A5059338299- Organ Transplantation Techniques and Outcomes
- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Liver Disease and Transplantation
- Hepatitis B Virus Studies
- Autophagy in Disease and Therapy
- Tissue Engineering and Regenerative Medicine
- Hepatocellular Carcinoma Treatment and Prognosis
- Organ Donation and Transplantation
- Medical Image Segmentation Techniques
- Renal Transplantation Outcomes and Treatments
- Innovations in Medical Education
- Immune Response and Inflammation
- Advanced Glycation End Products research
- Electrospun Nanofibers in Biomedical Applications
- Pancreatic function and diabetes
- AI in cancer detection
- Pediatric Hepatobiliary Diseases and Treatments
- Cell Image Analysis Techniques
- Elasticity and Material Modeling
- Hepatitis Viruses Studies and Epidemiology
- Drug Transport and Resistance Mechanisms
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Diet and metabolism studies
Jena University Hospital
2016-2025
Friedrich Schiller University Jena
2013-2024
Klinikum Magdeburg
2012-2023
Universitätsklinikum Würzburg
2023
University of Duisburg-Essen
2002-2022
Erlanger Health System
2014-2017
University of Zurich
2017
Bellingham Technical College
2016
Essen University Hospital
2004-2014
Center for Clinical Studies
2013
Histological alterations often constitute a fingerprint of toxicity and diseases. The extent to which these are cause or consequence compromised organ function, the underlying mechanisms involved is matter intensive research. In particular, liver disease associated with altered tissue microarchitecture, in turn may compromise perfusion functionality. Research this field requires development orchestration new techniques into standardized processing pipelines that can be used reproducibly...
Cholestasis is a common complication in liver diseases that triggers proliferative response of the biliary tree. Bile duct ligation (BDL) frequently used model cholestasis rodents. To determine which changes occur three‐dimensional (3D) architecture interlobular bile during cholestasis, we 3D confocal imaging, surface reconstructions, and automated image quantification covering period up to 28 days after BDL. We show highly reproducible sequence remodeling, where cholangiocyte proliferation...
It is well known that isolation and cultivation of primary hepatocytes cause major gene expression alterations. In the present genome-wide, time-resolved study cultivated human mouse hepatocytes, we made observation changes in culture strongly resemble alterations liver diseases. Hepatocytes both species were collagen sandwich monolayer conditions. Genome-wide data also obtained from NAFLD, cirrhosis, HCC hepatitis B virus-infected tissue as livers after partial hepatectomy, CCl4...
Abstract Objective To establish an arterial spin labeling (ASL) protocol for rat livers that improves data reliability and reproducibility perfusion quantification. Methods This study used respiratory-gated, single-slice, FAIR-based ASL imaging with multiple inversion times (TI) in livers. Quality assurance measures included: (1) introduction of mechanical ventilation to ensure consistent respiratory cycles by controlling the rate (45 bpm), tidal volume (10 ml/kg), inspiration: expiration...
Based on the 3-dimensional visualization of vascular supply and drainage, a vessel-oriented resection technique was optimized. The new surgical used to determine maximal reduction in liver mass enabling 50% 1-week survival rate.Determination minimal is necessary clinical as well experimental surgery. In rats, seems depend applied. Extended hepatectomy with removal 90% long regarded lethal model. Introduction approach enabled long-term this model.The lobar anatomy rat livers visualized by...
The anti-malaria drug chloroquine is well known as autophagy inhibitor. Chloroquine has also been used anti-inflammatory drugs to treat inflammatory diseases. We hypothesized that could have a dual effect in liver ischemia/reperfusion (I/R) injury: on the one hand protect against I/R injury via inhibition of response, but other aggravate through autophagy. Rats (n=6 per group) were pre-treated with (60 mg/kg, i.p.) 1 h before warm ischemia, and they continuously subjected daily injection for...
The identification of disease-associated modules based on protein-protein interaction networks (PPINs) and gene expression data has provided new insights into the mechanistic nature diverse diseases. However, their is hampered by detection protein communities within large-scale, whole-genome PPINs. A presented successful strategy detects a PPIN's community structure maximal clique enumeration problem (MCE), which non-deterministic polynomial time-hard problem. This renders approach...
We previously demonstrated that baicalein could protect against liver ischemia/reperfusion (I/R) injury in mice. The exact mechanism of remains poorly understood. Autophagy plays an important role protecting I/R injury. This study was designed to determine whether via induction autophagy rats. Baicalein intraperitoneally injected 1 h before warm ischemia. Pretreatment with prior insult significantly blunted I/R-induced elevations serum aminotransferase levels and improved the histological...
Ischemic preconditioning exerts a protective effect in hepatic ischemia/reperfusion injury. The exact mechanism of ischemic action remains largely unknown. Recent studies suggest that autophagy plays an important role protecting against However, the preconditioning-afforded protection and its regulatory mechanisms liver injury remain poorly understood. This study was designed to determine whether could protect via heme oxygenase-1-mediated autophagy.Laboratory investigation.University animal...
Abstract To overcome the problem of in‐stent restenosis, concept local delivery antiproliferative or immunosuppressive drugs has been introduced into interventional cardiology. Local drug can be achieved by drug‐eluting stents coated with polymer surfaces used for controlled release. However, several coatings have shown an induction inflammatory response and increased neointima formation. In present study, effect a new inorganic ceramic nanoporous aluminum oxide (Al 2 O 3 ) coating on...
Spontaneous orthotopic liver allograft acceptance associated with microchimerism in mice induces tolerance to subsequent skin or heart transplants from the donor but not third-party animals. Despite vivo hyporesponsiveness, vitro MLC and CTL assays showed continuing antidonor reactivity. Cells isolated recipients' spleens grafted livers, when tested assays, were reactive out 3 months same degree as splenocytes obtained either naive presensitized (with heart) mice. Nevertheless, passive...
This study compared measurement of urinary ethyl glucuronide (EtG), a conjugated minor ethanol metabolite with longer detection window than itself, breath alcohol testing and self-report as ways to disclose recent drinking by 18 liver transplant candidates an alcoholic disease diagnosis that underwent addiction group therapy program. At each session, patients were questioned about any consumption in the intervening time, they also performed mandatory test, while observed urine samples for...
Summary Recent studies showing the therapeutic effect of young blood on aging‐associated deterioration organs point to as solution for clinical problems related old age. Given that defective autophagy has been implicated in aging and organ injuries, this study was designed determine aging‐induced alterations hepatic function underlying mechanisms, with a focus autophagy. Aged rats (22 months) were treated pooled plasma (1 ml, intravenously) collected from (3 or aged three times per week 4...
High mobility group box 1 (HMGB1) is a nuclear transcription factor. Once HMGB1 released by damaged cells or activated immune cells, it acts as danger molecule and triggers the inflammatory signaling cascade. Currently, evidence accumulating that posttranslational modifications such oxidation may modulate pro-inflammatory potential of signals. We hypothesized reduce its could take place during prolonged ischemia upon reperfusion. Liver grafts were cold preserved for 24 h flushed with saline...
Liver dysfunction has been known to occur frequently in cases of sepsis. Excessive inflammation and apoptosis are pathological features acute liver failure. Recent studies suggest that activation glycogen synthase kinase- (GSK-) 3 β is involved apoptosis. We aimed investigate the protective effects GSK-3 inhibition on polymicrobial sepsis-induced injury explore possible mechanisms. Polymicrobial sepsis was induced by cecal ligation puncture (CLP), SB216763 used inhibit C57BL/6 mice....