popular traditional Chinese medicines (TCMs), which needs to be processed by 'sweating' methods. Primary processing an important link closely relates quality products in this TCM. To facilitate selection suitable method for Radix, study different methods was evaluated based on simultaneous determination multiple bioactive constituents combined with grey relational analysis. The contents iridoid glycosides, phenylpropanoid and organic acids were...

Schisandrae Chinensis Fructus, also called wuweizi in China, was a widely used folk medicine Korea, and Russia. Due to the limited natural resources huge demand of wuweizi, people tend cultivate protect this species. However, quality wild cultivated herbs same species may change. Little attention has been paid comparing based on simultaneous determination its active components, such as lignans organic acids. An analytical method UFLC-QTRAP-MS/MS for 15 including 11 (schisandrin, gomisin D,...

Abstract Introduction Ginseng Flos (GF), the flower bud of Panax ginseng , is a worthy functional food with medicinal potential. A few studies have focused on comprehensive and systematic analysis its major bioactive constituents. Objective The aims are to develop methods ultra‐fast liquid chromatography coupled triple quadrupole‐time flight tandem mass spectrometry (UFLC‐Triple TOF‐MS/MS) quadrupole‐linear ion trap (UFLC‐QTRAP‐MS/MS) for qualitative quantitative saponins in GF. Methodology...

Salt stress affects the metabolic homeostasis of medicinal plants. However, plants are sessile organisms that cannot escape from salt stress. They acclimatize themselves to by reprogramming their pathways. Lonicerae Japonicae Flos (LJF) with strong antioxidant activity is commonly used in traditional Chinese medicine, tea, and beverage. Nevertheless, variation integrated metabolites LJF under different stresses remains unclear. In this study, High Performance Liquid Chromatography tandem...

Scrophulariae Radix is one of the most popular traditional Chinese medicines. The harvesting time closely related to quality products in this medicine. goal study analyze dynamic changes metabolite accumulation Radix. difference constituents harvested at different times was analyzed by liquid chromatography with triple quadrupole-time flight tandem mass spectrometry coupled principal component analysis and partial least-square discriminant analysis. According accurate molecular product ions...

Apocyni Veneti Folium (AVF) is a kind of staple traditional Chinese medicine with vast clinical consumption because its positive effects. However, due to the habitats and adulterants, quality uneven. To control this medicinal herb, in study, AVF was evaluated based on simultaneous determination multiple bioactive constituents combined multivariate statistical analysis. A reliable method ultra-fast liquid chromatography tandem triple quadrupole mass spectrometry (UFLC-QTRAP-MS/MS) developed...

Licorice is one of the ancient and most frequently applied herbs for its diverse phytochemicals. At present, wild resources licorice have rapidly declined with increasing demand proportion cultivated products in market quickly growing. However, different level chemical composition between may result discrepancy quality pharmacological activity. Therefore, an ultra-fast liquid chromatography coupled triple quadrupole-time flight tandem mass spectrometry (UFLC-Triple TOF-MS/MS) method combined...