A5013662995- Bee Products Chemical Analysis
- Essential Oils and Antimicrobial Activity
- Phytochemistry Medicinal Plant Applications
- Healthcare and Venom Research
- Insect and Pesticide Research
- Venomous Animal Envenomation and Studies
- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Ion channel regulation and function
- Advanced Glycation End Products research
- Botanical Research and Applications
- Agricultural and Food Sciences
- Skin Protection and Aging
- Pomegranate: compositions and health benefits
- Marine Invertebrate Physiology and Ecology
- Inflammatory mediators and NSAID effects
- Entomological Studies and Ecology
- Research on Leishmaniasis Studies
- Exercise and Physiological Responses
- Metabolomics and Mass Spectrometry Studies
- Bladder and Urothelial Cancer Treatments
- Toxin Mechanisms and Immunotoxins
- Growth and nutrition in plants
- Transgenic Plants and Applications
- Biological Control of Invasive Species
Instituto Butantan
2019-2024
Natura (Brazil)
2016
Universidade Estadual Paulista (Unesp)
2007-2015
University of Coimbra
2013
Fundação para o Desenvolvimento da UNESP
2012
Propolis is a bee product and its immunomodulatory action has been the subject of intense investigation lately. The recent discovery characterization family Toll-like receptors (TLR) have triggered great deal interest in field innate immunity due to their crucial role microbial recognition development adaptive immune response. This work aimed evaluate propolis's effect on TLR-2 TLR-4 expression production pro-inflammatory cytokines (IL-1beta IL-6). Male BALB/c mice were treated with propolis...
The aim of this work was to evaluate the antiviral activities Baccharis dracunculifolia (extract and essential oil), propolis some isolated compounds (caffeic cinnamic acids) against poliovirus type 1 (PV1) replication in HEp-2 cells.Three different protocols (pre-, simultaneous post-treatments) were used verify effect addition time variables on PV1 by crystal violet method relative viral RNA quantification real-time PCR for analysing which step virus could interfere.Data revealed that B....
Propolis is a beehive product and its immunomodulatory action has been documented; however, little known concerning mechanisms of on human cells. influence the initial events immune response was assessed, evaluating cell markers, cytokine production fungicidal activity monocytes.Toll-like receptor (TLR)-2, TLR-4, leukocyte antigen-DR cluster differentiation (CD)80 expression by monocytes assessed using FACSCalibur flow cytometer, (tumour necrosis factor (TNF)-α interleukin (IL)-10)...
Propolis is a sticky dark-colored material showing very complex chemical composition that honeybees collect from plants. It has been used in folk medicine since ancient times, due to several biological properties, such as antimicrobial, anti-inflammatory, antioxidant and immunomodulatory activities, among others. Its antitumor action vivo vitro also reported, using propolis extracts or its isolated compounds. The goal of this work was evaluate propolis's cytotoxic on human laryngeal...
Propolis is a beehive product used in traditional medicine due to its biological properties. It shows complex chemical composition including phenolics, such as cinnamic acid (Ci). The mechanisms of action propolis have been the subject research recently; however, involvement Ci on activity was not investigated immune cells. effects were evaluated human monocytes, assessing expression Toll-like receptors (TLRs), HLA-DR, and CD80. Cytokine production (TNF- α IL-10) fungicidal monocytes well....
Aim: Geopropolis is produced by stingless bees from resinous materials of plants, adding salivary secretions, wax, mud or clay, and has been used in folk medicine for the treatment respiratory diseases dermatoses.Therefore, it important to study its antibacterial, antitumor immunomodulatory properties are confirm ethnopharmacological applications.This work aimed evaluate chemical composition, antimicrobial, anti-tumoral activities.Materials Methods: composition was investigated using GC-MS...
Baccharis dracunculifolia is the most important vegetal source of propolis in southeast Brazil, and researchers have been investigating its biological properties. Propolis a complex resinous hive product collected by bees from several plants, showing very chemical composition. It has employed since ancient times due to therapeutic properties, such as antimicrobial, anti-inflammatory, antioxidant, immunomodulatory antitumour activities, among others. The goal this work was compare cytotoxic...
Geopropolis is produced by indigenous stingless bees from the resinous material of plants, adding soil or clay. Its biological properties have not been investigated, such as propolis, and herein its cytotoxic action on canine osteosarcoma (OSA) cells was evaluated. OSA a primary bone neoplasm diagnosed in dogs being an excellent model vivo to study human OSA. spOS-2 cultures were isolated tumor dog with incubated geopropolis, 70% ethanol (geopropolis solvent), carboplatin after 6, 24, 48, 72...
Abstract Objectives Researchers have been interested in investigating the mechanisms of action propolis and compounds involved its biological activity; however, effect isolated constituents on human immune cells still deserves investigation. Thus, this study aimed to verify caffeic acid monocytes an attempt effects innate immunity, analyse participation activity. Methods Monocytes viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method after incubation...
Abstract Objectives Propolis has been used in folk medicine different regions of the world including Latin America. is a resinous mixture substances collected by honey bees from several botanical sources, and its composition contains rich chemical variety, depending on geographical area plant sources. Our aim was to compare modulatory effect propolis samples three countries America (Brazil, Cuba Mexico) pro- anti-inflammatory cytokine production (tumor necrosis factor (TNF)-α interleukin...
Propolis is a beehive product and its immunomodulatory action has been well documented; however, little known concerning activity on the immune system of stressed mice. This work investigated possible role propolis against immunosuppressive effects induced by stress in mice, assessing pro-inflammatory cytokine (IL-1β IL-6) production Toll-like receptor (TLR-2 TLR-4) expression spleen cells. BALB/c mice were divided into 3 groups: G1 was considered control; G2 submitted to restraint for days,...
Increased collagen-derived advanced glycation end-products (AGEs) are consistently related to painful diseases, including osteoarthritis, diabetic neuropathy, and neurodegenerative disorders. We have recently developed a model combining two-dimensional glycated extracellular matrix (ECM-GC) primary dorsal root ganglion (DRG) that mimicked pro-nociceptive microenvironment. However, culturing cells is still challenge for large-scale screening studies. Here, we characterized new using ECM-GC as...
Abstract Objectives Mucocutaneous leishmaniasis is associated with a strong Th1 immune response to Leishmania, which modulates chemokines and their receptors expression, affecting migratory capacity. There are no antileishmanial vaccines available chemotherapy still relies on the potentially toxic pentavalent antimonials. Propolis bee product immunomodulatory antiparasite activities, researchers have been attracted its potential for development of new drugs. This work investigated effects...
Pain is a worldwide public health problem and its treatment still challenge since clinically available drugs do not completely reverse chronic painful states or induce undesirable effects. Crotalphine 14 amino acids synthetic peptide that induces potent long-lasting analgesic effect on acute pain models, peripherally mediated by the endogenous release of dynorphin A desensitization transient receptor potential ankyrin 1 (TRPA1) receptor. However, effects crotalphine central nervous system...
Osteosarcoma (OSA) is a primary bone neoplasm frequently diagnosed in dogs. The biology of OSA pet dogs identical to that pediatric patients, and it has been considered an excellent model vivo study human OSA. Since the individual response chemotherapy unpredictable considering propolis natural product with several biological properties, this work evaluated cytotoxic action on canine cells. cell culture was obtained from tumor dog Cell viability assessed after incubation propolis, 70%...
Neurodegenerative diseases are one of the major causes death worldwide, characterized by neurite atrophy, neuron apoptosis, and synapse loss. No effective treatment has been indicated for such so far, search new drugs is being increased in last years. Animal venoms' secretion/venom can be an alternative discovery molecules, which could prototype a treatment. Here, we present biochemical characterization activity extract from box jellyfish
Crotoxin (CTX), the main neurotoxin from Crotalus durissus terrificus snake venom, has anti-inflammatory, immunomodulatory and antinociceptive activities. However, CTX-induced toxicity may compromise its use. Under this scenario, use of nanoparticle such as nanostructured mesoporous silica (SBA-15) a carrier might become feasible approach to improve CTX safety. Here, we determined benefits SBA-15 on CTX-related neuroinflammatory properties during experimental autoimmune encephalomyelitis...
Most anti-inflammatory drugs currently adopted to treat chronic inflammatory joint diseases can alleviate symptoms but they do not lead remission. Therefore, new and more efficient are needed block the course of diseases. Animal venoms, rich in bioactive compounds, contribute as valuable tools this field research. In study, we first demonstrate direct action venoms on cells that constitute articular joints. We established a platform consisting cell-based assays evaluate release cytokines...