Arachidonate 5-lipoxygenase was isolated from guinea pig peritoneal polymorphonuclear leukocytes, and partially purified a high speed supernatant of homogenate.5-Hydroperoxy-6,8,11,14-eicosatetraenoic acid predominant reaction product.The enzyme required specifically calcium ion (20 pM for half-maximal activity), other divalent cations such as magnesium cobalt were ineffective.The calcium-dependent stimulated by several nucleotides, most prominently ATP in the order 1...

Nucleotides from yeast RNA were evaluated for their ability to enhance the non‐specific immune responses in carp, Cyprinus carpio . Oral administration of nucleotides fish daily 3 days resulted enhanced phagocytic and nitroblue tetrazolium (NBT) activities kidney cells. This activation cells was observed at least 10 post‐treatment. The serum complement lysozyme also increased treated with nucleotides. Furthermore, number Aeromonas hydrophila nucleotide‐treated significantly decreased blood,...

Abstract Purpose: The anaphylatoxin C5a is a chemoattractant that induces leukocyte migration via receptor (C5aR). There emerging evidence generated in the cancer microenvironment. We therefore sought C5aR expression and direct influence of C5a–C5aR axis on cells. Experimental Design: was investigated human tissues cell lines. Effects stimulation cells were studied by cytoskeletal rearrangement, time-lapse analysis, Matrigel chamber assay, invasion nude mouse comparison C5aR-expressing with...

Abstract Aim: We used a model of chronic unloading followed by reloading to examine the apoptotic responses associated with soleus muscle atrophy and subsequent recovery. Methods: Male Wistar rats were subjected hindlimb (HU) for 2 weeks 0, 3, 7 14 days. One‐half HU‐reloaded administered cyclosporine A (CsA), calcineurin (CaN) inhibitor. Results: There was fibre (73%) decrease in slow type I fibre/myosin heavy chain (MyHC) composition after HU. Fibre size MyHC recovered near age‐matched...

The mechanism of anti-tumour activity by BCG is not known clearly. However, many studies suggest that immunological response related to effectiveness intravesical instillation in the therapy for superficial bladder carcinoma. Peripheral blood mononuclear cells (PBMC), urine and serum were obtained from patients with carcinoma at various times during course instillation. Urine showed increased levels IL-1beta, IL-2, IL-6, tumour necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma)...

Changes in the expression of heat shock protein 72 (HSP72) response to atrophic‐inducing perturbations muscle involving chronic mechanical unloading and denervation were determined. Adult male Wistar rats assigned randomly a sedentary cage control (CON), hind limb (HU, via tail suspension), HU plus tenotomy (HU + TEN), DEN), or TEN DEN group. Tenotomy involved cutting Achilles tendon removing segment sciatic nerve, respectively. After 5 days, HSP72 levels soleus groups 42 ( P < 0.05) 53%...

Clenbuterol, a beta2-agonist, administration results in hypertrophy of fast fibres and an increase the myosin heavy chain (MHC) composition both slow muscles. The present study was designed to determine phenotypic response at single fibre level. Clenbuterol added drinking water (30 mg L(-1)) adult male Wistar rats for 4 weeks. Single from soleus muscle control (10 rats; 555 fibres) clenbuterol-treated 577 were dissected their MHC isoform determined using sodium dodecyl...

Transurethral resection (TUR) is the standard operation used for non-muscle invasive bladder cancer (NMIBC). Although most solid tumors are principally removed via single block without incising mass, disruption of lesion unavoidable in traditional TUR. Furthermore, pathological diagnosis often difficult due to heat-related denaturation tissues transurethral en-bloc useful judging tumor invasion, it associated with a prolonged operative duration. We attempted show safety and usefulness...

Natural cell-mediated cytotoxicity against NK-resistant target tumor cells was found in the peripheral blood of tumor-bearing patients approximately 1 mo after combined chemotherapy. The recognition specificity these effector broad and had no restriction. From experiments negative selection with mAbs complements, newly developed killer chemotherapy were thought to be LAK-like cells. Contribution mechanism action anticancer drugs remains clarified.

C-terminus of S19 ribosomal protein (RP S19) endows the cross-linked homodimer with a dual effect on C5a receptor in leucocyte chemoattraction; agonistic monocyte receptor, and antagonistic neutrophil receptor. exhibits uniform this both cell types. We have currently prepared recombinant C5a-chimeric bearing RP (C5a/RP chimera) to be used as substitute dimer. In vitro, chimera similarly inhibited intracellular Ca(2+) mobilization neutrophils induced by dimer did. guinea pig skin, 10(-7) M...

Autoimmune diseases are caused by immune complex‑induced activation of the complement system and subsequent inflammation. Recent studies have revealed an association between autoimmune worse survival in patients with cancer; however, underlying mechanism is still unknown. The C5a‑C5a receptor (C5aR) has been shown to enhance cancer activity recruit myeloid‑derived suppressor cells (MDSCs) that suppress anti‑tumor response. Arthus reaction inflammation complex thus a model diseases. To...

Abstract The signal transducer and activator of transcription 3 (STAT3) signaling pathway is a key mediator cancer cell proliferation, survival invasion. Aberrant STAT3 has been demonstrated in various malignant cancers. YHO‐1701 novel quinolinecarboxamide derivative generated from STX‐0119. Here, we examined the effect on evaluated antitumor activity as single agent combination. inhibited STAT3‐SH2 binding to phospho‐Tyr peptide selectively more potently than STX‐0119 biochemical assays....

We examined the involvement of acquired immune response in therapeutic effect systemic tumor chemotherapy with cis-diamminedichloroplatinum (II) (cis-DDP) using a mouse syngeneic cancer transplantation model. The observed BALB/c mice was negligible athymic mice. became obvious when were immunologically reconstituted splenocytes containing CD4+ T cells which successful exhibited cell line-specific immunity to second transplantation. Splenocytes these cytotoxicity. After cis-DDP treatment, an...

MYH9-related disease is a rare genetic disorder characterized by macrothrombocytopenia, with frequent proteinuric nephropathy, hearing loss, and cataract. Although nephropathy usually progresses to renal failure, there no established treatment for the nephropathy. We herein describe case of 19-year-old man carrying an E1841K MYH9 mutation, who developed persistent proteinuria. The patient was diagnosed early-stage based on histological examination kidney biopsy specimen. treated enalapril,...

To compare the urinary pH, recurrence-free survival (RFS), and safety of adjuvant intravesical therapy in patients with non-muscle-invasive bladder cancer (NMIBC) receiving mitomycin C (MMC) MMC + cytosine arabinoside (Ara-C) therapy.A total 165 NMIBC from six hospitals were randomly allocated to two groups: weekly instillation Ara-C (30 mg/30 mL 200 mg/10 mL) for 6 weeks same schedule mg/40 mL). The primary outcome was RFS, secondary outcomes pH toxicity groups.A 81 87 randomised into...

Abstract Signal transducer and activator of transcription 3 (STAT3) plays a critical role in regulating cell growth, survival, metastasis. STAT3 signaling is constitutively activated various types hematologic or solid malignancies. YHO-1701 has been developed as an orally available inhibitor. Herein, combination with molecular-targeted agents was evaluated. Additive synergistic effects were observed broad spectrum “combination treatment + line” pairs. Of particular interest the effect when...

Summary Reasons for performing study: In man, muscle protein synthesis is accelerated by administering amino acids (AA) and glucose (Glu), because increased availability of insulin secretion, known to have a anabolic effect. However, in the horse, effect on hypertrophy such nutrition management following exercise unknown. Objectives: To determine AA Glu administration turnover horses. We hypothesise that after effects horses, as already shown man other animals. Methods: Measurements rate...

Isolated peripheral neutrophils spontaneously underwent apoptosis in association with extra‐cellular liberation of the monocyte‐attracting ribosomal protein S19 ( RP ) oligomers. This was prevented by simultaneous presence anti‐ antibodies or a C5a receptor antagonist, but promoted supplementing extrinsic Transformed HL ‐60 cells to over‐produce G ln137 A sn‐mutant were differentiated neutrophil‐like cells. The gained resistance against spontaneous concomitant generation non‐functional When...

Methods for determining the origin of BK virus (BKV)-infected cells (decoy cells) in clinical urine samples have not been established although they could enhance diagnosis BKV infection immunocompromised patients.We performed simultaneous immunostaining with anti-S100P (a urothelial marker) and anti-SV40 antibodies 66 exhibiting SV40 positivity a decoy-cell appearance on Papanicolaou staining. The voided included seven cases renal transplantation, 47 cancer therapy 12 non-neoplastic disease....

We examined the roles of a chemotherapeutic reagent in both inducing apoptosis and conferring acquired tumor immunity using mouse syngeneic transplantation models. A reagent, cis-diamminedichloroplatinum (II), effectively induced Colon-26 cells originating from BALB/c mouse. Three intradermal inoculations (II)-treated conferred against viable mice but not athymic mice. The was cell specific adoptively transferable with splenic cells. next involvement cross-linked ribosomal protein S19 dimer...