Background Chloroplast genomes supply valuable genetic information for evolutionary and functional studies in plants. The past five years have witnessed a dramatic increase the number of completely sequenced chloroplast with application second-generation sequencing technology plastid genome projects. However, cost-effective high-throughput DNA (cpDNA) extraction becomes major bottleneck restricting application, as conventional methods are difficult to make balance between quality yield...

Objective To investigate the effects of ilex kudingcha C. J. Tseng (kuding tea), a traditional beverage in China, on metabolic disorders C57BL/6 mice induced by high-fat diets. Design For preventive experiment, female were fed with standard diet (Chow), (HF), and mixed 0.05% ethanol extract kuding tea (EK) for 5 weeks. therapeutic 3 months, then split EK was given oral gavages 2 weeks at 50 mg/day/kg. Body weight daily food intake amounts measured. At end treatment, adipocyte images assayed...

Rheum palmatum has been used most frequently in the weight-reducing formulae traditional Chinese medicine. However, components of that play antiobesity role are still uncertain. Here, we tested effect two major compounds on db/db mouse. We found rhein (100 mg kg(-1) day(-1)), but not emodin, reduced fat weight Using diet-induced obese (DIO) C57BL/6 mice, identified blocked high-fat obesity, decreased mass and size white brown adipocytes, lowered serum cholesterol, LDL fasting blood glucose...

AbstractFour anthocyanins, namely petunidin-3,5-O-diglucosides(L1), petuinidi-3-O-rutinoside(trans-p-coumaroyl)-5-O-glucosides(L2),cyanidin-3-O-(trans-p-coumaroyl)-diglucoside(N1), and pelargonidin -3-O-(trans-coumaroyl)-diglucoside (N2) have been obtained by preparative HPLC from Lycium ruthenicum Murray Nitraria Tangutorum Bobr. The stability antioxidant capacity of these four individual anthocyanins were carried out in this paper. For examination, pH (pH 1, 3, 5, 7, 9, 11 13),...

Abstract Background The genus Caragana encompasses multiple plant species that possess medicinal and ecological value. However, some of are quite similar in morphology, so identifying this based on their morphological characteristics is considerably complex. In our research, illumina paired-end sequencing was employed to investigate the genetic organization structure tibetica turkestanica , including previously published chloroplast genome sequence 7 plants. Results lengths C. genomes were...

The primary objective of this study was to explore the prognostic significance serum cholinesterase (CHE) and metabolic parameters obtained from 18 F-fluorodeoxyglucose (FDG) PET/computed tomography (CT) scans in patients with non-small cell lung cancer (NSCLC). A retrospective observational cohort conducted 202 NSCLC patients. Serum CHE evaluated alongside tumor volume (MTV) total lesion glycolysis (TLG) derived PET/CT scans. correlation between these overall survival (OS) analyzed using...

Percutaneous coronary intervention (PCI) can effectively restore myocardial perfusion in patients with ST-segment elevation infarction (STEMI). Nevertheless, STEMI may still experience a "no-reflow" phenomenon after PCI. Accordingly, this study focused on the clinical value of low-dose dobutamine stress contrast echocardiography (MCE) for evaluating microcirculation and long-term prognosis This included 70 receiving Low-dose MCE was performed to detect viable myocardium at 72 h PCI...

The genus Caragana, known for its adaptability and high forage value, is commonly planted to rehabilitate barren land prevent desertification. Several Caragana species are also used medicinal purposes. Analysis of synonymous codon usage bias their primary influencing factors in chloroplast genomes aims provide insights into molecular research germplasm innovation plants. GC content the five ranged from 36.00% 37.10%, showing a preference codons ending A/U, although was weak. screening...

Abstract Background Premature ovarian insufficiency (POI) is a common clinical problem, however, there are currently no effective therapies. Pyroptosis induced by the NLRP3 inflammasome considered possible mechanism of POI. Placental mesenchymal stem cells (PMSCs) have excellent immunomodulatory potential and offer promising method for treating Methods Female Sprague–Dawley rats were randomly divided into four treatment groups: control (no POI), POI with PMSCs, PMSCs transplant, hormones...

Abstract Background Numerous species within the genus Caragana have high ecological and medicinal value. However, identification based on morphological characteristics is quite complicated in genus. To address this issue, we analyzed complete plastid genome data for Results We obtained chloroplast genomes of two species, arborescens opulens , using Illumina sequencing technology, with lengths 129,473 bp 132,815 bp, respectively. The absence inverted repeat sequences indicated that they could...

Jiaotaiwan (JTW), which is composed of Coptis chinensis (CC) and cinnamon (CIN), one the most well-known traditional Chinese medicines. In this study, we investigated antidiabetic effects mechanism JTW in db/db mice. Results showed that significantly decreased level fasting blood glucose improved insulin tolerance better than CC or CIN alone. also effectively protected pancreatic islet shape, augmented activation AMP-activated protein kinase (AMPK) liver, increased expression transporter 4...

The dioecious property of the sea buckthorn (Hippophae rhamnoides L.) prevents sex recognition via traditional observation at juvenile stage, thus impeding breeding and economic cropping; A random amplified polymorphic DNA (RAPD) a sequence characterized region (SCAR) markers were used to identify sexes. total 45 decamer primers screen genomic pools staminate pistillate genotypes for genetic polymorphisms. One female sex-linked marker was identified. D15 (5′-CATCCGTGCT-3′) particular band...

Hippophae rhamnoides L., a fruit which is rich in natural chemical ingredients, has become an important raw material of health food, medicine, and cosmetics. In the present work, method using 2-(5-benzoacridine)ethyl-p-toluenesulfonate as pre-column derivatization reagent with high sensitivity, selectivity, short separation time was developed to analyze triterpenic acids (TTAs) H. L. by HPLC fluorescence detection simultaneously confirmed post-column atmospheric pressure ionization-mass...

Abstract The herb couple has special clinical significance in reducing the toxicity and increasing efficacy of drugs. combination Radix Angelicae Dahuricae (Baizhi, BZ) Rhizoma Chuanxiong (ChuanXiong, CX) is a traditional couple. performs better than CX extract alone treatment migraine been used for thousands years. However, specific compatibility mechanisms are still unclear. Ligustilide, dl ‐3 ‐n‐ butylphthalide senkyunolide A major active ingredients BZ–CX decoction. comprehensive study...

Abstract Type 2 diabetes (T2DM) is characterized by hyperglycemia resulting from insulin resistance. Jiao-Tai-Wan (JTW), a traditional Chinese medicine consisting of 10:1 formulation Rhizoma Coptidis (RC) and Cortex Cinnamomi (cinnamon) was shown to have hypoglycemic efficacy in type diabetic mouse model. Here we investigated whether glucose consumption insulin-resistant adipocytes could be modulated serum JTW-treated rats, if so, through what mechanism. JTW-medicated prepared rats following...

Endoplasmic reticulum (ER) stress and apoptosis play significant roles in the development of neurotoxicity caused by bupivacaine (BUP). By activating sirtuin 1 (SIRT1), resveratrol (RSV) can regulate various cellular processes associated with anti‑oxidative stress, anti‑apoptosis anti‑inflammatory responses, thereby exerting neuroprotective effects. However, it remains unknown whether activation SIRT1 RSV is able to attenuate BUP‑induced ER apoptosis. Therefore, present study aimed explore...

Pinelliae Rhizoma Praeparatum (PRP) as traditional Chinese medicine had been used for hepatic diseases in combinative forms. However, the effect of PRP was not clear when alone. So to explore hepatoprotective/hepatotoxin is necessary. The activities were investigated acetaminophen-induced injury mice. Liver function markers, oxidative stress markers evaluated. Bile acids metabolic transports and nuclear factor erythroid 2-related 2 (Nrf2) detected. As a drug treatment liver diseases,...

Determining the ordered weighted averaging (OWA) operator weights is important in decision making applications. Several approaches have been proposed literature to obtain associated weights. This paper provides two new disparity models weights, which determine d by considering absolute deviation and relative of any distinct pairs The mathematical improve existing minimize approach chi-square method, suggested Amin Emrouzenjad (2006, 2010) Wang (2007). A numerical example an application...