Abstract Ghrelin, also called “the hunger hormone”, is a gastric peptide hormone that regulates food intake, body weight, as well taste sensation, reward, cognition, learning and memory. One unique feature of ghrelin its acylation, primarily with an octanoic acid, which essential for binding activation the receptor, G protein-coupled receptor. The multifaceted roles make receptor highly attractive drug target growth retardation, obesity, metabolic disorders. Here we present two cryo-electron...

Oxidative stress is closely associated with aging and age-related neurodegenerative diseases plays a vital function in their initiation progression. Selenium-enriched peptides from Cardamine violifolia (CSP) had excellent antioxidant vitro, however, whether it has an effect on still unknown. In this study, the results revealed that CSP supplementation could ameliorate d-galactose (d-gal) induced metabolism disorder, memory dysfunction, neuron damage hippocampus. Moreover, alleviated ROS MDA...

Ventilator-induced lung injury (VILI) during mechanical ventilation (MV) has been attributed to airway remodeling involving increased smooth muscle cells (ASMCs), but the underlying mechanism is not fully understood. Thus, we aimed investigate whether MV-associated high stretch (>10% strain) could modulate mechanosensitive Piezo1 expression and thereby alter cell migration of ASMCs as a potential pathway in VILI. C57BL/6 mice were subjected MV at tidal volume (VT, 18 mL/kg, 3 h) (13% strain,...

Selenium-enriched peptides from Cardamine violifolia (CSP) have excellent antioxidant functions but little is known about their effects on obesity and associated metabolic disorders in mice fed with a high-fat diet (HFD). In this study, C57BL/6 were HFD or without CSP supplementation (CSPL: 26 μg Se per kg bw d; CSPH: 104 d) for 10 weeks. The results showed that both CSPL CSPH could ameliorate overweight gain, excess fat accumulation, serum lipid metabolism, insulin resistance. potential...

Ventilator-induced lung injury (VILI) occurs in mechanically ventilated patients of respiratory disease and is typically characterized by airway inflammation. However, recent studies increasingly indicate that a major cause VILI may be the excessive mechanical loading such as high stretch (>10% strain) on smooth muscle cells (ASMCs) due to ventilation (MV). Although ASMCs are primary mechanosensitive airways contribute various inflammation diseases, it still unclear how they respond what...

Abstract Asthma is a chronic respiratory disease with high heterogeneity in human. Different mouse models have been applied for investigation of pathogenesis and treatment asthma, which target on different cells, receptors pathways. Interleukin (IL-) 28B, member λ-interferons, shown to play protective role OVA-induced antigen-specific adaptive immune system dominant. However, the roles IL-28B protease-induced an independent are still unclear. Here, we used plant-derived cysteine protease,...

Mechanical stretch is one type of common physiological activities such as during heart beating, lung breathing, blood flow through the vessels, and physical exercise. The mechanical stimulations regulate cellular functions maintain body homeostasis. It still remains to further characterize mechanical-biomechanical coupling mechanism. Here we applied fluorescence resonance energy transfer (FRET) technology visualize ERK activity in airway smooth muscle (ASM) cells under cyclic stimulation...

Objective: High stretch (strain >10%) can alter the biomechanical behaviors of airway smooth muscle cells which may play important roles in diverse lung diseases such as asthma and ventilator-induced injury. However, underlying modulation mechanisms for high stretch-induced mechanobiological responses ASMCs are not fully understood. Here, we hypothesize that respond to with increased expression specific microRNAs (miRNAs) turn modulate cells. Thus, this study aimed identify miRNA cultured is...

Inspired by the well-known phenomenon of stretch-induced airway dilation in normal lungs and emerging stretch-responsive Piezo1 channels that can be chemically activated specific agonists such as Yoda1, we attempted to investigate whether chemical activation Yoda1 modulate biomechanical behaviors smooth muscle cells (ASMCs) so it may exploited a novel approach for bronchodilation. Thus, treated vitro cultured rat ASMCs with examined calcium signaling, cell stiffness, traction force,...

Corneal blindness is a common phenomenon, and corneal transplantation an effective treatment for defects. However, there usually mismatch between the repair material degree of patient's defect. Therefore, patients with different defects need suitable materials specific microstructure personalized treatment. In this research, collagen films bionic structures were fabricated through ethanol evaporation technique by regulating volume ratios solution: = 10:0(Col)/9:1(CC91)/8:2(CC82)/CC73(CC73)....

Traditional herbal therapy made from Lycium barbarum leaves has been said to be effective in treating metabolic diseases, while its exact processes are yet unknown. Natural flavonoids considered as a secure and reliable method for obesity. We thus an effort investigate the by which L. (LBLF) reduce To assess effectiveness of intervention following intragastric injection various dosages LBLF (50, 100, 200 mg/kg⋅bw), obese model mice developed via high-fat diet were utilized. Treatment may...

Cell-cell mechanical communications at a large spatial scale (above hundreds of micrometers) have been increasingly recognized in recent decade, which shows importance tissue-level assembly and morphodynamics. The involved mechanosensing mechanism resulted physiological functions are still to be fully understood. Recent work showed that traction force sensation the matrix induces cell for self-assembly. Here, based on experimental model directional migration Matrigel hydrogel, containing 0.5...

Airway smooth muscle cells (ASMCs) exist in a form of helical winding bundles within the bronchial airway wall. Such tubular tissue provides with considerable curvature as physical constraint, which is widely thought an important determinant cell behaviors. However, this process difficult to mimic conventional planar culture system. Here, we report method develop chips cell-scale (concave and convex) surfaces from fused deposition modeling 3D printing explore how ASMCs adapt cylindrical for...

Abstract Ghrelin, also called “the hunger hormone”, is a gastric peptide hormone that regulates food intake, body weight, as well taste sensation, reward cognition, learning and memory. One unique feature of ghrelin its acylation, primarily with an octanoic acid, which essential for binding activation the receptor, G protein-coupled receptor. The multifaceted roles make receptor highly attractive drug target growth retardation, obesity, metabolic disorders. Here we present two cryo-electron...

Corneal defects can seriously affect human vision, and keratoplasty is the most widely accepted therapy method for visual rehabilitation. Currently, effective treatment clinical patients has been restricted due to a serious shortage of donated cornea tissue high-quality artificial repair materials. As predominant component tissue, collagen-based materials have promising applications corneal repair. However, nerve epithelization process after transplantation must be improved. This research...

High stretch (>10% strain) of airway smooth muscle cells (ASMCs) due to mechanical ventilation (MV) is postulated contribute ventilator-induced lung injury (VILI), but the underlying mechanisms remain largely unknown. We hypothesized that ASMCs may respond high via regulatory miRNA–mRNA interactions, and thus we aimed identify stretch-responsive cellular events related regulating interactions in cultured human with/without stretch. RNA-Seq analysis whole genome-wide miRNAs revealed 12...

α-Alkyl and α-aryl vinyl azides were found to be able couple with cysteine-derived alkyl thiols chemoselectively under mild conditions, providing the corresponding β-ketosulfides simultaneous extrusion of N 2 ammonia.

Objective: The Coronavirus Disease 2019 (COVID-19) has become a global pandemic, causing millions of people to be infected worldwide Imaging analysis based on computed tomography (CT) data is an important means clinical diagnosis A supercomputing-supported method proposed for the construction new comprehensive CT auxiliary system dealing with pneumonia Method: consists four parts: input processing module, preprocessing imaging subsystem, and artificial intelligence(AI) subsystem Among parts,...

<title>Abstract</title> Background Neural cell adhesion molecule (NCAM), a common mammalian surface glycoprotein, is the major substrate of polysialic acid (PSA). Polysialylated NCAM occurs in many types cancer, but rarely normal adult tissues. Epithelial-mesenchymal transition (EMT) an important process contributing to tumor metastasis. The functional role hypersialylation EMT unclear. Method Expression and PSA-NCAM breast cancer progression were evaluated by western blot (WB),...