A5074626446- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Chromatin Remodeling and Cancer
- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- Protein Degradation and Inhibitors
- Genomics and Phylogenetic Studies
- Ubiquitin and proteasome pathways
- Renal and related cancers
- Down syndrome and intellectual disability research
- RNA modifications and cancer
- DNA Repair Mechanisms
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- Developmental Biology and Gene Regulation
- Cancer Mechanisms and Therapy
- Histone Deacetylase Inhibitors Research
- Plant Pathogens and Fungal Diseases
- Retinal Development and Disorders
- 14-3-3 protein interactions
- Congenital heart defects research
- Neurogenesis and neuroplasticity mechanisms
- melanin and skin pigmentation
Centre for Genomic Regulation
2015-2025
Universitat Pompeu Fabra
2010-2020
Karolinska Institutet
2011-2018
Centre for Biomedical Network Research on Rare Diseases
2008-2010
Centro de Investigación Biomédica en Red
2010
Instituto de Salud Carlos III
2008
Instituto de Investigación de Enfermedades Raras
2008
Barcelona Biomedical Research Park
2007
Duran i Reynals Hospital
2003
Epithelial-to-mesenchymal transition (EMT) is fundamental to both embryogenesis and tumor metastasis. The Notch intercellular signaling pathway regulates cell fate determination throughout metazoan evolution, overexpression of activating alleles oncogenic in mammals. Here we demonstrate that activity promotes EMT during cardiac development transformation via transcriptional induction the Snail repressor, a potent evolutionarily conserved mediator many tissues types. In embryonic heart,...
The Polycomb group (PcG) of proteins defines a subset factors that physically associate and function to maintain the positional identity cells from embryo adult stages. PcG has long been considered paradigmatic model for epigenetic maintenance gene transcription programs. Despite intensive research efforts unveil molecular mechanisms action proteins, several fundamental questions remain unresolved: How many different complexes exist in mammalian cells? are targeted specific loci? does...
The cellular origin and molecular mechanisms regulating pigmentation of head neck are largely unknown. Melanocyte specification is controlled by the transcriptional activity Mitf, but no general logic has emerged to explain how Mitf progenitor activities consolidate melanocyte cell fates. We show that cranial melanocytes arise from at least two different sources: initially nerve-associated Schwann precursors (SCPs) later a source independent nerves. Unlike midbrain-hindbrain cluster which...
Profiling the chromatin-bound proteome (chromatome) in a simple, direct, and reliable manner might be key to uncovering role of yet uncharacterized chromatin factors physiology disease. Here, we have designed an experimental strategy survey chromatome proliferating cells by using DNA-mediated pull-down (Dm-ChP) technology. Our approach provides global view cellular under normal physiological conditions enables identification proteins de novo. Integrating Dm-ChP with genomic functional data,...
Dual-specificity tyrosine-phosphorylated and regulated kinase (DYRK) proteins are an evolutionarily conserved family of protein kinases, with members identified from yeast to humans, that participate in a variety cellular processes. DYRKs serine/threonine kinases activated by autophosphorylation on tyrosine residue the activation loop. The member DYRK1A has been shown phosphorylate several cytosolic number splicing transcription factors, including nuclear factor T cells family. In present...
Chromocenters are established after the 2-cell (2C) stage during mouse embryonic development, but factors that mediate chromocenter formation remain largely unknown. To identify regulators of 2C heterochromatin establishment, we generated an inducible system to convert stem cells (ESCs) 2C-like cells. This conversion is marked by a global reorganization and dispersion H3K9me3-heterochromatin foci, which then reversibly formed upon re-entry into pluripotency. By profiling chromatin-bound...
Chromocenters are established after the 2-cell (2C) stage during mouse embryonic development, but factors that mediate chromocenter formation remain largely unknown. To identify regulators of 2C heterochromatin establishment in mice, we generated an inducible system to convert stem cells (ESCs) 2C-like cells. This conversion is marked by a global reorganization and dispersion H3K9me3-heterochromatin foci, which then reversibly formed upon re-entry into pluripotency. By profiling...
Raf-MEK-extracellular signal-regulated kinase (Erk) signaling initiated by growth factor-engaged receptor tyrosine kinases (RTKs) is modulated an intricate network of positive and negative feedback loops which determine the specificity spatiotemporal characteristics intracellular signal. Well-known antagonists RTK are Sprouty proteins. The activity proteins phosphorylation. However, little known about responsible for these posttranslational modifications. We identify DYRK1A as one protein...
Pluripotency of embryonic stem cells (ESCs) is maintained by transcriptional activities and chromatin modifying complexes highly organized within the chromatin. Although much effort has been focused on identifying genome-binding sites, little known their dynamic association with across cell divisions. Here, we used a modified version iPOND (isolation proteins at nascent DNA) technology to identify large protein network enriched DNA in ESCs. This comprehensive unbiased proteomic...
In vertebrates, GATA2 is a master regulator of hematopoiesis and expressed throughout embryo development in adult life. Although the essential role mouse well established, its involvement during early human hematopoietic not clear. By combining time-controlled overexpression with genetic knockout experiments, we found that GATA2, at mesoderm specification stage, promotes generation hemogenic endothelial progenitors their further differentiation to progenitor cells, negatively regulates...
Embryo size, specification, and homeostasis are regulated by a complex gene regulatory signaling network. Here we used expression signatures of Wnt-activated mouse embryonic stem cell (mESC) clones to reverse engineer an mESC We identify NKX1-2 as novel master regulator preimplantation embryo development. find that Nkx1-2 inhibition reduces nascent RNA synthesis, downregulates genes controlling ribosome biogenesis, translation, transport, induces severe alteration nucleolus structure,...
Embryonic stem cells (ESCs) display an abbreviated cell cycle, resulting in a short doubling time and rapid proliferation. The histone variant H2A.X is critical for proliferation of cells, although mechanistic insights have remained obscure. Here, we show that defines the rate mouse ESC independently DNA damage response pathway, it associates with three major chromatin-modifying complexes. Our functional biochemical analyses demonstrate H2A.X-associated factors mediate H2A.X-dependent effect...