A5084336730- Cell death mechanisms and regulation
- Glioma Diagnosis and Treatment
- Mitochondrial Function and Pathology
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- Prostate Cancer Treatment and Research
- Protein Degradation and Inhibitors
- Advanced biosensing and bioanalysis techniques
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Cancer Research and Treatments
- interferon and immune responses
- Advanced Proteomics Techniques and Applications
- Autophagy in Disease and Therapy
- 3D Printing in Biomedical Research
- Metal complexes synthesis and properties
- Monoclonal and Polyclonal Antibodies Research
- Cancer Cells and Metastasis
- COVID-19 Clinical Research Studies
- Neurogenesis and neuroplasticity mechanisms
- Glycosylation and Glycoproteins Research
- Pluripotent Stem Cells Research
Koç University
2016-2025
Biomed Research Institute
2023
Harvard University
2010-2018
Massachusetts General Hospital
2010-2018
Harvard Stem Cell Institute
2018
Laboratory Imaging (Czechia)
2012
Tufts University
2009-2011
Pfizer (United States)
2011
The resistance of glioma cells to a number antitumor agents and the highly invasive nature that escape primary tumor mass are key impediments eradication tumors in patients. In this study, we evaluated therapeutic efficacy novel PI3-kinase/mTOR inhibitor, PI-103, established lines CD133(+) glioma-initiating explored potential combining PI-103 with stem cell-delivered secretable necrosis factor apoptosis-inducing ligand (S-TRAIL) both vitro orthotopic mouse models gliomas. We show inhibits...
The deregulation of the epidermal growth factor receptor (EGFR) has a significant role in progression tumors. Despite development number EGFR-targeting agents that can arrest tumor growth, their success clinic is limited several types, particularly highly malignant glioblastoma multiforme (GBM). In this study, we generated and characterized EGFR-specific nanobodies (ENb) imageable proapoptotic ENb immunoconjugates released from stem cells (SC) to ultimately develop unique EGFR-targeted...
High mortality rates of glioblastoma (GBM) patients are partly attributed to the invasive behavior tumor cells that exhibit extensive infiltration into adjacent brain tissue, leading rapid, inevitable, and therapy-resistant recurrence. In this study, we analyzed transcriptome motile (dispersive) non-motile (core) GBM using an in vitro spheroid dispersal model identified SERPINE1 as a modulator cell dispersal. Genetic or pharmacological inhibition reduced adhesion by regulating cell-substrate...
Abstract The development of taxane resistance remains a major challenge for castration resistant prostate cancer (CR-PCa), despite the effectiveness taxanes in prolonging patient survival. To uncover novel targets, we performed an epigenetic drug screen on (docetaxel and cabazitaxel) CR-PCa cells. We identified BRPF reader proteins, along with several groups (CBP/p300, Menin-MLL, PRMT5 SIRT1) that act as targets effectively reversing mediated by ABCB1. Targeting BRPFs specifically resulted...
3D platforms are important for monitoring tumor progression and screening drug candidates to eradicate tumors such as glioblastoma multiforme (GBM), a malignant type of human brain tumor. Here, new strategy is reported that exploits visible-light-induced crosslinking gelatin where the reaction carried out in absence an additional crosslinker. Visible light-induced promotes design cancer microenvironment-mimetic system without compromising cell viability during process crosslinker facilitates...
Enzalutamide is a potent second-generation antiandrogen commonly used to treat hormone-sensitive and castration-resistant prostate cancer (CRPC) patients. While initially effective, the disease almost always develops resistance. Given that many enzalutamide-resistant tumors lack specific somatic mutations, there strong evidence epigenetic factors can cause enzalutamide To explore how resistance arises, we systematically test all modifiers in several models of with custom CRISPR library. From...
Harakiri (HRK) is a BH3-only protein of the Bcl-2 family and regulates apoptosis by interfering with anti-apoptotic Bcl-xL proteins. While its function mainly characterized in nervous system, role tumors ill-defined few studies demonstrating HRK silencing tumors. In this study, we investigated most aggressive primary brain tumor, glioblastoma multiforme (GBM). We showed that differentially expressed among established GBM cell lines overexpression can induce cells at different levels. This...
Abstract Androgen deprivation therapy (ADT) is the standard care for prostate cancer (PCa) patients who fail surgery or radiotherapy. While initially effective, almost always recurs as a more aggressive castration resistant (CRPC). Previous studies have demonstrated that chromatin modifying enzymes can play critical role in conversion to CRPC. However, only handful of these potential pharmacological targets been tested. Therefore, this study, we conducted focused shRNA screen previously...
Small molecule inhibitors have previously been investigated in different studies as possible therapeutics the treatment of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). In current drug repurposing study, we identified leukotriene (D4) receptor antagonist montelukast a novel agent that simultaneously targets two important SARS-CoV-2. We initially demonstrated dual inhibition profile through multiscale molecular modeling studies. Next, characterized its effect on both by vitro...
Glioma, as an aggressive type of cancer, accounts for virtually 80% malignant brain tumors. Despite advances in therapeutic approaches, the long-term survival glioma patients is poor (it usually fatal within 12-14 months). Glioma-on-chip platforms, with continuous perfusion, mimic vivo metabolic functions cancer cells analytical purposes. This offers unprecedented opportunity understanding underlying reasons that arise glioma, determining most effective radiotherapy approach, testing...
Characterizing clinically relevant brain metastasis models and assessing the therapeutic efficacy in such are fundamental for development of novel therapies metastatic cancers. In this study, we have developed an vivo imageable breast-to-brain mouse model. Using real time imaging subsequent composite fluorescence imaging, show a widespread distribution micro- macro-metastasis different stages progression. We also extravasation tumour cells close association with blood vessels thus mimicking...
Overcoming drug resistance is a major challenge for cancer therapy. Tumor necrosis factor α-related apoptosis-inducing ligand (TRAIL) potent therapeutic as an activator of apoptosis, particularly in tumor but not healthy cells. However, its efficacy limited by the cell populations to substance. Here, we have addressed this limitation through development controlled release system, matrix-metalloproteinase (MMP)-sensitive and arg-gly-asp-ser (RGDS) peptide functionalized poly (ethylene-glycol)...
Abstract Glioblastoma Multiforme (GBM) is the most common and aggressive primary brain tumor. Despite recent developments in surgery, chemo- radio-therapy, a currently poor prognosis of GBM patients highlights an urgent need for novel treatment strategies. TRAIL (TNF Related Apoptosis Inducing Ligand) potent anti-cancer agent that can induce apoptosis selectively cancer cells. cells frequently develop resistance to which renders clinical application therapeutics inefficient. In this study,...
Abstract Glioma is one of the most malignant types cancer and gliomas remain incurable. One hallmarks glioma its invasiveness. Furthermore, cells tend to readily detach from primary tumor travel through brain tissue, making complete resection impossible in many cases. To expand knowledge regarding invasive behavior glioma, evaluate drug resistance, recapitulate microenvironment, various modeling strategies were proposed last decade, including three-dimensional (3D) biomimetic scaffold-free...
Abstract Non‐infectious virus‐like particles (VLPs) are excellent structures for development of many biomedical applications such as drug delivery systems, vaccine production platforms, and detection techniques infectious diseases including SARS‐CoV‐2 VLPs. The characterization biochemical biophysical properties purified VLPs is crucial methods therapeutics. presence spike (S) protein in their structure especially important since S induces immunological response. In this study, a rapid,...
Abstract We developed surface proteome signatures (SPS) for identification of new biomarkers playing a role in cancer drug resistance. SPS compares antigen expression different cell lines by immunocytochemistry phage display antibody library directed to antigens HT1080 fibrosarcoma cells. applied compare the proteomes two epithelial derived lines, MCF7 and NCI/ADR-RES, which is resistant because overexpression P-glycoprotein (P-gp) efflux pump. Surface proteomic profiling identified CD44 as...
Abstract Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can selectively kill tumor cells. TRAIL resistance in cancers is associated with aberrant expression of the key components apoptotic program. However, how these are regulated at epigenetic level not understood. In this study, we investigated novel mechanisms regulating response glioblastoma multiforme (GBM) cells by a short-hairpin RNA loss-of-function screen. We interrogated 48 genes DNA and histone modification...