A5100347245- Barrier Structure and Function Studies
- Neurological Disease Mechanisms and Treatments
- Gut microbiota and health
- Connexins and lens biology
- Cancer-related molecular mechanisms research
- Gastric Cancer Management and Outcomes
- Pancreatic and Hepatic Oncology Research
- Ultrasound and Hyperthermia Applications
- Neurogenesis and neuroplasticity mechanisms
- Renal cell carcinoma treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Peripheral Artery Disease Management
- Lymphoma Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Child and Adolescent Psychosocial and Emotional Development
- Photoacoustic and Ultrasonic Imaging
- Mindfulness and Compassion Interventions
- Sphingolipid Metabolism and Signaling
- Neuroscience of respiration and sleep
- Ferroptosis and cancer prognosis
- Venous Thromboembolism Diagnosis and Management
- Zebrafish Biomedical Research Applications
- Axon Guidance and Neuronal Signaling
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Gastrointestinal Tumor Research and Treatment
Chinese Academy of Medical Sciences & Peking Union Medical College
2022-2024
Qiqihar Medical University
2021-2023
The First Affiliated Hospital, Sun Yat-sen University
2019
Sun Yat-sen University
2019
Lu'an First People's Hospital
2017
University of Manitoba
2016
China Medical University
2013-2014
Capital Medical University
2014
Henry Ford Health System
2001-2006
We investigated the hypothesis that endothelial cells activated by erythropoietin (EPO) promote migration of neuroblasts. This is based on observations in vivo treatment focal cerebral ischemia with EPO enhances neuroblasts to ischemic boundary, a site containing and angiogenic microvasculature. To model microenvironment within boundary zone, we used coculture system mouse brain (MBECs) neural progenitor derived from subventricular zone adult mouse. Treatment MBECs recombinant human (rhEPO)...
The adult rodent brain is capable of generating neuronal progenitor cells in the subventricular zone, and dentate gyrus hippocampus, throughout life animal. Signals that regulate cell proliferation, differentiation, migration are not well known. We report administration a nitric oxide donor, (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl) aminio]diazen-1-ium-1,2-diolate (DETA/NONOate), to young rats significantly increases proliferation zone gyrus. Treatment with DETA/ NONOate also neurogenesis...
The present study aimed to investigate the effects of alcohol on intestinal epithelial barrier permeability and expression tight junction‑associated proteins, zonula occludens‑1 (ZO‑1) claudin‑1. An alcohol‑treated Caco‑2 cell monolayer in vitro model was used whether is able induce dysfunction decrease ZO‑1 MTT assay results revealed unaltered viability at concentrations <5%. cells 5% groups exhibited a significant time‑dependent transepithelial electrical resistance (TEER) an increase...
Treating the vascular elements within neurovascular unit is essential for protecting and repairing brain after stroke. Acute injury on endothelial systems results in disruption of blood-brain barrier (BBB), while post-ischemic angiogenesis plays an important role delayed functional recovery. Here, we considered alterations microvessel integrity to be targets recovery, tested natural compound morroniside as a therapeutic approach restore injured tissue rat model focal cerebral ischemia....
Microvessel endothelial cells form part of the blood-brain barrier, a restrictively permeable interface that allows transport only specific compounds into brain. Cardiolipin is mitochondrial phospholipid required for function electron chain and ATP generation. We examined role cardiolipin in maintaining necessary to support barrier properties brain microvessel cells. Knockdown terminal enzyme synthesis, synthase, hCMEC/D3 resulted decreased cellular levels compared controls. The reduction...
Noncardiogenic lung edema is a key factor affecting the prognosis of acute injury (ALI). Previous studies have been focused on regulatory roles ceramide vascular endothelial barrier functions and had already identified as mediator involved in formation edema. However, effects epithelial were still unknown. This study aimed to investigate function alveolar cells. Primary mouse type II cells (AECII) grown Transwell polyester membranes construct monolayer, stimulated with different...
In this work, we investigated the potential role of small G protein RhoA in ethanol-induced tight junction (TJ) disassembly and increased intestinal epithelial barrier (IEB) permeability. Our study used Caco-2 cells as an vitro IEB model short hairpin RNA (shRNA) interference to establish whether plays a TJ opening. shRNA partially inhibited leakage restored normal transepithelial electrical resistance (TEER) values IEB. Moreover, prevented shift occludin distribution from insoluble soluble...
Abstract Background The combination of anti‐PD‐1 antibody serplulimab and chemotherapy is considered standard first‐line therapy for advanced esophageal squamous cell carcinoma (ESCC), but few later‐line treatments are available. Here we evaluated the therapeutic efficacy recombinant, humanized anti‐EGFR HLX07 when used alone or together with against ESCC. Methods This open‐label, non‐randomized, two‐cohort, phase 2 trial involved patients 18‐75 years old histologically cytologically...
The present study aimed to investigate the effect of Y-27632, an inhibitor Rho-associated protein kinase (ROCK), which belongs a family downstream effectors activated RhoA, on ethanol-induced increase in permeability intestinal epithelial barrier (IEB). vitro model IEB was established usiung Caco-2 cells, and cells were pretreated with Y-27632 prior treatment ethanol for 60 min. Transepithelial resistance (TEER) paracellular marker Lucifer yellow flux measurements performed assess...
798 Background: Claudin18.2 (CLDN18.2) is specifically expressed in the tight junction of gastric epithelial cells, and has been identified as a promising target pancreatic cancer well several other malignancies. MIL93 humanized afucosylated IgG1 monoclonal antibody targeting CLDN18.2 with enhanced ADCC function, which demonstrated superior activity compared zolbetuximab vitro vivo, respectively. We conducted multicenter, dose escalation expansion phase 1 study for advanced solid tumors....
Venous ulcer is a common contributor to chronic venous insufficiency (CVI) of lower limbs, which seriously affects the life quality patients. In this study, we researched expression characteristics microRNA-301a-3p (miR-301a-3p) in patients with CVI and investigated impact miR-301a-3p on dysfunction human umbilical vein endothelial cells (HUVECs). The plasma level normal controls, varicose great saphenous vein, limbs were measured. We adopted Interleukin-1β (IL-1β), H2O2, oxygen glucose...
This study aimed to explore the expression level of miR-539 in blood-brain barrier permeability induced by cerebrovascular occlusion and its mediated mechanism. Altogether, 48 patients with cerebral vascular lesions from March 2018 June 2020 were collected. The peripheral blood serum subjects was analyzed qRT-PCR, participants divided into two groups according results head neck ultrasound CTA hemodynamics. MCAO model ischemia established rats, detected qRT-PCR brain tissues different rats....
Clear cell renal carcinoma (ccRCC) is one of the cancers that posed a severe threat to human life on global scale. The ADAMTS family has been proven be involved in number tumor types, although it yet unknown how they relate ccRCC. mRNA expression matrix and other clinically relevant information 607 ccRCC were sourced from TCGA database. role genes was determined by differential gene analysis set enrichment (GSEA). Employing stage grading, mutation, survival analysis, most linked prognosis...
This study evaluated pembrolizumab-conjugated, doxorubicin (DOX)-loaded microbubbles (PDMs) in combination with ultrasound (US) as molecular imaging agents for early diagnosis of B cell lymphomas, and a targeted drug delivery system. Pembrolizumab, monoclonal CD20 antibody, was attached to the surfaces DOX-loaded microbubbles. PDM binding lymphoma cells assessed using immunofluorescence. The cytotoxic effects PDMs (PDMs + US) were vitro CD20+ CD20– lines, its antitumor activities Raji...
3086 Background: Claudin18.2 (CLDN18.2) is specifically expressed in the tight junction of gastric epithelial cells, and has been identified as a promising target pancreatic cancer well several other malignancies. MIL93 humanized IgG1 monoclonal antibody targeting CLDN18.2. From January 2021, we started multicenter, dose escalation expansion phase 1 study MIL93, for treatment patients with advanced solid tumors (Protocol number MIL93-CT101, Beijing Mabworks Biotech Co. Ltd.) at 6 clinical...