A5012158103- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Synthetic Organic Chemistry Methods
- Fungal Biology and Applications
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Cancer therapeutics and mechanisms
- Carbohydrate Chemistry and Synthesis
- Plant biochemistry and biosynthesis
- Marine Sponges and Natural Products
- Microbial Metabolism and Applications
- Bioactive Compounds and Antitumor Agents
- Natural product bioactivities and synthesis
- Plant Disease Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Studies on Chitinases and Chitosanases
- Nematode management and characterization studies
- Bacteriophages and microbial interactions
- Helminth infection and control
- RNA modifications and cancer
- Fungal Plant Pathogen Control
- Biochemical and Molecular Research
- Traditional and Medicinal Uses of Annonaceae
- Sulfur-Based Synthesis Techniques
- Mycobacterium research and diagnosis
Huzhou University
2020-2025
Taizhou University
2023
Zhejiang Hisun Pharmaceutical (China)
2013-2020
China Jiliang University
2019
Wuhan University
2009-2011
A pink-pigmented, Gram-negative, rod-shaped, motile, strictly aerobic bacterium, designated strain 311(T), was isolated from desert sand in Xinjiang, China. Phylogenetic analyses based on 16S rRNA gene sequences showed that 311(T) related closely to Cesiribacter andamanensis AMV16(T) (94.6% similarity). The DNA G+C content of 47.1 mol% and the major respiratory quinone menaquinone 7 (MK-7). main cellular fatty acids were C(16:1)ω5c (29.9%), iso-C(15:0) (21.9%), iso-C(17:0) 3-OH (13.3%)...
Gummy stem blight (GSB), which is caused by Stagonosporopsis cucurbitacearum, threatens pumpkin yields and agriculture. Effective, safe antifungal agents are urgently needed. In this study, fermentation broth supernatant of Streptomyces sp. NEAU-T55 demonstrated considerable activity against S. cucurbitacearum. Activity-guided isolation identified 2 new 14 known compounds, with (+)-methyl nonactate (10) determined as the main active ingredient. This compound exhibited strong (EC50 = 0.12 μg...
is an important fungal pathogen that causes gray mold disease in plants. Previously,
A novel strain, designated HY-50R T , isolated from soil of a Euphrates poplar ( Populus euphratica ) forest in Xinjiang, China, was characterized using polyphasic taxonomic approach. Cells the isolate were Gram-reaction-negative, strictly aerobic, rod-shaped, non-motile, oxidase-negative and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that member phylum Bacteroidetes its closest relatives being Niastella populi THYL-44 (93.6 % similarity),...
An efficient and novel visible-light-induced oxo-amination of terminal alkenes for the construction α-amino ketones with easily accessible green O2 as oxygen source has been developed. The transformation possesses advantages operational simplicity, a broad substrate scope, high atom economy, mild reaction conditions. mechanistic studies reveal that an energy transfer process probably occurs in initial stage, proceeds via β-scission alkoxyl radical species.
A novel strain, HY-22R(T), was isolated from soil of a Euphrates poplar forest in Xinjiang, China. The cells were Gram-positive-staining, rod-shaped and motile by means peritrichous flagella. Growth occurred at 10-37 degrees C (optimum 30 C), pH 7.0-8.0 7.0) with 0-1 % NaCl. phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HY-22R(T) closely related to Cohnella phaseoli GSPC1(T) (96.3 sequence similarity). major respiratory quinone MK-7 the predominant fatty acids...
The mangrove ecosystem is a rich resource for the discovery of actinomycetes with potential applications in pharmaceutical science. Besides genus Streptomyces, Micromonospora also source new bioactive agents. We screened from rhizosphere soil plants Fujian province, China, and 51 strains were obtained. Among them, extracts 12 isolates inhibited growth human lung carcinoma A549 cells. Strain 110B exhibited better cytotoxic activity, its constituents investigated. Consequently, three...
Oomycetes are well-known phytopathogens that seriously threaten many important crops worldwide. In this study, the endophytic actinobacterium Streptomyces sp. NEAU-S7GS2 demonstrated significant antagonistic activity against Phytophthora and Pythium showed a potent biocontrol effect on suppression of soybean phytophthora root rot pepper blight. Two compounds were subsequently isolated as main active components by bioassay-guided fractionation identified lydicamycins A B. These two high...
A Gram-negative, aerobic, motile, Sphingomonas-like rod, strain 10-1-84(T), was isolated from a sand sample collected the desert of Xinjiang, China. The isolate contained Q-10 as predominant ubiquinone and C(18 : 1)ω7c, C(16 0), 1)ω7c C(14 0) 2-OH major fatty acids. polyamine pattern predominantly sym-homospermidine. main polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine an...
Pseudouridine-incorporated mRNA vaccines can enhance protein expression and reduce immunogenicity, leading to a high demand for pseudouridine be used in drug production. To achieve the low-cost production of pseudouridine, Escherichia coli was systematically modified utilize inexpensive raw materials efficiently produce pseudouridine. First, pyrimidine biosynthesis pathway, genes related precursor competing pathway negative regulator were deleted, which increased Second, two critical genes,...
Abstract A new bacterial strain, designated HM134 T , was isolated from a sample of soil collected Chinese mangrove Avicennia marina forest. Assessed by polyphasic approach, the taxonomy strain found to be associated with range phylogenetic and chemotaxonomic properties consistent genus Micromonospora . Phylogenetic analysis based on 16s rRNA gene sequence indicated that formed distinct lineage most closely related species, including M rifamycinica AM105 wenchangensis CCTCC AA 2012002 M....
Strain 15-4(T), a Gram-stain-negative, rod-shaped, non-motile bacterial strain that produced flexirubin-type pigments, was isolated from Tibet Province, China, and characterized by using polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated the isolate belonged to family Chitinophagaceae, phylum 'Bacteroidetes', related members of genus Niabella, with sequence similarities ranging 94.1 96.4 %. 15-4(T) contained MK-7 as predominant menaquinone its DNA...
Novel antilipid peroxidative carbazole alkaloids, antiostatin A5 (1), A6 (2), and (±)-morindolestatin (3), were isolated from a new soil-derived Streptomyces sp. Compound 2 possesses an unusual cyclohexene side chain. 3 was pair of enantiomers featuring unprecedented [1,4]oxazino[2,3-c]carbazole ring system. The absolute configuration determined by online HPLC-ECD ECD calculation. A racemization mechanism putative biosynthetic pathway are discussed.
Bioinformatics analysis of a whole genome sequence coupled with HPLC-DAD revealed that Streptomyces sp. Hu103 has the capacity to produce skyllamycin analogues. A subsequent chemical investigation this strain yielded four new cinnamoyl-containing cyclopeptides, anulamycins A–D (1–4), two linear peptides, E and F (5 6), known skyllamycins (7) B (8). Their structures including absolute configurations were elucidated by detailed NMR HRESIMS/MS spectroscopic data advanced Marfey’s method....
Tenvermectins A and B (TVMs B) are hybrid natural compounds of avermectins milbemycins with enhanced insecticidal activity. Aiming at obtaining a strain for the production higher activity metabolite-TVM-B as major constituent, recombinant Streptomyces avermitilis HU02 was constructed by domain swapping strategy in which milA1-AT0 gene S. MHJ1011 replaced eryA1-AT0 from Saccharopolyspora erythraea ATCC 40137. Chemical investigation on culture led to isolation large amount TVM-B trace amounts...
Streptomyces is an effective source of new natural bioherbicides. In this study, a novel isolated strain NEAU-HV44 showed strong inhibitory activity against Amaranthus retroflexus L. and was concluded to the genus Streptomyces. Strain fermentation conditions were optimized maximize herbicidal activity. The supernatant could significantly control growth weeds (A. L., Setaria viridis, Portulaca oleracea Chenopodium album) crops (maize, soybean, wheat, Chinese cabbage, cucumber, tomato, romaine...