A5031307021- Helicobacter pylori-related gastroenterology studies
- Cancer Cells and Metastasis
- Inflammatory Bowel Disease
- Immune Cell Function and Interaction
- Digestive system and related health
- Cancer Research and Treatments
- T-cell and B-cell Immunology
- Microscopic Colitis
- Genetic factors in colorectal cancer
- IL-33, ST2, and ILC Pathways
- Gastric Cancer Management and Outcomes
- Gastrointestinal disorders and treatments
- Cancer Genomics and Diagnostics
- Gastrointestinal Bleeding Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Gastrointestinal Tumor Research and Treatment
- Hepatitis C virus research
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- Liver Disease Diagnosis and Treatment
- Cytokine Signaling Pathways and Interactions
- Colorectal Cancer Treatments and Studies
- Eosinophilic Esophagitis
- Liver Diseases and Immunity
- Cancer, Hypoxia, and Metabolism
University of Tsukuba
2021-2025
University of Tsukuba Hospital
2024-2025
Suzuki (Japan)
2024
Weatherford College
2024
University Gastroenterology
2024
Tokyo Medical and Dental University
2012-2021
The University of Tokyo
2014-2021
National Institute of Diabetes and Digestive and Kidney Diseases
2016-2020
ProQuest (United States)
2016-2020
Hudson Institute
2020
Abstract This study tested the effects of hydrostatic pressure (10 MPa) on adult articular chondrocyte mRNA and extracellular matrix synthesis in vitro . High density primary cultures bovine chondrocytes were exposed to applied intermittently at 1 Hz or constantly for 4 hours serum‐free medium containing 1% fetal serum, mRNAs aggrecan, types I II collagen, β‐actin analyzed by Northern blots quantified slot blots. Proteoglycan was 35 SO uptake into cetylpyridinium chloride‐precipitable...
Notch signaling regulates cell differentiation and proliferation, contributing to the maintenance of diverse tissues including intestinal epithelia. However, its role in tissue regeneration is less understood. Here, we show that activated a greater number epithelial cells inflamed mucosa colitis. Inhibition activation vivo using gamma-secretase inhibitor resulted severe exacerbation colitis attributable loss regenerative response within layer. Activation supported by suppressing goblet...
Treatment with anti-TNF-alpha MAb has been accepted as a successful maintenance therapy for patients inflammatory bowel diseases (IBD). Moreover, it recently reported that blockade of TNF receptor (TNFR) 1 signaling in infiltrating hematopoietic cells may prevent the development colitis-associated cancer (CAC). However, remains unclear whether TNF-alpha epithelial is involved CAC. To investigate this, we studied effects an animal model CAC by administration azoxymethane (AOM) followed...
The transcription factor Atoh1/Hath1 plays crucial roles in the differentiation program of human intestinal epithelium cells (IECs). Although previous studies have indicated that Notch signal suppresses IEC, mechanism by which it does so remains unknown. This study shows undifferentiated state is maintained suppression Hath1 gene intestine. To assess effect signaling, doxycycline-induced expression intracellular domain (NICD) and Hes1 were generated LS174T. was analyzed quantitative...
Autophagy plays important roles in metabolism, differentiation, and survival T cells. TNFAIP3/A20 is a ubiquitin-editing enzyme that thought to be negative regulator of autophagy cell lines. However, the role TNFAIP3 remains unclear. To determine whether regulates CD4 cells, we first analyzed Tnfaip3-deficient naïve cells vitro. We demonstrated exhibited reduced MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3) puncta formation, increased mitochondrial content, exaggerated...
Ubiquitination is a crucial post-translational modification; however, the functions of ubiquitin-coding genes remain unclear. UBA52 encodes fusion protein comprising ubiquitin at N-terminus and ribosomal L40 (RPL40) C-terminus. Here we showed that Uba52-deficient mice die during embryogenesis. UBA52-deficient cells exhibited normal levels total ubiquitin. However, displayed decreased synthesis cell-cycle arrest. The overexpression ameliorated arrest caused by deficiency. Surprisingly, RPL40...
Summary Background and Aims While filgotinib, an oral Janus kinase (JAK) 1 preferential inhibitor, is approved for moderately to severely active ulcerative colitis (UC), real‐world studies assessing its short‐ long‐term efficacy safety are limited. Methods This a multicenter, retrospective study of UC patients who started filgotinib between March 2022 September 2023. The primary outcome was clinical remission, defined as partial Mayo score ≤1 with rectal bleeding 0, or Simple Clinical...
Maintenance of the intestinal epithelium is based on well-balanced molecular mechanisms that confer stable and continuous supply specialized epithelial cell lineages from multipotent progenitors. Lineage commitment decisions in system involve multiple regulatory systems interplay with each other to establish cellular identities. Here, we demonstrate microRNA could be involved differentiation, microRNA-194 (miR-194) highly induced during this process. To investigate inducible expression...
Intestinal epithelial cell-derived interleukin (IL)-7 functions as a pleiotropic and nonredundant cytokine in the human intestinal mucosa; however, molecular basis of its production has remained totally unknown. We here showed that cells both constitutively when induced by gamma interferon (IFN-gamma) produced IL-7, while several other factors we tested had no effect. Transcriptional regulation via an IFN regulatory factor element (IRF-E) on 5' flanking region, which lacks canonical core...
Abstract Genetic alterations in the TGFβ signaling pathway combination with oncogenic lead to cancer development intestines. However, mechanisms of suppression malignant progression intestinal tumors have not yet been fully understood. We examined ApcΔ716 Tgfbr2ΔIEC compound mutant mice that carry mutations Apc and Tgfbr2 genes epithelial cells. found inflammatory microenvironment only invasive adenocarcinomas but noninvasive benign polyps same mice. thus treated simple dextran sodium...
Intestinal stem cells (ISCs) play indispensable roles in the maintenance of homeostasis, and also regeneration damaged intestinal epithelia. However, whether inflammatory environment Crohn's disease (CD) affects properties resident small remain uncertain.CD patient-derived organoids were established from enteroscopic biopsy specimens taken active lesions (aCD-SIO), or mucosa under remission (rCD-SIO). Expression ISC-marker genes those was examined by immunohistochemistry, microfluid-based...
ATOH1 is a master transcription factor for the secretory lineage differentiation of intestinal epithelial cells (IECs). However, comprehensive contribution ATOH1+ IECs to homeostasis, repair, and tumorigenesis epithelium remains uncertain. Through our cell-lineage tracing, we show here that definite number retain stem cell properties can form ATOH1+IEC-derived clonal ribbons (ATOH1+ICRs) under completely homeostatic conditions. Interestingly, colonic appeared exhibit their function more...
Abstract It is well known that immune responses in the intestine remain a state of controlled inflammation, suggesting not only does active suppression by regulatory T (TREG) cells play an important role normal intestinal homeostasis, but also its dysregulation response leads to development inflammatory bowel disease. In this study, we demonstrate murine CD4+CD25+ residing lamina propria (LP) constitutively express CTLA-4, glucocorticoid-induced TNFR, and Foxp3 suppress proliferation...
Osteoarthritis is a disease in which articular cartilage metabolism altered, leading to destruction. As insulin-like growth factor-I (IGF-I) the major anabolic mediator for cartilage, and IGF-binding proteins (IGFBPs) are an integral part of IGF axis, they may play role pathophysiology osteoarthritis. Chondrocytes isolated from fibrillated normal appearing areas osteoarthritic human were studied IGFBP expression. messenger ribonucleic acids analyzed by RT-quantitative PCR technique Northern...
<h3>Objective</h3> Interleukin (IL)-7 is mainly produced in bone marrow (BM) that forms the niche for B cells. We previously demonstrated BM also retains pathogenic memory CD4 T cells murine models of inflammatory bowel disease (IBD). However, it remains unknown whether BM-derived IL-7 sufficient development IBD and which form colitogenic BM. <h3>Design</h3> To address these questions, we developed mice expression was specific BM, identified colitis-associated IL-7-expressing mesenchymal...
Patients with ulcerative colitis [UC] are at an increased risk of developing colitis-associated cancer [CAC], suggesting that continuous inflammation in the colon promotes transformation colonic epithelial cells. However, mechanisms underlying cell UC remain unknown. We therefore aimed to investigate effect long-term on intestinal cells [IECs] using organoid culture. IECs were isolated from mouse colon, and cultured according a method for three-dimensional [3D] To mimic chronic inflammation,...
ABSTRACT Background Three Janus kinase (JAK) inhibitors are approved for ulcerative colitis (UC) in Japan. Aim To compare the real‐world efficacy and safety of these three JAK UC. Methods This was a multicentre, retrospective study patients with UC started on inhibitors. The primary outcome clinical remission at 10, 26 58 weeks, most recent follow‐up. among inhibitors, we created matched cohorts (upadacitinib vs. filgotinib, tofacitinib filgotinib upadacitinib tofacitinib) using propensity...
Endoscopic phenotypes of pouchitis according to the Chicago Classification have been reported be associated with poor pouch outcomes in ulcerative colitis (UC). Here, we aimed assess prevalence endoscopic and their predictability for outcomes. This retrospective multicenter study included UC patients aged 18 years or older who underwent total colectomy between January 2000 March 2020. The primary endpoints were frequencies chronic failure. findings evaluated at initial pouchoscopy 3 10 after...