A5101608900- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Hematopoietic Stem Cell Transplantation
- Tissue Engineering and Regenerative Medicine
- Cardiac Fibrosis and Remodeling
- Diabetes and associated disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- CAR-T cell therapy research
- Evolutionary Game Theory and Cooperation
- Mesenchymal stem cell research
- Gene Regulatory Network Analysis
- Bacterial biofilms and quorum sensing
- Biomedical and Engineering Education
- Immune responses and vaccinations
- Prostate Cancer Treatment and Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cardiac Structural Anomalies and Repair
- Vibrio bacteria research studies
- Memory and Neural Mechanisms
- Single-cell and spatial transcriptomics
- Neuroscience and Neuropharmacology Research
- Endoplasmic Reticulum Stress and Disease
- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
Inserm
2007-2023
Centre National de la Recherche Scientifique
2014-2023
Structure fédérative de recherche Necker
2014-2023
Délégation Paris 5
2007-2018
Université Paris Cité
2007-2018
Centre de Gestion Scientifique
2016
Sorbonne Paris Cité
2014
Institut Necker Enfants Malades
2002-2007
Hôpital Necker-Enfants Malades
2007
Institute for Economic Research
2005
Bone regeneration relies on the activation of skeletal stem cells (SSCs) that still remain poorly characterized. Here, we show periosteum contains SSCs with high bone regenerative potential compared to marrow stromal cells/skeletal (BMSCs) in mice. Although periosteal (PCs) and BMSCs are derived from a common embryonic mesenchymal lineage, postnatally PCs exhibit greater clonogenicity, growth differentiation capacity than BMSCs. During repair, can efficiently contribute cartilage bone,...
T cell–dependent immune responses develop soon after birth, whereas it takes 2 yr for humans to cell–independent responses. We used this dissociation analyze the repertoire diversification of IgM+IgD+CD27+ B cells (also known as “IgM memory” cells), comparing these with switched in children <2 age, aim determining whether two subsets are developmentally related. show that spleen and blood displays no sign antigen-driven activation expansion on H-CDR3 spectratyping, despite many...
Maturation of functional neuronal circuits during central nervous system development relies on sophisticated mechanisms. First, axonal and dendritic growth should reach appropriate targets for correct synapse elaboration. Second, pruning death are required to eliminate redundant or inappropriate connections. Serotonin, in addition its role as a neurotransmitter, actively participates postnatal establishment refinement brain wiring mammals. Brain resident macrophages, that is, microglia, also...
Regulatory T cells (Treg) are commonly identified by CD25 (IL-2R alpha) surface expression and/or intracellular of the FOXP3 transcription factor. In addition, Treg also characterized low CD127 (IL-7R when compared to conventional and their biology in periphery is considered essentially independent IL-7. We further investigated on we demonstrated differential depending subsets considered. Notably, observed high inducible costimulatory molecule (ICOS)- CD103-expressing subsets. Since these...
Identification of a thymus-seeding progenitor originating from human bone marrow (BM) constitutes key milestone in understanding the mechanisms T cell development and provides new potential for correcting deficiencies. We report characterization novel lymphoid-restricted subset, which is part lineage-negative CD34+CD10+ population distinct B cell–committed precursors (in view absence CD24 expression). demonstrate that these Lin−CD34+CD10+CD24− progenitors have very low myeloid but can...
X-linked chronic granulomatous disease (CGD) is associated with defective phagocytosis, life-threatening infections, and inflammatory complications. We performed a clinical trial of lentivirus-based gene therapy in four patients (NCT02757911). Two show stable engraftment benefits, whereas the other two have progressively lost gene-corrected cells. Single-cell transcriptomic analysis reveals significantly lower frequency hematopoietic stem cells (HSCs) CGD patients, especially engraftment....
Myotonic dystrophy type 1 is a neuromuscular affection associated with the expansion of an unstable CTG repeat in DM protein kinase gene. The disease characterized by somatic tissue-specific mosaicism and very high intergenerational instability strong bias towards expansions. We used transgenic mice carrying more than 300 repeats within their large human genomic environment to investigate dynamics germinal males. Germinal expansions was already present spermatozoa at 7 weeks age continued...
Abstract CD4+CD25+ regulatory T cells have been extensively studied during the last decade, but how these exert their function on pathogenic effector remains to be elucidated. Naive CD4+ transferred into cell-deficient mice strongly expand and rapidly induce inflammatory bowel disease (IBD). Onset of this disorder depends IFN-γ production by expanding cells. Coinjection protects recipient from IBD. In study, we show that do not affect initial activation/proliferation injected naive as well...
Abstract We characterized CD8+ T cells constitutively expressing CD25 in mice lacking the expression of MHC class II molecules. showed that these are present not only periphery but also thymus. Like CD4+CD25+ cells, CD8+CD25+ appear late during ontogeny. Peripheral from II-deficient share phenotypic and functional features with regulatory cells: particular, they strongly express glucocorticoid-induced TNFR family-related gene, CTLA-4 Foxp3, produce IL-10, inhibit CD25− cell responses to...
CD25(+)CD4(+) regulatory T cells have major roles in controlling immune responses, and use heterogeneous mechanisms. It is possible that these different activities are mediated by subsets. Here we show CD103(+)CD25(+)CD4(+) (that control inflammatory bowel disease) highly enriched gut-associated lymphoid tissue unique functional properties. In vivo, only this subpopulation able to wasting disease peripheral cell homeostasis. vitro, regulate IL-10 secretion, it might also mediate infectious...
Abstract Natural regulatory T cells (Tregs) are present in high frequencies among tumor-infiltrating lymphocytes and draining lymph nodes, supposedly facilitating tumor development. To investigate their role controlling local immune responses, we analyzed intratumoral cell accumulation function the presence or absence of Tregs. Tumors that grew normal BALB/c mice injected with 4T1 line were highly infiltrated by Tregs, CD4 CD8 cells, all having unique characteristics. Most infiltrating Tregs...
The evolutionary success of bacteria depends greatly on their capacity to continually generate phenotypic diversity. Structured environments are particularly favorable for diversification because attenuated clonal interference, which renders selective sweeps nearly impossible and enhances opportunities adaptive radiation. We examined at the microscale level emergence spatial temporal dynamics diversity underlying causes in Escherichia coli colonies. An important dynamic heterogeneity growth,...
Dendritic cells (DC) have been reported to migrate in afferent lymph the steady state. However, it is unknown whether DC traffic modulated by nature of drained tissue. To analyze influence mucosal versus cutaneous microenvironments on constitutive release, we exploited a novel technique cannulation sheep, which allowed comparison migrating from head mucosae [cervical (CerDC)] with skin [prescapular (PresDC)]. The migration rate was lower for CerDC than PresDC. Compared PresDC, contained...
Abstract The role of the thymus in T cell commitment hemopoietic precursor is yet controversial. We previously identified a major progenitor activity cells isolated from bone marrow-derived spleen colonies. In this study, we characterize properties these pre-T cells. demonstrate that they have unique phenotype and can be generated total absence any thymic influence. Indeed, even when studied at single-cell level, extrathymic cell-committed precursors express cell-specific genes. Moreover,...
Common lymphoid progenitors (CLP) are generated in adult bone marrow (BM), but the intermediate steps leading to T cell commitment unknown, and so is site at which this occurs. Here, we show that colonies arising spleen 12 days after BM injection harbor precursors undetectable BM. These did not generate myeloid cells vivo repopulated thymus peripheral compartment much faster than CLP. Two lineage negative (Lin−) subpopulations were distinguished, namely CD44+ Thy1− still capable of natural...
We have developed a new method for accurate quantification of dead microbial cells. This technique employs the simultaneous use fluorescent hydrazides and nucleic acid dyes. Fluorescent allow detection cells that cannot be detected with currently used high-affinity is particularly important nongrowing bacterial populations multicellular communities containing physiologically heterogeneous cell populations, such as colonies biofilms.
Regulation of cell cycle in beta cells is poorly understood, especially humans. We exploited here the recently described human pancreatic line EndoC-βH2 to set up experimental systems for studies. derived 2 populations from that stably harbor genes encoding Fucci fluorescent indicators cycle, either two vectors, or a unique bicistronic vector. In proliferating non-synchronized cells, revealed expected phases with orange and green being G1 S/G2/M respectively, allowed sorting different...
Abstract Several extrathymic T cell progenitors have been described but their various contributions to the lineage puzzle are unclear. In this study, we provide evidence for a splenic Lin−Thy1.2+ cell-committed population, rare in B6 mice, abundant TCRα−/−, CD3ε−/−, and nude absent IL-7- Rag-2-deficient mice. Neither B nor myeloid cells generated vivo vitro. The incidence of these pre-T is under control thymus and/or mature cells, as revealed by graft experiments. Indeed, IL-7 consumption...
Accumulation of senescent dermal fibroblasts drives skin aging. The reactivation proliferation is one strategy to modulate cell senescence. Recently, we reported the exact chemical composition hydrophilic extract Oenothera biennis cultures (ObHEx) and showed its anti-aging properties. aim this work assess biological effect specifically on ObHEx action has been evaluated normal human subjected stress-induced premature senescence (SIPS) through an ultra-deep proteomic analysis, leading most...