A5067507983- Hearing, Cochlea, Tinnitus, Genetics
- Connexins and lens biology
- Hearing Loss and Rehabilitation
- Neuroscience of respiration and sleep
- Cancer-related molecular mechanisms research
- Vestibular and auditory disorders
- Autophagy in Disease and Therapy
- Single-cell and spatial transcriptomics
- Photovoltaic System Optimization Techniques
- Fibroblast Growth Factor Research
- Silicon Carbide Semiconductor Technologies
- Thyroid Disorders and Treatments
- Ear Surgery and Otitis Media
- Electromagnetic wave absorption materials
- RNA regulation and disease
- interferon and immune responses
- Photoreceptor and optogenetics research
- Plant responses to water stress
- Tactile and Sensory Interactions
- Congenital Ear and Nasal Anomalies
- Congenital heart defects research
- Nicotinic Acetylcholine Receptors Study
- Ion Channels and Receptors
- Genomics, phytochemicals, and oxidative stress
- Genomic variations and chromosomal abnormalities
Shanghai Jiao Tong University
2019-2025
XinHua Hospital
2017-2025
Anhui University
2022
Adenoid hypertrophy is a common disorder of childhood, and has an unclear pathogenesis. At the beginning COVID-19 pandemic, there was significant reduction in incidence adenoid children under long-term home quarantine, providing rare research model to explore pathogenesis treatment targets adenoidal children. Before during quarantine period, adenoids that underwent surgery were detected using label-free proteomics. Differences protein expression analyzed Gene Ontology, Kyoto Encyclopedia...
Mucopolysaccharidosis type II (MPS II) is an X-linked lysosomal storage disorder caused by pathogenic variants in the IDS gene encoding iduronate-2-sulfatase (IDS), which hydrolyzes sulfate groups dermatan and heparan sulfate. The current treatment for MPS includes enzyme replacement therapy hematopoietic stem cell transplantation (HSCT). Both therapies have shown limited penetration through blood-brain barrier. Anecdotal cases been reported with HSCT benefit to treat neurological problems...
GJB2 and GJB6 are adjacent genes encoding connexin 26 (Cx26) 30 (Cx30), respectively, with overlapping expressions in the inner ear. Both associated commonest monogenic hearing disorder, recessive isolated deafness DFNB1. Cx26 plays an important role auditory development, while of Cx30 remains controversial. Previous studies found that knockout mice had severe loss along a 90% reduction Cx26, another mouse model showed normal nearly half preserved. In this study, we used CRISPR/Cas9...
The Kölliker's organ is a transient epithelial structure during cochlea development that gradually degenerates and disappears at postnatal 12-14 days (P12-14). While apoptosis has been shown to play an essential role in the degeneration of organ, another programmed cell death, autophagy, remains unclear. In our study, autophagy markers including microtubule associated protein light chain 3-II (LC3-II), sequestosome 1 (SQSTM1/p62) Beclin1 were detected supporting cells through...
Mutations in the GJB2 gene that encodes connexin 26 (Cx26) are predominant cause of prelingual hereditary deafness, and most frequently encountered variants complete loss protein function. To investigate how Cx26 deficiency induces we examined levels apoptosis autophagy Gjb2 loxP/loxP ; ROSA26 CreER mice injected with tamoxifen on day birth. After weaning, these exhibited severe hearing impairment reduced expression cochlear duct. Terminal deoxynucleotidyl transferase dUTP nick end labeling...
Age-related hearing loss (ARHL) is the most prevalent sensory disorder in elderly. Currently, no treatment can effectively prevent or reverse ARHL. Aging auditory organs are often accompanied by exacerbated oxidative stress and metabolic deterioration. Here, we report effect of deuterated oxygen (D2O), also known as "heavy water", mouse models Supplementing normal diet with 10% D2O from 4 to 9 weeks age lowered thresholds at selected frequencies treated mice compared untreated control group....
The hair cells of the cochlea play a decisive role in process hearing damage and recovery, yet knowledge their regeneration is still limited. Greater epithelial ridge (GER) cells, type cell present during cochlear development that has characteristics precursor sensory cell, disappear at time maturation development. Its evolution remain mysterious for many years. Here, we performed single-cell RNA sequencing to profile gene expression landscapes rats’ basal membrane from P1, P7, P14...
Ribbon synapses are important structures in transmitting auditory signals from the inner hair cells (IHCs) to their corresponding spiral ganglion neurons (SGNs). Over last few decades, deafness has been primarily attributed deterioration of cochlear rather than ribbon synapses. Hearing dysfunction that cannot be detected by hearing threshold is defined as hidden loss (HHL). The relationship between and FGF22 deletion remains unknown. In this study, we used a 6-week-old knockout mice model...
Background: To reduce surgical complications and avoid lifelong thyroid hormone replacement, hemithyroidectomy is preferred in children adolescents with benign nodular disease. However, hypothyroidism following may occur, postoperative replacement for usually administered without a full understanding of the clinical characteristics hypothyroidism. Methods: investigate incidence risk factors after adolescents, to identify whether necessary, retrospective review 43 patients under 18 years age...
Autophagy and apoptosis have a complex interplay in the early embryo development. The development of spiral ganglion neurons (SGNs) addition to Corti’s organ mammalian cochlea remains crucial first two-week postnatal period. To investigate roles autophagy SGNs, light microscopy was used observe morphological changes SGNs. number SGNs decreased from P1 P7 plateaued P10 P14. Immunohistochemistry results revealed positive expression cleaved-caspase3, bcl-2, microtubule-associated protein chain...
Greater epithelial ridge cells, a transient neonatal cell group in the cochlear duct, which plays crucial role functional maturation of hair cell, structural development tectorial membrane, and refinement audio localization before hearing. cells are methodologically homogeneous, while whether different subtypes existence this intriguing region degeneration mechanism during postnatal poorly understood. In present study, single-cell RNA sequencing was performed on duct rats at day 1 (P1) 7...
Aiming at the switch fault diagnosis of diode neutral point clamped (NPC) three-level inverter, by studying changes circuit working conditions after NPC a and location strategy inverter based on line voltage deviation is proposed. In this strategy, estimated under normal operation obtained from switching state circuit, comparing actual with voltage. By judging value deviation, flag output realized. Then, characteristic law failure different switches, specific faulty switches This method...
Objective: To investigate the hearing protection outcomes of different drug-eluting analog electrode arrays implanted into guinea pig cochleae. Methods: Sixty pigs were randomly divided a negative control group and five experimental groups separately with blank (drug carrier), dexamethasone (DXM), aracytine (Ara-C), Ara-C+DXM, nicotinamide adenine dinucleotide (NAD+) eluting arrays. Micro CT was used to supervise surgical procedure. Auditory brainstem response (ABR) thresholds measured...
Abstract The ribbon synapses of cochlear inner hair cells (IHCs) employ efficient vesicle resupply to enable fast and sustained release rates. However, the molecular mechanisms these physiological activities remain unelucidated. Previous studies showed that RAB‐specific GTPase‐activating protein TBC1D24 controls endosomal trafficking synaptic vesicles (SVs) in Drosophila mammalian neurons, mutations may lead non‐syndromic hearing loss or associated with DOORS syndrome humans. In this study,...
Abstract Adenosine triphosphate (ATP), which plays a crucial role in both developing and mature cochleae, is released from greater epithelial ridge (GER) supporting cells of the rat cochlea, but organelles ATP stored have not yet been identified. Thus, we studied involved storage suggest that lysosomes provide this function. GER newborn rats were isolated, purified, cultured, labeled vesicles within identified via confocal microscopy transmission electron (TEM). release after glycyl‐ L...
Objective: To investigate the effect of autoinflation on prognosis OME in children. Methods: Total 325 pediatric patients, age ranged from 3 to 8 years, with OME(486 ears)diagnosed our department January 2019 2020 were collected. Among them, 177 males, 148 females. Disease course 25 86 days. According watchful waiting and application during follow-up, these children divided into two groups including 183 cases(271 ears) 142 cases(215 ears), respectively. The average auditory threshold...
Kölliker's organ, which is a transient structure of cochlea during development, in late embryonic and early postnatal period, one the signs cochlear immaturity.Kölliker's organ degradates after sensory structures become sensitive to external sound. The putative role important for generating intrinsic spontaneous activity whichpromotes development maturation fully functional auditory...