A5100376970- Diet and metabolism studies
- Medical Imaging Techniques and Applications
- Adipose Tissue and Metabolism
- Advanced MRI Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- MRI in cancer diagnosis
- Metabolism and Genetic Disorders
- Lanthanide and Transition Metal Complexes
- Hepatocellular Carcinoma Treatment and Prognosis
- Radiopharmaceutical Chemistry and Applications
- Ion Transport and Channel Regulation
- Genetics, Aging, and Longevity in Model Organisms
- Genetic and Kidney Cyst Diseases
- NMR spectroscopy and applications
- Advanced Neuroimaging Techniques and Applications
- Animal Virus Infections Studies
- Heat shock proteins research
- Pediatric Urology and Nephrology Studies
- Immune Response and Inflammation
- Ginseng Biological Effects and Applications
- RNA Interference and Gene Delivery
- Hedgehog Signaling Pathway Studies
- Ultrasound and Hyperthermia Applications
- Neurogenesis and neuroplasticity mechanisms
- Metabolomics and Mass Spectrometry Studies
Kobe University
2025
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2024
Beijing Institute of Education
2024
China Three Gorges University
2023
Case Western Reserve University
2012-2022
University School
2017-2019
Xi'an Jiaotong University
2019
Xuan Wu Hospital of the Capital Medical University
2017
Neurological Surgery
2017
The increasing prevalence of ultra-high-field magnetic resonance imaging (UHFMRI) in biomedical research and clinical settings will improve the resolution diagnostic accuracy MRI scans. However, better contrast agents are needed to achieve a satisfactory signal-to-noise ratio. Here, we report synthesis bimodal agent prepared by loading internal cavity tobacco mosaic virus (TMV) nanoparticles with dysprosium (Dy3+) complex near-infrared fluorescence (NIRF) dye Cy7.5. external surface TMV was...
Because of the limitation in treatment window r-tPA (recombinant tissue-type plasminogen activator), development delayed for stroke is needed. In this study, we examined efficacy poststroke (post 3-8 days) sonic hedgehog pathway agonist on functional recovery and underlying mechanisms.We evaluated at 1 month after using locomotion analysis Barnes maze test cognitive function. We used a genetically inducible neural stem cell-specific reporter mouse line (nestin-CreERT2-R26R-YFP) to label...
One of the greatest challenges in nanomedicine is low efficiency with which nanoparticles are delivered to lesions such as tumors vivo. Here, we show that Physalis mottle virus (PhMV)-like can be developed bimodal contrast agents achieve long circulation, specific targeting capability, and efficient delivery The self-assembling coat protein nanostructure offers various opportunities modify internal external surfaces separately. After loading cavity particles fluorescent dye Cy5.5...
The mechanistic link of ketosis to neuroprotection under certain pathological conditions continues be explored. We investigated whether chronic induced by ketogenic diet results in the partitioning ketone bodies toward oxidative metabolism brain. hypothesized that diet-induced increased shunting citric acid cycle and amino acids with decreased carbon from glucose. Rats were fed standard (STD) or (KG) diets for 3.5 weeks then infused [U-(13) C]glucose C]acetoacetate tracers. Concentrations...
Aquaporin-4 (AQP4) plays an important role in regulating water exchange across the blood-brain barrier (BBB) and brain-cerebrospinal fluid interface. Studies on AQP-4 knockout mice (AQP4-KO) have reported considerable protection from brain edema induced by acute intoxication ischemic stroke, identifying AQP4 as a potential target for therapeutic interventions. However, long-term effects of chronic suppression are yet to be elucidated. In current study, we evaluated physiological structural...
Abstract Ambient air temperature is a key factor affecting human health. Female reproductive disorders are representative health risk events under low temperature. However, the mechanism involving in cold-induced female remains largely unknown. mice were intermittently exposed to cold conditions (4 °C) address of on system. Primary granulosa cells (GCs) prepared and cultured (35 or β3-adrenoreceptor agonist, isoproterenol, mimic condition exposure. Western-blot, RT-PCR, co-IP, ELISA,...
Utilizing mouse models provides excellent immunological and experimental tools to study oral immune responses. However for functional assays, isolating T lymphocytes from the tissues has proved be challenging due absence of reliable methods that yield viable cells with consistency. To adaptive cell interactions in mucosal tissues, it is necessary isolate a good viability them at single level.We have established an improved method cells, including Tregs Th17 intra-epithelial niches lamina...
Synchronous assessment of multiple MRI contrast agents in a single scanning session would provide new "multi-color" imaging capability similar to fluorescence but with high spatiotemporal resolution and unlimited depth. This multi-agent technology enable whole class basic science clinical experiments that simultaneously explore physiologic/molecular events vivo. Unfortunately, conventional acquisition techniques are only capable detecting quantifying one paramagnetic agent at time. Herein,...
Increasing prevalence of obesity‑induced non‑alcoholic fatty liver disease (NAFLD) and steatohepatitis (NASH) has been reported. Ascorbic acid (AA), also known as vitamin C, an excellent antioxidant, shown to exert beneficial effects on NAFLD; however, the underlying mechanisms are yet be fully elucidated. In present study, role AA cell stress in tumor necrosis factor α (TNFα)‑treated HepG2 cells was investigated. Our findings revealed that exposure effectively ameliorated TNFα‑induced...
Clinical diagnosis of hepatocellular carcinoma (HCC) relies heavily on radiological imaging. However, information pertaining to liver cancer treatment such as the proliferation status is lacking. Imaging tumor can be valuable in patient management. This study investigated 18F-labeled clofarabine ([18F]CFA) targeting deoxycytidine kinase (dCK) for PET imaging dCK-dependent HCC. Since clinical scans showed a high background uptake [18F]CFA, aim this was reduce uptake. A clinically relevant...
Purpose The current study aimed to develop a three‐dimensional (3D) dynamic oxygen‐17 ( 17 O) MR imaging method with high temporal and spatial resolution delineate the kinetics of O water uptake washout in brains mice glioblastoma (GBM). Methods A 3D stack‐of‐stars golden‐ratio–based radial sampling scheme was employed acquire signal vivo. k‐space–weighted image reconstruction used improve while preserving resolution. Simulation studies were performed validate method. Using this method, GBM...
[11C]methionine ([11C]Met) was used for cancer imaging based on upregulated amino acid transport and protein synthesis in different tumor types. However, the short half-life of 11C decay limited further clinical development [11C]Met. Synthetic analog anti-1-amino-3-[18F]fluoro-cyclobutyl-1-carboxylic ([18F]FCABC) developed FDA-approved PET recurrent prostate cancer. This study investigated "repurposed" [18F]FACBC primary liver such as hepatocellular carcinoma (HCC) comparison with [11C]Met...
Aging and obesity are closely related, the prevalence of metabolic diseases rapidly increasing with an aging population worldwide. A key change in adipose tissue during process is decline lipolysis, excitation sympathetic nerve system a pathway to promote lipolysis. Previous studies have shown that total saponins from Panax japonicus (TSPJs) can prevent mice high-fat diet by fat decomposition. In this work, we examine regulatory effects TSPJs on lipolysis state explore possible mechanisms....
X-linked adrenoleukodystrophy is an inherited disease caused by abnormal accumulation of very long chain fatty acids. The diagnosis can be confirmed with the mutation ABCD1 gene. main symptom spastic paraparesis, and autonomic dysfunction rare in adrenoleukodystrophy. Here, we presented X-ALD case a 46-year-old Asian male severe dysfunction. Impairment nervous system may closely relate to mitochondrial defect.
We have previously reported decreased cerebral metabolic rate of glucose in brain diet‐induced ketotic rats which inversely correlated with blood ketone levels. These data suggest a “switch” substrate utilization from to bodies. The effect ketosis on intermediary metabolism remains be explored. In this study we investigated the effects chronic partitioning oxidation. characterized 13 C fractional contribution ketones and into citric acid cycle (CAC), GABA neuro‐amino acids rat using...
In the brain, Aquaporin 4 (AQP4) is highly expressed in blood–brain barrier and brain–CSF interfaces. AQP4 functions as an efficient water‐selective transporting protein plays important role cerebral water balance. It has been shown that knocking out of mice reduces brain edema after acute intoxication ischemic stroke. The specific localization to these anatomical cellular regions central nervous system may also suggest a for gas channel facilitate oxygen transport. this study we...
We had previously reported a decrease of glucose oxidation which was replaced by an increase ketone body in ketotic rat brain. The metabolic effect ketosis on the TCA cycle flux remains to be explored. GABA, neurotransmitter brain, is balanced supply carbons from cycle. investigated carbon ketones and into GABA with ketosis. Method Tracer infusions [U‐ 13 C]acetoacetate or C]glucose were used measure C‐label incorporation related intermediates cortex diet‐induced rats. GC‐MS stable...