A5033487562- Orthopaedic implants and arthroplasty
- Hip and Femur Fractures
- Total Knee Arthroplasty Outcomes
- Hip disorders and treatments
- Immune Response and Inflammation
- Orthopedic Infections and Treatments
- interferon and immune responses
- Traditional Chinese Medicine Studies
- Pelvic and Acetabular Injuries
- Bone fractures and treatments
- Pharmacological Effects of Natural Compounds
- Gut microbiota and health
- Phytochemistry and Bioactive Compounds
- Immune Cell Function and Interaction
- Systemic Lupus Erythematosus Research
- Marine Sponges and Natural Products
- Essential Oils and Antimicrobial Activity
- T-cell and Retrovirus Studies
- Viral-associated cancers and disorders
- Phytochemicals and Medicinal Plants
- Dielectric materials and actuators
- Biological and pharmacological studies of plants
- Helicobacter pylori-related gastroenterology studies
- Chromatography in Natural Products
- Pharmacological Effects of Medicinal Plants
Zhejiang Chinese Medical University
2008-2025
First Affiliated Hospital of Soochow University
2008-2024
Soochow University
2008-2024
Shanghai Institute of Applied Physics
2001-2002
Chinese Academy of Sciences
2001
Abstract Current extraction methods often extract DNA and RNA separately, few are capable of co-extracting from sputum. We established a nucleic acid co-extraction method sputum based on magnetic beads optimized the by evaluating influencing factors, such as guanidinium thiocyanate (GTC) dithiothreitol (DTT) concentrations, bead amount, incubation temperature, lysis buffer pH carrier type. The feasibility simultaneous was evaluated amplifying viruses single clinical specimen with multiplex...
l-Lactic acid (l-LA) is a platform chemical obtained via microbial fermentation at near-neutral pH value. Large amounts of neutralizers are required during this process, which increases the production costs in downstream processing as well environmental burden. To address challenge, an acid-tolerant yeast Pichia kudriavzevii E1 was isolated and metabolically engineered to produce l-LA without neutralizers. The genome strain sequenced CRISPR-Cas9 system developed newly strain. Subsequently,...
Jieduquyuziyin prescription (JP) has been used to treat systemic lupus erythematosus (SLE). Although the effectiveness of JP in treatment SLE clinically proven, underlying mechanisms have yet be completely understood. We observed therapeutic actions MRL/lpr mice and their bone marrow-derived macrophages (BMDMs) potential mechanism inhibition inflammatory activity. To estimate effect on suppressing activity, BMDMs MRL/MP were treated with JP-treated serum, by for eight weeks. Among them, its...
Wear debris-induced osteolysis and ensuing aseptic loosening is the main cause of implant failure revision surgery. inflammatory response plays key roles in peri-implant osteolysis. Recently, substantial evidence suggests that hydrogen sulfide (H2 S), third gasotransmitter, a critical player regulating inflammation. However, role therapeutic potential H2 S wear inflammation remains to be defined. In present study, we investigated effect on pro-inflammatory cytokines expression vitro vivo....
In this work, a headspace system coupled to gas chromatographic column and ion mobility spectrometry was applied as screening differentiate the crude processed "atractylodis macrocephalae rhizoma" samples. The obtained data were consecutively by principal component analysis Radar fingerprint chart methods. From for initial solution original variables, two components accounted 68 13% of total variance, respectively. established method proven be valuable classification, discrimination between...
Abstract Background The association between gut microbiota and ankylosing spondylitis (AS) has been reported in the literature; however, whether two are correlative is unclear. Methods Single nucleotide polymorphisms associated with microbiome composition AS (968 cases 336 191 controls) were obtained from published genome‐wide studies this two‐sample Mendelian randomization (MR) study. causal relationship was estimated using inverse‐variance weighted method, robustness of our findings...
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease, and Jieduquyuziyin prescription (JP) traditional Chinese medicine (TCM) formula that has been testified to be effective for SLE treatment as an approved hospital many years in China. However, its mechanism of action the this disease largely unknown. The purpose study was determine whether JP-treated rat serum can inhibit activation peritoneal macrophages MRL/lpr mice by downregulating IRAK1 signaling pathway, thereby...
Abstract Two new clerodane‐based furanoid diterpenoids, tinosagittones A and B ( 1 2 , resp.), were isolated from the roots of Tinospora sagittata together with five known i.e. columbin 3 ), its glucoside palmatoside C 4 isocolumbin 5 6‐hydroxycolumbin 6 tinophylloloside 7 ). Their structures established by mass spectrometry spectroscopic methods, especially 2D‐NMR techniques.
Objective The purpose of this study was to present the surgical technique and clinical results percutaneous compression plate ( PCCP ) for treatment femoral neck fractures. Methods Between D ecember 2010 A pril 2013, 74 consecutive patients with fractures were treated by closed reduction implants in our university hospital. Their mean age 51.3 years (range, 15–83 years); 38 (51.4%) male 46 (62.2%) on left. patients' radiographic data analyzed retrospectively. Results There 45 undisplaced...
Abstract Two new polyketides, neosarphenols A and B ( 1 2 , resp.), were isolated from Neosartorya glabra together with six known 3 – 8 two meroterpenoids, 9 10 . The structures of the compounds elucidated by comprehensive spectroscopic analysis, especially HR‐ESI‐MS NMR experiments. All evaluated for their cytotoxic activities against MDA‐MB‐231, MCF‐7, PANC‐1 tumor cell lines; 6 exhibited selective moderate cytotoxicities line.
The adjusted mechanical alignment (aMA) technique is an extension of conventional (MA), which has rarely been reported. purpose this study was to evaluate mid-term outcomes navigation-assisted total knee arthroplasty (TKA) using aMA.This retrospective cohort enrolled 63 consecutive patients (77 knees) who underwent TKA aMA between September 2017 and October 2019. Fifty-two (61 MA during the same period were assessed as controlled group. demographic data perioperative recorded. parameters...
Abstract The resistivity of different polymer composites is investigated in temperature‐changing processes, which demonstrates more complicated changing behaviors than that usually reported literatures. resistivity‐temperature (R‐T) cannot be explained by current theories, and the contradictions them with theories are presented this article. With experiments, a new viewpoint on R‐T proposed, i.e., changes attributed to internal stress generated when morphology structure undergo great...
Abstract Objective To describe a new alignment technique of adjusted restricted kinematic (arKA) for the treatment severe varus deformity in total knee arthroplasty. Methods Three female patients (three knees) who underwent navigation‐assisted arthroplasty (TKA) using arKA from April 2020 to September were included this study, with an average age 71.33 years (range, 61 80 years). General anesthesia was given all patients. Intraoperative observations including tibia resection angle, frontal...
Innate immune memory can cause the occurrence and exacerbation of autoimmune diseases, it is as well being strongly associated with pathogenesis systemic lupus erythematosus (SLE), however, specific mechanism remains to be further studied. We learned that IFN-γ stimulation generated innate in bone marrow-derived macrophages (BMDMs) activated interferon-stimulated genes (ISGs). This research used lipopolysaccharide (LPS) treat BMDMs lupus-prone MRL/lpr mice showed particular ISGs were...
Genetic and environmental elements contribute to the incidence of systemic lupus erythematosus (SLE). In this study, we aimed investigate role EBV latent membrane protein 1 (LMP-1) in regulation susceptibility gene expression SLE. RNA-seq data from peripheral blood SLE patients H9 T cells were used. LMP-1 ARID5B up-regulated overexpression increased level ARID5B. Histone H3K27m enhancer zeste homolog 2 (EZH2), which catalyzes methylation histone H3K27m, also significantly increased....
The association between gut microbiota (GM) and breast cancer (BC) has been studied. Nevertheless, the causal relationship them potential mediating factors have not clearly defined. Therefore, in this study, Mendelian randomization analysis (MR) was employed to explore 473 GM BC, as well effect of immune cells. In investigation, we availed ourselves publicly accessible summary statistics from genome-wide study undertake two-sample reverse analyses on with intention clarifying BC....