A5009729539- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Actinomycetales infections and treatment
- Orthopedic Surgery and Rehabilitation
- RNA and protein synthesis mechanisms
- Cancer Immunotherapy and Biomarkers
- Lung Cancer Diagnosis and Treatment
- Lung Cancer Research Studies
- Bacterial biofilms and quorum sensing
- Lung Cancer Treatments and Mutations
- Enzyme Structure and Function
- Escherichia coli research studies
- Pleural and Pulmonary Diseases
- Pneumonia and Respiratory Infections
- Antifungal resistance and susceptibility
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Quinazolinone synthesis and applications
- RNA modifications and cancer
- Metastasis and carcinoma case studies
- Textile materials and evaluations
Tongji University
2016-2025
Shanghai Pulmonary Hospital
2016-2025
Case Western Reserve University
2021-2024
University School
2021-2024
University Medical Center
2023
Hebei Medical University
2020-2023
China Jiliang University
2023
Shanghai Clinical Research Center
2023
Third Hospital of Hebei Medical University
2020-2022
Sun Yat-sen University
2014-2020
Treatment of Mycobacterium abscessus pulmonary infection requires long-term administration multiple antibiotics. Little is known, however, about the impact each antibiotic on treatment outcomes. A retrospective analysis was conducted to evaluate efficacy and adverse effects antibiotics administered in 244 cases M. disease. Only 110 (45.1%) patients met criteria for success. The treating disease continues be unsatisfactory especially infections involving subsp. abscessus. with drug...
Antibiotic-resistant strains of the Gram-negative pathogen Acinetobacter baumannii have emerged as a significant global health threat. One successful therapeutic option to treat bacterial infections has been target ribosome. However, in many cases, multidrug efflux pumps within bacterium recognize and extrude these clinically important antibiotics designed inhibit protein synthesis function Thus, A. other highly drug-resistant is major cause failure drug-based treatments infectious diseases....
The continued challenges of the COVID-19 pandemic combined with growing problem antimicrobial-resistant bacterial infections has severely impacted global health. Specifically, Gram-negative pathogen Klebsiella pneumoniae is one most prevalent causes secondary infection in patients, approximately an 83% mortality rate observed among patients these coinfections. K. belongs to ESKAPE group pathogens, a that commonly gives rise severe are often life-threatening. Recently, carbapenemase...
Biofilm formation is an important factor for Mycobacterium abscessus to resist harsh environment and produce drug resistance. The anti-biofilm activity of a newly designed antibacterial peptide, RP557, was investigated. effect RP557 alone or in combination with several clinically effective antibiotics, including clarithromycin, amikacin, cefoxitin imipenem, on M. growth biofilms determined. Microstructural changes after treatment were observed by scanning electron microscope. the viability...
Lung cancer is the most common cause of cancer-related death globally. There are several reasons for this high mortality rate, including metastasis to multiple organs, especially brain. Exosomes play a pivotal role in tumor by remodeling microenvironment remote target organs and promoting pre-metastatic niche's formation. Since astrocytes indispensable maintaining homeostasis brain microenvironment, it's great interest explore influence lung cell-derived exosomes on further understand...
ABSTRACT Mycobacterium abscessus is a multi‐drug resistant pathogen presenting significant treatment challenges. This study evaluated MRX‐5, an oral prodrug of the leucyl‐tRNA synthetase inhibitor MRX‐6038, for its efficacy against M. both in vitro and vivo. Stability testing MRX‐5 was conducted using liquid chromatography–tandem mass spectrometry Middlebrook 7H9 broth at 35°C. Following this, minimum inhibitory concentrations were determined two reference strains 17 clinical isolates . In...
Chemotherapeutic options against Mycobacterium abscessus infections are very limited. Bedaquiline, a new antituberculosis (anti-TB) drug, is effective for the treatment of multidrug-resistant TB. However, few data available on bedaquiline M. infections. In this study, we determined profile in vitro susceptibility clinical isolates to and investigated potential molecular mechanisms decreased susceptibility. A total 197 were collected from sputum bronchoalveolar fluid patients with lung...
This study aimed to determine the antibiotic susceptibility and resistance related genotypes of Mycobacterium abscessus. One hundred sixty-two clinical isolates were collected. Genomic data obtained by whole genome sequencing. Single nucleotide polymorphism (SNP) analysis was conducted using NCBI GenBank database BLAST algorithm. The following genes interest: erm(41), rrl rrs. Erm(41) further divided into 3 sequevars: erm(41)C28, erm(41)T28, M type [erm(41) with deletions in nucleotides 64...
Antibiotic resistance among bacterial pathogens continues to pose a serious global health threat. Multidrug-resistant (MDR) strains of the Gram-negative organism Acinetobacter baumannii utilize number determinants evade current antibiotics. One major mechanisms employed by these is use multidrug efflux pumps. These pumps extrude xenobiotics directly out cells, resulting in treatment failures when common antibiotics are administered. Here, structure novel tetracycline antibiotic TP-6076,...
Here, we report cryo-EM structures of the AcrD aminoglycoside efflux pump in absence and presence bound gentamicin, posing possibility that this is capable capturing aminoglycosides from central cavity trimer. The results indicate utilizes charged residues to bind export drugs, mediating resistance these antibiotics.
We applied raw human liver microsome lysate to a holey carbon grid and used cryo-electron microscopy (cryo-EM) define its composition. From this sample we identified simultaneously determined high-resolution structural information for ten unique enzymes involved in diverse cellular processes. Notably, the structure of endoplasmic bifunctional protein H6PD, where N- C-terminal domains independently possess glucose-6-phosphate dehydrogenase 6-phosphogluconolactonase enzymatic activity,...
The emergence of clarithromycin resistance is a challenge in treating Mycobacterium abscessus infections. Known mechanisms that contribute to intrinsic focus on rrl gene-related mutations, but resistant clinical isolates often exhibit an inconsistent genotype.In this study, 194 were collected from patients with lung infections and the whole genome each isolate was sequenced. A comprehensive examination molecular underlying performed, combining MIC determination, comparative sequence analysis...
Campylobacter jejuni has emerged as one of the most problematic and highly antibiotic-resistant pathogens, worldwide. The Centers for Disease Control Prevention have designated C. a serious antibiotic resistance threat in United States.
The Mycobacterium tuberculosis (Mtb) pathogen, the causative agent of airborne infection (TB), harbors a number mycobacterial membrane protein large (MmpL) transporters. These proteins can be separated into 2 distinct subclasses, where they perform important functional roles, and thus, are considered potential drug targets to combat TB. Previously, we reported both X-ray cryo-EM structures MmpL3 transporter, providing high-resolution structural information for this subclass MmpL proteins....
A total of 194 Mycobacterium abscessus isolates were collected from patients, and the whole genomes sequenced. Eighty-five (43.8%) showed linezolid (LZD) resistance. Only 8.2% resistant harbored 23S rRNA mutations. Quantitative real-time PCR (qRT-PCR) revealed higher transcriptional levels efflux pumps lmrS mmpL9 in LZD-resistant isolates. Genome comparative analysis identified several new LZD resistance-associated genes. This study highlights role M. proposes potential target genes for...
Mycobacterium abscessus accounts for a large proportion of lung disease cases caused by rapidly growing mycobacteria. The association between clarithromycin sensitivity and treatment outcome is clear. However, M. culture antibiotic susceptibility testing are time-consuming. Clarithromycin genotyping offers an alternate, rapid approach to predicting the efficacy clarithromycin-based therapy. patients were divided into two groups based upon genotype organism isolated. A retrospective analysis...
The aim of the study was to investigate resistant mechanisms and homology imipenem-resistant Acinetobacter baumannii (A. baumannii). A total 46 non-duplicate imipenem‑resistant A. clinical isolates were collected from three tertiary hospitals between July, 2011 June, 2012. minimal inhibitory concentrations (MICs) antimicrobial agents determined using agar dilution method. Phenylalanine‑arginine β-naphthylamide used detect presence efflux pump-mediated mechanism. Polymerase chain reaction...
Refractory rifampicin-resistant/multidrug resistant/extensively-drug resistant tuberculosis (RR/MDR/XDR-TB) were defined as patients infected with Mycobacterium (MTB) to rifampicin(RR-TB), or at least rifampicin and isoniazid (MDR-TB) added fluoroquinolones (FQs) one of second line injectable agents (XDR-TB), a patient for whom an effective regimen (fewer than 4 due adverse events (AEs) multiple drug resistances) cannot be developed. To compare the effectiveness safety bedaquiline...
This study was undertaken to evaluate the utility of matrix-assisted laser desorption ionization-time flight mass spectrometry with Vitek MS Plus system for identifying Mycobacterium abscessus subspecies in order facilitate more rapid and appropriate therapy. A total 175 clinical M. strains were identified by whole-genome sequencing analysis: 139 subsp. 36 massiliense The research-use-only (RUO) Saramis Knowledge Base database v.4.12 modified accordingly adding 40 19 reference spectra...