A5103164205- Prostate Cancer Treatment and Research
- Glioma Diagnosis and Treatment
- Prostate Cancer Diagnosis and Treatment
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- PI3K/AKT/mTOR signaling in cancer
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Legionella and Acanthamoeba research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ferroptosis and cancer prognosis
- Radiomics and Machine Learning in Medical Imaging
- Cancer, Lipids, and Metabolism
- Economic and Financial Impacts of Cancer
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Single-cell and spatial transcriptomics
- Vibrio bacteria research studies
- Anodic Oxide Films and Nanostructures
- Thyroid Disorders and Treatments
- Water Treatment and Disinfection
- Cancer-related cognitive impairment studies
- Inflammatory mediators and NSAID effects
- Primary Care and Health Outcomes
University of Chicago
2025
SUNY Upstate Medical University
2025
Hospital for Sick Children
2020-2024
SickKids Foundation
2020-2024
Queen's University
2016-2023
University of Southern California
2018-2023
MedStar Georgetown University Hospital
2021
Georgetown University
2021
University of Manitoba
2013-2021
NYU Langone Health
2019
Cancer biomarker studies often require nucleic acid extraction from limited amounts of formalin-fixed, paraffin-embedded (FFPE) tissues, such as histologic sections or needle cores. A major challenge is low quantity and quality extracted acids, which can limit our ability to perform genetic analyses, have a significant influence on overall study design. This was aimed at identifying the most reliable reproducible method obtaining sufficient high-quality acids FFPE tissues. We compared yield...
Abstract Histone H3 lysine 27 (H3K27M) mutations represent the canonical oncohistone, occurring frequently in midline gliomas but also identified haematopoietic malignancies and carcinomas. H3K27M functions, at least part, through widespread changes H3K27 trimethylation its role tumour initiation remains obscure. To address this, we created a transgenic mouse expressing H3.3K27M diverse progenitor cell populations. expression drives tumorigenesis multiple tissues, which is further enhanced...
// Stephanie A. Toll 1, 2, * , Hung N. Tran 3, Jennifer Cotter 4 Alexander R. Judkins Benita Tamrazi 5 Jaclyn Biegel Girish Dhall 2 Nathan J. Robison Kaaren Waters 1 Palak Patel Robert Cooper and Ashley S. Margol Division of Hematology, Oncology Blood Marrow Transplantation, Children's Center for Cancer Diseases, Hospital Los Angeles, CA, USA University Southern California Keck School Medicine, 3 Kaiser Permanente California, Department Pathology Laboratory Radiology Imaging, These authors...
Abstract High-grade diffuse glioma (HGG) is the leading cause of brain tumour death. While genetic drivers HGG have been well described, targeting these has thus far had little impact on survival suggesting other mechanisms are at play. Here we interrogate alternative splicing landscape pediatric and adult through multi-omic analyses, uncovering an increased burden compared with normal brain. The rate recurrent in cancer exceeds their mutation rate, a pattern that recapitulated pan-cancer...
Abstract Background Education in infectious disease (ID) is a critical part of an internal medicine curriculum. As the consult service typically high volume and busy, residents other trainees were often not receiving adequate training less common conditions or didactic that may be relevant for their future practice board exams. Our Quality Improvement project sought to improve medical students through integration high-yield lecture services given on Adult ID service.Figure 1:Pre/Post Test...
Abstract Background Deep surgical site infection after vascular surgery is a dreaded complication. In October 2023 surgeon raised concerns about an increase in post-operative Pseudomonas infections with the prevention and control team at University of Chicago Medicine. Methods Medicine 800 bed tertiary academic medical center active practice. Investigation suspected cluster was conducted by Infection Prevention Control (IPC) included molecular analysis clinical isolates, environmental...
260 Background: Inactivation of tumor suppressor gene, PTEN (Phosphatase and tensin homolog) by deletion or mutation occurs in between 15-20% localized prostate cancers up to 50% castration-resistant cancers. The series studies has shown a robust correlation the genomic loss its protein as assessed immunohistochemistry (IHC). Dysregulation PI3K/AKT/mTOR signaling pathway been linked with adverse pathological features poor oncological outcomes such development disease recurrence, metastasis,...
Formalin-fixed paraffin embedded tissue (FFPET) represents a valuable, well-annotated substrate for molecular investigations. The utility of FFPET in analysis is complicated both by heterogeneous composition and low yields when extracting nucleic acids. A literature search revealed paucity protocols addressing these issues, none that showed validated method simultaneous extraction RNA DNA from regions interest FFPET. This addresses issues. Tissue specificity was achieved mapping cancer areas...
Background We identify and validate accurate diagnostic biomarkers for prostate cancer through a systematic evaluation of DNA methylation alterations. Materials methods assembled three early cohorts (total patients = 699) from which we collected processed over 1300 prostatectomy tissue samples extraction. Using real‐time methylation‐specific PCR, measured normalized levels at 15 frequently methylated loci. After partitioning sample sets into independent training validation cohorts,...
Phosphatase and tensin homolog (PTEN) loss has long been associated with adverse findings in early prostate cancer. Studies to date have yet employ quantitative methods (qPTEN) for measuring of prognostically relevant amounts PTEN postsurgical settings demonstrate its clinical application.PTEN protein levels were measured by immunohistochemistry radical prostatectomy samples from training (n = 410) validation 272) cohorts. was quantified per cancer cell tissue microarray core. Thresholds...
Legionella pneumophila, an intracellular parasite of protozoa, possesses a distinct dimorphic life cycle that alternates between the vegetative replicative form and resilient but highly infectious cyst form. Previously, temporally expressed heterodimeric integration host factor (IHF) was shown to be required for differentiation into However, precise regulatory mechanisms controlling expression IHF have not been identified. Microplate kinetic assays with GFP reporter promoter fusion...
Abstract Mating and transfer of male sex peptide (SP), or transgenic expression SP, causes inflammation decreased life span in female Drosophila. Mifepristone rescues these effects, yielding dramatic increases span. Here targeted metabolomics data were integrated with further analysis extant transcriptomic data. Each 7 genes positively correlated expressed the brain eye involved regulation gene signaling. Genes negatively preferentially midgut protein degradation, amino acid metabolism,...
ABSTRACT Nominally an environmental organism, Legionella pneumophila is intracellular parasite of protozoa but also the causative agent pneumonia termed Legionnaires' disease, which results from inhalation aerosolized bacteria by susceptible humans. Coordination gene expression a number identified regulatory factors, including OxyR, assists L. in adapting to stresses changing environments. OxyR (OxyR Lp ) ortholog Escherichia coli OxyR; however, was shown elsewhere be functionally divergent,...
8507 Background: Appearance of auto-AB during IFN-adjuvant therapy is a prognostic factor in melanoma pts. We evaluated this the EORTC 18952 trial including pts without IFN. Methods: Anti-cardiolipin (C) anti-thyreoglobulin (T) and anti- nuclear (N) AB levels serial samples between 0 48 months (mt) were assessed centrally. Prognostic impact CTN on relapse-free survival (RFS) was by 3 methods: 1) usual Cox model (lead-time bias); 2) Time dependent using latest positive value; 3) any value....
Formalin-fixed paraffin embedded tissue (FFPET) represents a valuable, well-annotated substrate for molecular investigations. The utility of FFPET in analysis is complicated both by heterogeneous composition and low yields when extracting nucleic acids. A literature search revealed paucity protocols addressing these issues, none that showed validated method simultaneous extraction RNA DNA from regions interest FFPET. This addresses issues. Tissue specificity was achieved mapping cancer areas...
Abstract BACKGROUND Diffuse Midline Glioma (DMG) is a incurable tumor affecting children. Recent genomic investigations have identified recurrent H3K27M mutation which induces global alterations in histone methylation patterns and DNA methylation. These epigenetic modifications hint at pivotal roles DMG pathogenesis, yet effective therapeutic strategies remain elusive, with median survival rates stagnant approximately one year. This shortfall stems from two main factors: 1) Inadequate...
Abstract Legionella pneumophila possesses a unique intracellular lifecycle featuring distinct morphological stages that include replicative forms and transmissive cyst forms. Expression of genes associated with virulence traits morphogenesis is concomitant, governed by complex stringent response based‐regulatory network the stationary phase sigma factor RpoS. In Pseudomonas spp., rpoS expression controlled autorepressor PsrA, orthologs PsrA RpoS are required for formation in Azotobacter ....