A5084509186- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Receptor Mechanisms and Signaling
- Immune Response and Inflammation
- Cancer Immunotherapy and Biomarkers
- bioluminescence and chemiluminescence research
- Asthma and respiratory diseases
- Chemokine receptors and signaling
- Neuropeptides and Animal Physiology
- Peroxisome Proliferator-Activated Receptors
- Adenosine and Purinergic Signaling
- Sphingolipid Metabolism and Signaling
- Advanced Fluorescence Microscopy Techniques
- Monoclonal and Polyclonal Antibodies Research
- Retinoids in leukemia and cellular processes
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Escherichia coli research studies
- Molecular Communication and Nanonetworks
- Protein Kinase Regulation and GTPase Signaling
- Lymphatic System and Diseases
- Innovative Microfluidic and Catalytic Techniques Innovation
- Digestive system and related health
- Antimicrobial Peptides and Activities
Lund University
2012-2022
Skåne University Hospital
2021
Tokyo Institute of Technology
2006
Wallenberg Wood Science Center
2001-2003
CD103+CD11b+ dendritic cells (DCs) represent the major migratory DC population within small intestinal lamina propria (SI-LP), but their in vivo function remains unclear. Here we demonstrate that survival was dependent on interferon regulatory factor 4 (IRF4). Mice with a deletion Irf4 displayed reduced numbers of interleukin 17 (IL-17)-secreting helper T (Th17) and failed to support Th17 cell differentiation draining mesenteric lymph nodes (MLN) following immunization. The latter associated...
Here, the ligand binding, activation, and tissue distribution of orphan G protein-coupled receptor (GPCR) GPR92 were studied. binds is activated by compounds based on lysophosphatidic acid (LPA) backbone. The binding LPA to was high affinity (<i>K</i><sub>D</sub> = 6.4 ± 0.9 nM) led an increase in both phosphoinositide hydrolysis cAMP production. atypical that it has a low sequence homology with classic LPA<sub>1-3</sub> receptors (21-22%). Expression mainly found heart, placenta, spleen,...
The identification of intestinal macrophages (mφs) and dendritic cells (DCs) is a matter intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature origins CD103(-) MPs remain controversial. We show here that CD103(-)CD11b(+) can separated clearly into DCs mφs based on phenotype, gene profile, kinetics. CD64(-)CD103(-)CD11b(+) are classical being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C(hi) monocytes. Surprisingly,...
Small intestinal lamina propria (SI-LP) CD103(+) dendritic cells (DCs) are imprinted with an ability to metabolize vitamin A (retinol), a property underlying their enhanced capacity induce the gut-homing receptors CC chemokine receptor-9 and α4β7 on responding T cells. In this study, we demonstrate that imprinting of DCs is itself critically dependent occurs locally within small intestine (SI). The major metabolite retinoic acid (RA) induced retinol-metabolizing activity in both vitro vivo,...
The adult immune system consists of cells that emerged at various times during ontogeny. We aimed to define the relationship between developmental origin and composition B cell pool unperturbed hematopoiesis. Lineage tracing stratified murine based on timing output, revealing a substantial portion originated within restricted neonatal window. In addition B-1a cells, early-life time-stamped included clonally interrelated IgA plasma in gut bone marrow. These were actively maintained by memory...
Conventional dendritic cells (cDCs) consist of two major functionally and phenotypically distinct subsets, cDC1 cDC2, whose development is dependent on sets transcription factors. Interferon regulatory factor 8 (IRF8) required at multiple stages development, but its role in committed remains unclear. Here, we used Xcr1-cre to delete Irf8 demonstrate that for maintaining the identity cDC1. In absence Irf8, acquired transcriptional, functional, chromatin accessibility properties cDC2. This...
Despite the key role of primary and secondary lymphoid organ stroma in immunity, our understanding heterogeneity ontogeny these cells remains limited. Here, we identify a functionally distinct subset BP3−PDPN+PDGFRβ+/α+CD34+ stromal adventitial both lymph nodes (LNs) thymus that is located within vascular niche surrounding PDPN−PDGFRβ+/α−Esam-1+ITGA7+ pericytes. CD34+ developed late embryonic postnatal LNs originated, along with pericytes, from common anlage-seeding progenitor population....
The CC chemokine receptor (CCR)9 is expressed on the majority of small intestinal, but few colonic, T cells, whereas its ligand CCL25 constitutively by intestinal epithelial cells. As such, CCR9/CCL25 have been proposed to play a central role in regulating not colonic immune responses and thus organize regionalized immunity within mucosa. Here, we demonstrate that at reduced levels cells distal compared with proximal intestine, which correlated less efficient CCR9-dependent effector...
Abstract The vitamin A metabolite and transcriptional modulator retinoic acid (RA) is recognized as an important regulator of epithelial cell homeostasis in several tissues. Despite the known importance compartment thymus T development selection, potential role RA regulation thymic cortical medullary has yet to be addressed. In this study, using fetal organ cultures, we demonstrate that endogenous signaling promotes (TEC) cell-cycle exit restricts TEC cellularity preferentially compartment....
Intestinal cDC1 drive the generation of FoxP3 + CD8 T regs to maintain tolerance epithelial-derived self-antigen.
Abstract The chemokine CCL25 is selectively and constitutively expressed in the small intestinal epithelium plays an important role mediating lymphocyte recruitment to this site. In study, we demonstrate that expression murine epithelial cells independent of signaling through lymphotoxin β receptor not enhanced by inflammatory stimuli, pathways involved driving most other chemokines. We define a transcriptional start site gene region −141 −5 proximal exon 1 required for minimal promoter...
Despite the essential role of thymic epithelial cells (TEC) in T cell development, signals regulating TEC differentiation and homeostasis remain incompletely understood. In this study, we show a key vivo for vitamin A metabolite, retinoic acid (RA), homeostasis. absence RA signaling TEC, cortical (cTEC) CD80loMHC class IIlo medullary displayed subset-specific alterations gene expression, which cTEC included genes involved proliferation, differentiation. Mice whose were unable to respond...
A microfluidic system for long-term real-time monitoring of dynamic cellular events immobilized human cells was investigated. The luciferase reporter gene activity in the cell line HFF11, based on HeLa cells, used as model system. were silicon flow-through microchips and continuously supplied with a medium at 2 μL/min while maintaining chip 37 °C. HFF11 designed high-throughput screening ligands seven-transmembrane receptors. When ligand binds, receptor is activated cascade intracellular...
Initiation of adaptive immunity to particulate antigens in lymph nodes largely depends on their presentation by migratory dendritic cells (DCs). DC subsets differ capacity induce specific types immunity, allowing subset-specific DC-targeting influence vaccination and therapy outcomes. Faithful drug design, however, requires exact understanding versus global activation mechanisms. cDC1, the subset DCs that excel supporting toward viruses, intracellular bacteria, tumors, express uniquely high...
The small intestinal lamina propria contains large numbers of IFNγ-producing T helper (Th1) cells that play important roles in homeostasis and host defense, but the mechanisms underlying their development remain poorly understood. Here, we demonstrate Th1 accumulate SI-LP after weaning are maintained there long term. While both Th17 cell accumulation was microbiota dependent, uniquely required IL-27 MHCII expression by cDC1. This reflected a requirement for signaling priming rather than...