A5102927870- Metabolomics and Mass Spectrometry Studies
- Genomics, phytochemicals, and oxidative stress
- Diet, Metabolism, and Disease
- Lipid Membrane Structure and Behavior
- Escherichia coli research studies
- Carbohydrate Chemistry and Synthesis
- Force Microscopy Techniques and Applications
- Polymer Surface Interaction Studies
- Cancer Genomics and Diagnostics
- Microbial Inactivation Methods
- Free Radicals and Antioxidants
- Mass Spectrometry Techniques and Applications
- Acute Myeloid Leukemia Research
- Garlic and Onion Studies
- Single-cell and spatial transcriptomics
- Advanced Glycation End Products research
- Analytical Chemistry and Chromatography
- Cellular Mechanics and Interactions
- Viral gastroenteritis research and epidemiology
- Biochemical Analysis and Sensing Techniques
- Cancer Cells and Metastasis
- Agronomic Practices and Intercropping Systems
- Hydrogels: synthesis, properties, applications
- Salmonella and Campylobacter epidemiology
- Advanced Thermodynamics and Statistical Mechanics
Robert Bosch Hospital
2021-2025
Technische Universität Berlin
1993-2024
Queen's University
2014-2024
Kingston Health Sciences Centre
2023
Berliner Wasserbetriebe (Germany)
2021
Leibniz-Institute for Food Systems Biology at the Technical University of Munich
2018
Roche Pharma AG (Germany)
2015-2017
University of Vienna
2017
Leibniz Institute of Polymer Research
2009-2012
Kliniken Essen-Mitte
2011
Clinical research relies on high-quality patient data, however, obtaining big data sets is costly and access to existing often hindered by privacy regulatory concerns. Synthetic generation holds the promise of effectively bypassing these boundaries allowing for simplified accessibility prospect synthetic control cohorts. We employed two different methodologies generative artificial intelligence - CTAB-GAN+ normalizing flows (NFlow) synthesize derived from 1606 patients with acute myeloid...
The biological activity of 24R,25-dihydroxyvitamin D3 [24R,25(OH)2D3] remains controversial, but it has been suggested that contributes to fracture healing. Cyp24a1–/– mice, synthesizing no 24R,25(OH)2D3, show suboptimal endochondral ossification during repair, with smaller callus and reduced stiffness. These defects were corrected by 24R,25(OH)2D3 treatment, not 1,25-dihydroxyvitamin D3. Microarrays mRNA identified FAM57B2 as a mediator the effect. produced lactosylceramide (LacCer) upon...
The authors report on the characterization of charge formation at supported bilayer lipid membranes (sBLMs) prepared from zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine planar silicon dioxide substrates. charging sBLMs was studied in KCl solutions different ionic strengths between 0.1 and 10 mM by streaming current measurements. In addition, attenuated total reflection Fourier transform infrared spectroscopy fluorescence microscopy were applied to determine concentration membrane...
Rocket is rich in glucosinolates and valued for its hot spicy taste. Here we report the structure elucidation, bioactivity, stability of mainly formed glucosinolate hydrolysis product, namely sativin, which was formerly thought to be 4-mercaptobutyl isothiocyanate. However, by NMR characterization revealed that sativin fact 1,3-thiazepane-2-thione, a tautomer isothiocyanate with 7-membered ring so far unknown. This finding further substantiated conformation sampling using molecular modeling...
The tyrosine-protein phosphatase nonreceptor type 11 (PTPN11) is an important regulator of RAS signaling and frequently affected by mutations in patients with acute myeloid leukemia (AML). Despite the relevance for leukemogenesis as a potential therapeutic target, prognostic role controversial. To investigate impact PTPN11 mutations, we analyzed 1529 adult AML using next-generation sequencing. were detected 106 (6.93%) (median VAF: 24%) dominant (36%) subclonal (64%) configuration. Patients...
Red cabbage is a popular vegetable in Central Europe and rich source of glucosinolates (GLSs). Upon hydrolysis, GLSs form health-promoting isothiocyanates (ITCs), but also nitriles epithionitriles. Recently, ITCs were shown to undergo further yielding amines. Here, we analyzed the degradation formation ITCs, nitriles, epithionitriles, amines during domestic-like cooking red with addition vinegar or baking soda. Both additives strongly affected stability boiling. Primary found as major...
Glucosinolate-derived isothiocyanates are valuable for human health as they exert promoting effects. As thermal food processing could affect their levels in a structure dependent way, the stability and reactivity of 12 Brassicaceae during aqueous heating at 100 °C pH 5-8 were investigated. The formation corresponding amines N,N'-dialk(en)yl thioureas was quantified. Further, potential to form odor active compounds investigated by HRGC-MS-olfactometry. A strong structure-reactivity...
Fluidity and charging of supported bilayer lipid membranes (sBLMs) prepared from 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) were studied by fluorescence recovery after photobleaching (FRAP) microslit electrokinetic measurements at varying pH ionic composition the electrolyte. Measurements in neutral electrolytes (KCl, NaCl) revealed a strong correlation between membrane fluidity due to unsymmetrical water ion adsorption (OH(-) ≫ H(3)O(+)). The significantly decreased below...
Colorectal cancer (CRC) is the second leading cause of deaths. Despite recent advances, five-year survival rates remain largely unchanged. Desorption electrospray ionization mass spectrometry imaging (DESI) an emerging nondestructive metabolomics-based method that retains spatial orientation small-molecule profiles on tissue sections, which may be validated by 'gold standard' histopathology. In this study, CRC samples were analyzed DESI from 10 patients undergoing surgery at Kingston Health...
We report on thermo- and pH-responsive copolymer films consisting of N-isopropylacrylamide (NIPAAm) different carboxyalkylacrylamides exhibiting switchable swelling states over a wide range temperatures pH-values. The copolymers were covalently grafted as brush-like onto solid substrates. Analysis by quartz crystal microbalance with dissipation monitoring revealed the phase transition behaviour to be sensitively controlled balance polar non-polar interactions, which could dissected into...
Ambient mass spectrometry (MS) technologies have been applied to spatial metabolomic profiling of various samples in an attempt both increase analysis speed and reduce the length sample preparation. Recent studies, however, focused on improving resolution ambient approaches. Finer requires greater times commensurate computing power for more sophisticated data algorithms larger sets. Higher provides a detailed molecular picture sample; some applications, this is not required. A liquid...
Polymer-cushioned lipid bilayers are frequently used to mimic the native environment of cellular membranes in respect extracellular matrix and intracellular structures. With aim actively tune membrane characteristics, we pursue approach use temperature pH responsive polymer thin films poly(N-isopropylacrylamide-co-carboxyacrylamide) (PNIPAAm-co-carboxyAAM) as cushions for supported bilayers. A cationic bilayer composed dioleoylphosphatidylcholine (DOPC) dioleoyltrimethylammoniumpropane...
In this study, α-dicarbonyl compounds consisting of a backbone with six carbon atoms resulting from the Maillard reaction d-fructose γ-aminobutyric acid were determined. The was carried out under mild conditions at 50 °C and water contents between 0 90%. A thus far unknown compound found as main product in first 24 h below 50%. After isolation its stable quinoxaline derivative, it possible to identify 2-deoxy-d- glycero-hexo-3,4-diulose (2-deoxyglucosone). For time, four C6-α-dicarbonyl...