A5063521333- Advanced MRI Techniques and Applications
- Protein Structure and Dynamics
- Neurological disorders and treatments
- Functional Brain Connectivity Studies
- Neuroscience and Neuropharmacology Research
- Hearing, Cochlea, Tinnitus, Genetics
- Advanced NMR Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Alzheimer's disease research and treatments
- Bacteriophages and microbial interactions
- Rheumatoid Arthritis Research and Therapies
- Advanced Neuroimaging Techniques and Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Enzyme Structure and Function
- Radiopharmaceutical Chemistry and Applications
- Acute Ischemic Stroke Management
- NMR spectroscopy and applications
- Vestibular and auditory disorders
- Animal Genetics and Reproduction
- Drug Transport and Resistance Mechanisms
- Nerve injury and regeneration
- Bacterial Genetics and Biotechnology
- Molecular spectroscopy and chirality
- Chemical Synthesis and Analysis
- Nanoparticle-Based Drug Delivery
Karolinska University Hospital
2012-2025
Karolinska Institutet
2014-2024
American College of Rheumatology
2023
Science for Life Laboratory
2015
Stockholm University
2000-2010
Tallinn University of Technology
2010
University of California, San Diego
2009
Howard Hughes Medical Institute
2009
Central nervous system injuries are accompanied by scar formation. It has been difficult to delineate the precise role of scar, as it is made several different cell types, which may limit damage but also inhibit axonal regrowth. We show that scarring neural stem cell-derived astrocytes required restrict secondary enlargement lesion and further loss after spinal cord injury. Moreover, progeny exerts a neurotrophic effect for survival neurons adjacent lesion. One distinct component glial...
Abstract PFG‐NMR methods were used to measure the translational diffusion coefficients for Aβ peptide involved in Alzheimer's disease and also a series of fragments this peptide. The peptides ranged from pentamer full length Aβ(1–40). They studied at 25° C physiological pH aqueous solution. measured coefficients, including those known monomeric peptides, fitted without systematic deviations scaling law function molecular mass. We concluded that under these conditions Aβ(1–40) is form. From...
The temperature‐induced structural transitions of the full length Alzheimer amyloid β‐peptide [Aβ(1–40) peptide] and fragments it were studied using CD 1 H NMR spectroscopy. peptide undergoes an overall transition from a state with prominent population left‐handed 3 (polyproline II; PII)‐helix at 0 °C to random coil 60 °C, average ΔH 6.8 ± 1.4 kJ·mol −1 per residue, obtained by fitting Zimm–Bragg model data. is noncooperative for shortest N‐terminal fragment Aβ(1–9) weakly cooperative...
Failure of axonal regeneration in the central nervous system (CNS) is mainly attributed to a lack intrinsic neuronal growth programs and an inhibitory environment from glial scar. Phosphatase tensin homolog (PTEN) major negative regulator and, as such, inhibiting its activity has been considered therapeutic target for spinal cord (SC) injuries (SCIs). Using novel model rat cervical avulsion, we show that treatment with retinoic acid receptor β (RARβ) agonist results locomotor sensory...
The Alzheimer peptide fragment Aβ(12−28) was studied at millimolar concentration by parallel experiments with high-resolution nuclear magnetic resonance (NMR) and circular dichroism (CD) in solution a pH close to the isoelectric point of peptide. A preparation procedure using low temperature ionic strength buffer gave sample stable reproducible properties. Reversible changes secondary structure state aggregation were variation temperature. High-temperature promotes β-sheet induction, whereas...
Rodent models are developed to enhance understanding of the underlying biology different brain disorders. However, before interpreting findings from animal in a translational aspect understand human disease, fundamental step is first have knowledge similarities and differences biological systems studied. In this study, we analyzed verified four known networks termed: default mode network, motor dorsal basal ganglia ventral network using resting state functional MRI (rsfMRI) humans rats. Our...
Abstract A tissue-engineered oesophageal scaffold could be very useful for the treatment of pediatric and adult patients with benign or malignant diseases such as carcinomas, trauma congenital malformations. Here we decellularize rat oesophagi inside a perfusion bioreactor to create biocompatible biological scaffolds that mimic native architecture, resist mechanical stress induce angiogenesis. Seeded allogeneic mesenchymal stromal cells spontaneously differentiate (proven by gene-, protein...
Significance A common feature of all domestic animals is their tame behavior and lack fear for humans. Consistent with this, we have previously demonstrated that genes a role in brain or neural development been particularly targeted during rabbit domestication. Here show, using high-resolution magnetic resonance imaging, rabbits an altered architecture consistent reduced emotional processing, including attention to behaviorally relevant stimulation, such as detection, learning, expression,...
The biarsenical−tetracysteine motif is a useful tag for genetic labeling of proteins with small molecules in living cells. present study concerns the structure 12 amino acid peptide FLNCCPGCCMEP bound to fluorophore ReAsH based on resorufin. 1H NMR spectroscopy was used determine solution complex formed between and moiety. Structure calculations results showed that backbone fairly well defined, hairpinlike turn, similar type-II β-turn, by central CPGC segment. most stable when As2 bonded C4...
<b><i>Background:</i></b> Neuroinflammation triggered by infection or trauma is the cause of central nervous system dysfunction. High-mobility group box 1 protein (HMGB1), released from stressed and dying brain cells, a potent neuroinflammatory mediator. The proinflammatory functions HMGB1 are tightly regulated post-translational redox modifications, we here investigated detailed responses induced individual isoforms. <b><i>Methods:</i></b>...
The appearance of anti-citrullinated protein antibodies (ACPAs) in the circulation represents a major risk factor for developing rheumatoid arthritis (RA). Patient-derived ACPAs have been shown to induce pain and bone erosion mice, suggesting an active role pathogenicity RA. We undertook this study investigate whether can tenosynovitis, early sign RA, addition loss these symptoms are dependent on peptidyl arginine deiminase 4 (PAD4).Monoclonal generated from plasma cells RA patients were...
The trans-vessel wall device (TW-device) is a new endovascular tool for precise and safe delivery of various payloads (cells, viral, modified RNA, chemotherapy, growth factors) in oncology regenerative medicine. twofold aim this study was to assess cell engraftment tumor using the TW-device transplantation evaluate its ability directly access solid tumors. We used VX2 model rabbit kidney compare percutaneously implanted fresh cells with injections cryopreserved cells. demonstrated...
The interactions of Alzheimer's amyloid β-peptide with cyclodextrins were studied by 1H NMR: the translational diffusion coefficient peptide and chemical shift changes presence variable concentrations cyclodextrins. For full-length peptide, Aβ(1−40), combined results are consistent a model where aromatic side chains interact β-cyclodextrin dissociation constants in millimolar range. data two molecules bound per peptide. binding occurs at sites, F19 and/or F20 Y10, KdF = 4.7 mM KdY 6.6 mM,...
The conformational space available to the flexible molecule α-D-Manp-(1→2)-α-D-Manp-OMe, a model for α-(1→2)-linked mannose disaccharide in N- or O-linked glycoproteins, is determined using experimental data and molecular simulation combined with maximum entropy approach that leads converged population distribution utilizing different input information. A database survey of Protein Data Bank where structures having constituent were retrieved resulted an ensemble >200 structures. Subsequent...
Eight fluorinated nanoparticles (NPs) are synthesized, loaded with doxorubicin (DOX), and evaluated as theranostic delivery platforms to breast cancer cells. The multifunctional NPs formed by self‐assembly of either linear or star‐shaped amphiphilic block copolymers, segments incorporated in the hydrophilic corona carrier. sizes confirm that small circular formed. release kinetics data particles reveals clear hydrophobic core dependence, longer sustained from larger cores, suggesting DOX...
Hutchinson–Gilford progeria syndrome (HGPS) is a segmental progeroid with multiple features suggestive of premature accelerated aging. Accumulation progerin thought to underlie the pathophysiology HGPS. However, despite ubiquitous expression lamin A in all differentiated cells, HGPS mutation results organ-specific defects. For example, bone and skin are strongly affected by HGPS, while brain appears be unaffected. There no definite explanations as variable sensitivity disease among different...
We show for the first time that secondary structure of Alzheimer β‐peptide is in a temperature‐dependent equilibrium between an extended left‐handed 3 1 helix and flexible random coil conformation. Circular dichroism spectra, recorded at 0.03 mM peptide concentration, shifted towards increasing lower temperatures. High resolution nuclear magnetic resonance (NMR) spectroscopy has been used to study fragment Aβ(12–28) aqueous solution 0°C higher NMR translation diffusion measurements observed...
The solution structure of the porcine gastrointestinal peptide hormone motilin was determined in presence sodium dodecyl sulfate (SDS) micelles at 28 °C using 1H nuclear magnetic resonance, full relaxation matrix analysis, and calculations based on restrained molecular dynamics. SDS is described by a reverse γ-turn β-turn type II N terminal end, an α-helical region middle molecule, extended C terminus. position molecule relative to probed adding spin-labeled stearic acids, containing...
Background and Purpose— Ischemic stroke has been shown to cause hypermetabolism of glucose in the ischemic penumbra. Experimental clinical data indicate that infarct-related systemic hyperglycemia is a potential therapeutic target acute stroke. However, studies aiming for control have neither improved functional outcome nor reduced mortality. Thus, further on metabolism brain are warranted. Methods— We used rat model preserves collateral flow. The animals were analyzed by [2- 18...
Background: Hypoxia following traumatic brain injury (TBI) is a severe insult shown to exacerbate the pathophysiology, resulting in worse outcome. The aim of this study was investigate effects hypoxic focal TBI model by monitoring edema, lesion volume, serum biomarker levels, immune cell infiltration, as well expression hypoxia-inducible factor 1-alpha (HIF-1α and vascular endothelial growth (VEGF). Material methods: Female Sprague-Dawley rats (n=73, including sham naïve) were used....