A5010979091- Epigenetics and DNA Methylation
- Neurotransmitter Receptor Influence on Behavior
- Genetic Associations and Epidemiology
- Genetic Syndromes and Imprinting
- Tryptophan and brain disorders
- Genetic Mapping and Diversity in Plants and Animals
- Phosphodiesterase function and regulation
- Cell Image Analysis Techniques
- Birth, Development, and Health
- Receptor Mechanisms and Signaling
- Diet and metabolism studies
- Autism Spectrum Disorder Research
- RNA Research and Splicing
- Hippo pathway signaling and YAP/TAZ
- Glioma Diagnosis and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Personality Disorders and Psychopathology
- Adipose Tissue and Metabolism
- Computational Drug Discovery Methods
- Microtubule and mitosis dynamics
- Identity, Memory, and Therapy
- Neonatal Respiratory Health Research
- Single-cell and spatial transcriptomics
- Cholinesterase and Neurodegenerative Diseases
Central Institute of Mental Health
2022-2025
Heidelberg University
2022-2025
University Hospital Heidelberg
2022-2025
German Cancer Research Center
2025
Epigenome, transcriptome, and proteome analyses of postmortem brains have revealed initial molecular insights into cocaine use disorder (CUD). However, the inter-relationship between these omics contribution individual cell types remains largely unknown. We present an in-depth analysis changes in ventral striatum CUD at multi-omics single-cell resolution. Integrative microRNA sequencing (microRNA-seq), RNA (RNA-seq), proteomics datasets 41 individuals single-nuclei RNA-seq a subset 16...
Glioblastoma (GBM) progression and therapeutic resistance are significantly influenced by complex interactions between tumor cells the brain microenvironment, particularly neurons. However, studying these in physiologically relevant conditions has remained challenging due to limitations existing model systems. Here, we present hGliCS (human glioma-cortical spheroid), a novel fully human that overcomes key of current approaches combining patient-derived GBM with mature cortical neurons...
Structural and functional changes of the brain are assumed to contribute excessive cocaine intake, craving, relapse in use disorder (CUD). Epigenetic transcriptional were hypothesized as a molecular basis for CUD-associated alterations. Here we performed multi-omics study CUD by integrating epigenome-wide methylomic (N = 42) transcriptomic 25) data from same individuals using postmortem tissue Brodmann Area 9 (BA9). Of N 1 057 differentially expressed genes (p < 0.05), one gene, ZFAND2A, was...
Abstract Structural and functional changes of the brain are assumed to contribute excessive cocaine intake, craving, relapse in use disorder (CUD). Epigenetic transcriptional were hypothesized as a molecular basis for CUD-associated alterations. Here we performed multi-omics study CUD by integrating epigenome-wide methylomic (N=42) transcriptomic (N=25) data from same individuals using postmortem tissue Brodmann Area 9 (BA9). Of N=1,057 differentially expressed genes (p<0.05), one gene,...
<title>Abstract</title> Structural and functional alterations in the brain's reward circuitry are present cocaine use disorder (CocUD), but their molecular underpinnings remain unclear. To investigate these mechanisms, we performed single-nuclei multiome profiling on postmortem caudate nucleus tissue from six individuals with CocUD eight controls. We profiled 31,178 nuclei, identifying 13 cell types including D1- D2-medium spiny neurons (MSNs) glial cells. observed 1,383 differentially...
(1) Background: Epigenome-wide association studies (EWAS) in peripheral blood have repeatedly found associations between tobacco smoking and aberrant DNA methylation (DNAm), but little is known about DNAm signatures of the human brain, which may contribute to pathophysiology addictive behavior observed chronic smokers. (2) Methods: We investigated similarity matched postmortem brain samples (
Abstract Epigenome, transcriptome, and proteome analyses of postmortem brains have revealed initial molecular insights into cocaine use disorder (CUD). However, the inter-relationship between these –omics contribution individual cell types remain largely unknown. We present an in-depth analysis changes in ventral striatum CUD at multi-omics single-cell resolution. Integrative microRNA-seq, RNA-seq, proteomics datasets 41 individuals single-nuclei RNA-seq a subset 16 conserved deregulation...
Abstract Environmental and genetic risk factors contribute to the development of borderline personality disorder (BPD). We conducted largest GWAS BPD date, meta-analyzing data from 12,339 cases 1,041,717 controls European ancestry, identified six independent associated genomic loci, nine genes in gene-based analysis. observed a single-nucleotide polymorphism (SNP) heritability 17.3% derived polygenic scores (PGS) predicted 4.6% phenotypic variance case-control status. showed strongest...
Abstract Lissencephaly is a developmental cortical malformation characterized by reduced to absent gyri and disorganized cortex, often leading severe impairments in affected individuals life expectancy. Heterozygous mutations the LIS1 gene, encoding regulator of microtubule motor dynein, cause lissencephaly with different clinical severities. While disease spectrum correlates degree lissencephaly, location type mutation may not. We leveraged forebrain-type organoids from LIS1-lissencephaly...