A5020931124- Mesenchymal stem cell research
- Body Contouring and Surgery
- dental development and anomalies
- Tissue Engineering and Regenerative Medicine
- Wound Healing and Treatments
- Nerve injury and regeneration
- Bone Metabolism and Diseases
- Periodontal Regeneration and Treatments
- Electrospun Nanofibers in Biomedical Applications
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Adipose Tissue and Metabolism
- Bone and Dental Protein Studies
- Lymphatic System and Diseases
- Cancer Cells and Metastasis
- TGF-β signaling in diseases
- 3D Printing in Biomedical Research
- Nerve Injury and Rehabilitation
- Oral and Maxillofacial Pathology
- Bacterial biofilms and quorum sensing
- Neurogenesis and neuroplasticity mechanisms
- Bone Tissue Engineering Materials
- Hedgehog Signaling Pathway Studies
- Plant Water Relations and Carbon Dynamics
- Dermatologic Treatments and Research
University Hospital Regensburg
2014-2024
University of Regensburg
2009-2023
Caritas Hospital
2021
Regensburg University of Applied Sciences
2020
Wound repair is a quiescent mechanism to restore barriers in multicellular organisms upon injury. In chronic wounds, however, this program prematurely stalls. It known that patterns of extracellular signals within the wound fluid are crucial healing. Extracellular pH (pHe) precisely regulated and potentially important signaling wounds due its diverse cellular effects. Additionally, sufficient oxygenation prerequisite for cell proliferation protein synthesis during tissue repair. was,...
Human dental follicle cells (DFCs) are progenitor cells. Recent studies supposed that osteogenic differentiation of DFCs is controlled by growth factors such as BMP2 and IGF2, but their influence on the has not been investigated in detail. We examined after induction with BMP2, IGF2 a standard medium (ODM) dexamethasone. The alkaline phosphatase (ALP) activity calcium accumulation demonstrated all treatments, most effective ODM. Interestingly, markers process osteoblast were much higher...
The transcription factor DLX3 plays a decisive role in bone development of vertebrates. In neural-crest derived stem cells from the dental follicle (DFCs), is differentially expressed during osteogenic differentiation, while other factors such as DLX5 or RUNX2 are not highly induced. has therefore differentiation DFCs, but its actual biological effects and regulation unknown. This study investigated DLX3-regulated processes DFCs. After overexpression, DFCs acquired spindle-like cell shape...
Conduits preseeded with either Schwann cells or stem differentiated into demonstrated promising results for the outcome of nerve regeneration in defects.The concept this trial combines repair by means a commercially available guidance conduit and preseeding autologous, undifferentiated, adipose tissue-derived cells. Adipose were harvested from rats subsequently seeded onto U.S. Food Drug Administration-approved type I collagen conduit. Sciatic gaps 10 mm length created, was performed...
Lipofilling is an option for breast reconstruction after tumor resection to avoid the complications of implant-based reconstruction. Although some concerns exist regarding oncological safety tissue rich in mesenchymal stem cells with their proangiogenic and proliferation-supportive properties, there are also reports that adipose-tissue-derived can exhibit antitumoral properties. We isolated primary cells. Both conditioned medium exosomes were harvested from cell culture used treat cancer...
Background: Dental follicle cells (DFCs) are neural crest cell–derived and the genuine precursor of cementoblast alveolar osteoblasts. After osteogenic differentiation, expression levels transcription factor zinc BTB domain containing 16 ( ZBTB16 ) were significantly increased. is associated with process differentiation in bone marrow–derived mesenchymal stem crucial for runt‐related 2 RUNX2 ). It proposed that plays also a role DFCs into Methods: In this study, by alkaline phosphatase (ALP)...
To evaluate whether and how the transcription factor early growth response gene 1 (EGR1) affects osteogenic differentiation of dental stem cells.Dental cells from apical papilla (SCAPs) follicle (DFCs) were transfected with EGR1-specific siRNA or EGR-1 expression plasmid. Gene regulation was verified at protein level by Western blotting. The factors distal-less homeobox 3 (DLX3), alkaline phosphatase (ALP) bone morphogenetic 2 (BMP2), which are all regulators markers in cells, determined...
Lipofilling is a popular technique to treat volume loss in aging patients. The isolated adipose tissue composed of adipocytes and stromal vascular fraction cells, which include adipose-derived stem cells (ASC). We hypothesize that the patient's wrinkle severity scale (WSS) satisfaction on global aesthetic improvement (GAIS) can be improved after using concentrated lipoaspirate. Fourteen patients (54 years ± 11.09 years) with midface area underwent waterjet-assisted liposuction (Human Med AG,...
Background: Multiple symmetric lipomatosis (MSL) is defined as a disorder of nonencapsulated adipose tissue growth. Its prevalence indicated 1:25,000 and affects, stated in the literature, mainly Mediterranean males (male:female ratio 15:1). Phenotypes are still classified by Donhauser 1991. We report clinical phenotypic data largest patient cohort investigated Germany so far. Methods: Forty-five patients diagnosed with MSL at University Hospital Regensburg between 2007 2017 were...
To date, reliable data to support the general use of biodegradable materials for bridging nerve defects are still scarce. We present outcome regeneration following type I collagen conduit repair in patients with large-diameter gaps. Ten underwent using a conduit. Patients were re-examined at minimal follow-up 14.0 months and mean 19.9 months. Regeneration tissue within conduits was assessed by conduction velocity (NCV), static two-point discrimination (S2PD) test, as disability arm shoulder...
In plastic surgery, lipofilling is a frequent procedure. Unsatisfactory vascularization and impaired cell vitality can lead to unpredictable take rates in the fat graft. The proliferation neovascularization inducing properties of adipose tissue-derived stem cells may contribute solve this problem. Therefore, enrichment grafts with studied intensively. However, it difficult compare these studies because many factors-often not precisely described-are influencing results. Our study summarizes...
Abstract Little is known on the causes and pathogenesis of adipose tissue disorder (familial) Multiple Symmetric Lipomatosis (MSL). In a four-generation MSL-family, we performed whole exome sequencing (WES) in 3 affected individuals 1 obligate carrier identified Calcyphosine-like ( CAPSL ) as most promising candidate gene for this family. Screening 21 independent patients excluded coding sequence variants common monogenic cause, but using immunohistochemistry found that was down-regulated...
Background: The cause of the rare fat distribution disorder multiple symmetric lipomatosis is unknown. Independent reports suggest a higher proliferative activity, hormone resistance, and involvement mitochondrial function in disease. Methods: authors performed morphologic comparison affected unaffected tissues five unrelated patients generated adipose-derived stem cell cultures from tissue samples characterized them as possible cellular model evolution. investigated activity expression...
Platelet-rich plasma is a current subject of studies on chronic wound healing therapy due to possible pro-angiogenic effects. Microvascular compromise represents the major component in radiogenic complications. The effects platelet-rich irradiated cells cutaneous process are poorly understood so far. In this study, interaction endothelial and adipose-derived stem conjunction with treatment investigated context radiation Therefore, expression surface-marker CD90 CD31 was determined. Moreover,...
Background: Lipofilling is one of the most often performed surgical procedures in plastic and reconstructive surgery. Lipoaspirates provide a ready source stem cells secreted factors that contribute to neoangiogenesis fat graft survival. However, regulations about enrichment these beneficial are ambiguous. In this study, authors tested whether combination centrifugation homogenization allowed viable lipoaspirates through selective removal tumescent solution, blood, released lipids without...
Lipedema is a chronic condition characterized by disproportionate and symmetrical enlargement of adipose tissue, predominantly affecting the lower limbs women. This study investigated use metabolomics in lipedema research, with objective identifying complex metabolic disturbances potential biomarkers for early detection, prognosis, treatment strategies. The group (n = 25) comprised women diagnosed lipedema. controls were 25 lean obese females, both matched age. In patients lipedema, there...
Autologous fat grafts and adipose-derived stem cells (ASCs) can be used to treat soft tissue defects. However, the results are inconsistent sometimes comprise resorption necrosis. This might due insufficient vascularization. Platelet-rich plasma (PRP) is a source of concentrated autologous platelets. The growth factors cytokines released by platelets facilitate angiogenesis. simultaneous use PRP improve regeneration potential grafts. optimal ratio has yet elucidated. A byproduct preparation...
Synthetic nerve conduits have emerged as an alternative to guide axonal regeneration in peripheral gap injuries. Migration of Schwann cells (SC) from stumps has been demonstrated one essential factor for defects. In this experiment, SC viability and migration were investigated various materials determine the optimal conditions regeneration. Cell assays conducted collagen I, laminin, fibronectin, lysine ornithine. The highest values cell detected whereas fibronectin was most stimulatory...