A5006561776- Ubiquitin and proteasome pathways
- Organophosphorus compounds synthesis
- Autophagy in Disease and Therapy
- Pediatric health and respiratory diseases
- Phosphorus compounds and reactions
- Peptidase Inhibition and Analysis
- Semiconductor Quantum Structures and Devices
- Hippo pathway signaling and YAP/TAZ
- Protein Degradation and Inhibitors
- Photovoltaic Systems and Sustainability
- Endoplasmic Reticulum Stress and Disease
- Synthesis and characterization of novel inorganic/organometallic compounds
- Lipid Membrane Structure and Behavior
- Advanced Power Amplifier Design
- Sharing Economy and Platforms
- Robotics and Automated Systems
- Elevator Systems and Control
- Magneto-Optical Properties and Applications
- Chalcogenide Semiconductor Thin Films
- Supramolecular Self-Assembly in Materials
- Silicon Carbide Semiconductor Technologies
- Helicobacter pylori-related gastroenterology studies
- Protein Structure and Dynamics
- Radio Frequency Integrated Circuit Design
- DNA Repair Mechanisms
The University of Tokyo
2001-2025
Tokyo Metropolitan Institute of Medical Science
2021
The University of Texas at Austin
2019-2020
Ryukoku University
2014-2015
Tokyo Medical and Dental University
2007-2011
Tohoku University
2002
Niigata University
1995
Kagawa National Children's Hospital
1987
Abstract Proteins are targeted to the proteasome by attachment of ubiquitin chains, which markedly varied in structure. Three subunits–Rpn10, Rpn13, and Rpn1–can recognize chains. Here we report that proteins with single chains K48-linked for degradation almost exclusively through binding Rpn10. Rpn1 can act as a co-receptor Rpn10 K63 certain other chain types. Differences targeting do not correlate affinity receptors. Surprisingly, steady-state assays Rpn13 retarded various single-chain...
Yes-associated protein (YAP) is a recently discovered growth-promoting transcription coactivator that has been shown to regulate the malignancy of various cancers. How YAP regulated not fully understood. Here, we show one factors regulating phosphatidylserine (PS) in recycling endosomes (REs). We use proximity biotinylation find proteins proximal PS. Among these are and multiple related signalling. Knockdown ATP8A1 (an RE PS-flippase) or evectin-2 RE-resident protein) masking PS cytoplasmic...
Abstract Retinoblastoma protein (Rb) is a tumor suppressor that binds and represses E2F transcription factors. In cervical cancer cells, human papilloma virus (HPV) E7 to Rb, releasing it from promote cell cycle progression, inducing ubiquitination of Rb. E7-mediated proteasomal degradation Rb requires action by another protease, calpain, which cleaves after Lys 810. However, not clear why cleavage required for degradation. Here, we report the proteasome cannot initiate efficiently on...
The accumulation of defective polypeptides in cells is a major cause various diseases. However, probing proteins difficult because no currently available method can retrieve unstable translational products soluble state. To overcome this issue, there need for molecular device specific to structurally polypeptides. In study, we developed an artificial protein architecture comprising tandemly aligned BAG6 Domain I, minimum substrate recognition platform responsible quality control. This...
Protein ubiquitylation is maintained by a dynamic balance of the conjugation and deconjugation ubiquitin. It remains unclear how deubiquitylation-stabilized substrates are directed for degradation. Branched ubiquitin chains promote substrate degradation through proteasome. TRIP12 UBR5 HECT-type E3 ligases, which specific lysine 29 (K29) 48 (K48) linkages, respectively. Here, we show that deubiquitylase (DUB) OTUD5 cooperatively modified UBR5, resulting in K29/K48 branched accelerated...
RNA-protein interactions (RPIs) are as important protein-protein (PPIs) for the formation of membraneless organelles (MLOs) and play a vital role in various biological processes. Despite remarkable advances PPI analysis technologies recent years, development RPI tools has lagged behind. To advance analysis, we integrated three established tools-bimolecular fluorescence complementation (BiFC), NanoBiT, split-TurboID-with RNA-targeting CRISPR/Cas13. We applied these to analyze paraspeckles,...
Abstract In Ribosome-associated Quality Control (RQC), K63-linked polyubiquitination of uS10 on the stalled ribosome is crucial for recruiting RQC-trigger (RQT) complex. However, mechanisms governing maintenance and recycling polyubiquitin architecture colliding ribosomes remain unclear. Here we demonstrate that two deubiquitinating enzymes (DUBs), Ubp2 Ubp3, play key roles in editing chains uS10, thereby contributing to promotion RQC activity. Specifically, eliminates chain from free 40S...
It has been recently demonstrated that NKG2D is an activating costimulatory receptor on natural killer (NK) cells, T (NKT) activated CD8(+) and gammadelta which respond to cellular stress, such as inflammation, transformation, infection. Here we show intestinal inflammation in colitic SCID mice induced by adoptive transfer of CD4(+)CD45RB(high) cells characterized significant increase CD4(+)NKG2D(+) constitutive expression ligands, H60, Mult-1, Rae-1, lamina propria CD11c(+) dendritic cells....
Lipid giant vesicles (GVs) exhibit biologically relevant morphological dynamics such as growth and division under certain conditions without any sophisticated molecular machineries employed by the current organisms. Nonequilibrium are essential for emergence of dynamic behaviors, which normally generated addition stimulating materials or change some physical conditions. Therefore, an experimental method that allows flexible control external is desirable. Here we report a new simple perfusion...
Abstract Protein quality control is an important mechanism to maintain cellular homeostasis. Damaged proteins have be restored or eliminated by degradation, which mainly achieved molecular chaperones and the ubiquitin-proteasome system. The NAD + -dependent deacetylase Sirt1 has been reported play positive roles in regulation of homeostasis response various stresses. However, its contribution protein remains unexplored. Here we show that involved both Hsp70-dependent Hsp70-independent...
The proteasome is the proteolytic machinery at center of regulated intracellular protein degradation and participates in various cellular processes. Maintaining quality therefore important for proper cell function. It unclear, however, how proteasomes change over time aged are disposed. Here, we show that undergoes specific biochemical alterations as it ages. We generated Rpn11-Flag/enhanced green fluorescent (EGFP) tag-exchangeable knock-in mice established a method selective purification...
The HECT-type ubiquitin E3 ligases including ITCH regulate many aspects of cellular function through ubiquitinating various substrates. These are known to be allosterically autoinhibited and require an activator protein fully achieve the ubiquitination their Here we demonstrate that FAM189A2, a downregulated gene in breast cancer, encodes new type activator. FAM189A2 is transmembrane harboring PPxY motifs, motifs mediate its association with by ITCH. also associates Epsin accumulates early...
Abstract Background Epigenetic dysregulation affecting oncogenic transcription and DNA damage response is a hallmark of cancer. The histone demethylase KDM4B, factor regulating these processes, plays important roles in estrogen receptor-mediated repair breast However, how phospho-signal transduction affects epigenetic regulation not fully understood. Here we found that KDM4B phosphorylation by ribosomal S6 kinase (RSK), downstream effector the Ras/MAPK pathway, critical for function to...
Antimicrobial peptides, including beta-defensins, are thought to be effective agents against opportunistic infections. In humans, three beta-defensins have been identified. The first human beta-defensin, hBD-1, is predominantly expressed in epithelia of the urogenital tract and has reported constitutive. second third hBD-2 hBD-3, were isolated from psoriatic skin found respiratory tract. Of note, gene expression inducible by various proinflammatory such as TNF-alpha, IL-1 beta, IL-8, LPS,...
Nanostructures of diluted magnetic semiconductors are fabricated by molecular beam epitaxy and electron lithography. Excitons in these nanostructures show the low dimensional confinement effects spin interaction with ions. The magneto-optical properties formation relaxation dynamics excitons studied ultrafast time-resolved spectroscopy.