A5101422966- Genomics and Phylogenetic Studies
- Cancer therapeutics and mechanisms
- Drug Transport and Resistance Mechanisms
- Enzyme Production and Characterization
- Circular RNAs in diseases
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- biodegradable polymer synthesis and properties
- MicroRNA in disease regulation
- NF-κB Signaling Pathways
- Cancer-related molecular mechanisms research
- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Epigenetics and DNA Methylation
- Antibiotics Pharmacokinetics and Efficacy
- Metabolism and Genetic Disorders
- Metabolism, Diabetes, and Cancer
- Diagnosis and treatment of tuberculosis
- Synthesis and biological activity
- Photodynamic Therapy Research Studies
- Mycobacterium research and diagnosis
- Enzyme Catalysis and Immobilization
- Pancreatic function and diabetes
- Escherichia coli research studies
- Extracellular vesicles in disease
Beijing Institute of Genomics
2017-2024
Chinese Academy of Sciences
2017-2024
Shaanxi Provincial People's Hospital
2022-2024
Shanxi Medical University
2022-2024
Zhejiang University of Technology
2019-2022
University of Chinese Academy of Sciences
2017-2021
Sichuan University
2020
Indiana University School of Medicine
2019
The University of Texas Southwestern Medical Center
2016
University of Manitoba
2013-2015
ABSTRACT Many studies have demonstrated that intravenously administered bacteria can target and proliferate in solid tumors then quickly be released from other organs. Here, we employed the tumor-targeting property of Escherichia coli Nissle 1917 to inhibit mouse B16 melanoma 4T1 breast through expression azurin protein. For this purpose, recombinant azurin-expressing E. was developed. The levels vitro vivo secretion engineered bacterium were determined by immunochemistry. Our results...
Tuberculosis now exceeds HIV as the top infectious disease cause of mortality, and is caused by Mycobacterium tuberculosis complex (MTBC). MTBC strains have highly conserved genome sequences (similarity > 99%) but dramatically different phenotypes. To analyze relationship between genotype phenotype, we conducted comparative genomic analysis on 12 representing lineages (i.e., bovis; M. bovis BCG; microti; africanum; H37Rv; H37Ra, six clinical isolates). The focused three aspects...
A novel strain of Pseudomonas putida LS46 was isolated from wastewater on the basis its ability to synthesize medium chain-length polyhydroxyalkanoates (mcl-PHAs). P.putida differentiated other strains cpn60 (UT). The complete genome sequenced and annotated. Its chromosome is 5,86,2556 bp in size with GC ratio 61.69. It encoding 5316 genes, including 7 rRNA genes 76 tRNA genes. Nucleotide sequence data P. compared nine (KT2440, F1, BIRD-1, S16, ND6, DOT-T1E, UW4, W619 GB-1) identified either...
Abstract Klebsiella pneumoniae (K. pneumoniae) is an important pathogen that can cause severe hospital- and community-acquired infections. To systematically investigate its methylation features, we determined the whole-genome sequences of 14 K. strains covering varying serotypes, multilocus sequence types, clonal groups, viscosity/virulence, drug resistance. Their methylomes were further characterized using Pacific Biosciences single-molecule real-time bisulfite technologies. We identified...
Thermoanaerobacter spp. have long been considered suitable Clostridium thermocellum coculture partners for improving lignocellulosic biofuel production through consolidated bioprocessing. However, studies using "omic"-based profiling to better understand carbon utilization and producing pathways limited only a few strains thus far. To characterize electron flux in the recently isolated, xylanolytic strain, thermohydrosulfuricus WC1, label-free quantitative proteomic analyses were combined...
Growing interest in cellulolytic clostridia with potential for consolidated biofuels production is mitigated by low conversion of raw substrates to desired end products. Strategies improve are likely benefit from emerging techniques define molecular systems biology these organisms. Clostridium stercorarium DSM8532T an anaerobic thermophile demonstrated high ethanol on cellulose and hemicellulose. Although several lignocellulolytic enzymes this organism have been well-characterized, details...
Transcriptomes and proteomes of Pseudomonas putida LS46 cultured with biodiesel-derived waste glycerol or free fatty acids, as sole carbon sources, were compared under conditions that either permissive non-permissive for synthesis medium chain length polyhydroxyalkanoates (mcl-PHA). The objectives this study to elucidate mechanisms influence activation biopolymer synthesis, intra-cellular accumulation, monomer composition, determine if these physiologically specific the sources used growth...
In the evaluation of druggability candidate compounds, it was vital to predict oral bioavailability compounds from apparent permeability (Papp) across Caco-2 cell-culture model intestinal epithelium cultured on commercial transwell plate inserts. The study investigate transport characteristics and FL118 (10, 11-Methylenedioxy-20(S)-camptothecin) derivatives 7-Q6 (7-(4-Ethylphenyl)-10, 11-methylenedioxy-20(S)-camptothecin) 7-Q20 (7-(4-Trifluoromethylphenyl)-10,...
Irinotecan and Topotecan are two Camptothecin derivatives (CPTs) whose resistance is associated with the high expression of breast cancer protein (BCRP) P-glycoprotein (P-gp). To reverse this resistance, novel CPTs, FL77-28 (7-(3-Fluoro-4-methylphenyl)-10,11-methylenedioxy-20(S)-CPT) FL77-29 (7-(4-Fluoro-3-methylphenyl)-10,11-methylenedioxy-20(S)-CPT), were synthesized by our group. In study, anti-tumor activities FL77-28, FL77-29, their parent, FL118 (10,11-methylenedioxy-20(S)-CPT),...
Camptothecin (CPT), a natural alkaloid, possesses potent anticancer activity. However, its application was terminated due to low bioavailability and high toxicity. This work evaluated the potential of deoxycholic acid-CPT conjugate (G2) improve oral absorption CPT. Deoxycholic acid significantly reduced cytotoxicity inhibited uptake G2, in vitro. And G2 showed sodium-dependent uptake. In addition, vivo study rats indicated that 2.06-fold higher than The present suggested using bile as...
Here, we report the draft genome sequence of Clostridium termitidis strain CT1112 (DSM 5398), a mesophilic, cellulolytic bacterium that can utilize variety sugars, as well pure cellulose, sole carbon source; it also synthesizes fermentation end products with potential industrial applications.
We describe the draft genome sequence of Pseudomonas putida strain LS46, a novel isolate that synthesizes medium-chain-length polyhydroxyalkanoates. The P. LS46 consists approximately 5.86 million bp, with G+C content 61.69%. A total 5,316 annotated genes and 5,219 coding sequences (CDS) were identified.
Here, we report the draft genome sequence of Clostridium intestinale strain URNW, which can convert biomass to useful products such as biofuels (hydrogen or ethanol) and other soluble end products.
Objective: To explore the effect of online-to-offline (O2O) education combined with peer management model in patients chronic obstructive pulmonary disease (COPD). Methods: Using convenience sampling, 72 COPD who were hospitalized respiratory medicine department a tertiary-level hospital from March to December 2021 selected as study subjects. Randomized grouping was carried out using random number table method, control group given routine health department, and intervention applied O2O...
Abstract Emerging evidence has discovered that circular RNAs play important regulators of nonsmall cell lung cancer (NSCLC), but the role and potential molecular mechanism hsa_circ_100549 (circ_0004630) involved in NSCLC is poorly defined. In this study, circ_0004630, microRNA‐1208 (miR‐1208), leucine‐rich repeat kinase 2 (LRRK2) expression were detected using real‐time quantitative polymerase chain reaction. Cell proliferation, colony formation, apoptosis, invasion assessed...
Tuberculosis (TB) is now the world's second deadliest infectious killer after COVID-19. Human-macrophages and their secreted exosomes play important roles in combating invading Mtb. However, panoramic analysis of underlying immune mechanism for infected macrophages, package or secretion mechanism, anti-TB effect Mtb treated remain poorly understood. Here we conducted comprehensive analyses macrophages with avirulent virulent (H37Ra & H37Rv) exosomes, collected cells corresponding omics...
SUMMARY TNF-α signaling plays a central role in the pathogenesis of various diseases, particularly autoimmune diseases. Screening library composed FDA approved drugs led to identification Terfenadine and its active metabolite Fexofenadine as inhibitors signaling. Both inhibited TNF/NF-κB vitro vivo , ameliorated disease symptoms models, including transgenic mice, collagen-induced arthritis, inflammatory bowel disease. Subsequent studies identified cytosolic phospholipase A2 (cPLA2) novel...
Abstract Klebsiella pneumoniae ( K. pneumonia ) is an important pathogen that can cause severe hospital-/community-acquired infections. To panoramically analyze pneumoniae’s methylation features, we completed the whole genome sequences of 14 strains covering various serotypes, multilocus-sequence typings (MLSTs), clonal groups (CG), viscosity/virulence and drug-resistances, further characterized their methylomes using PacBio-SMRT bisulfite technologies. We identified 15 motifs (13 6mA two...
Background: Emerging evidence has discovered that circular RNAs (circRNAs) play important regulators of non-small cell lung cancer (NSCLC), but the role and potential molecular mechanism hsa_circ_100549 (circ_0004630) involved in NSCLC is poorly defined.Methods: Circ_0004630, microRNA-1208 (miR-1208), leucine-rich repeat kinase 2 (LRRK2) expression was detected using real-time quantitative polymerase chain reaction (RT-qPCR). Cell proliferation, colony formation, apoptosis, invasion were...
旨在克隆鸡H+/肌醇转运蛋白（H+/myoinositol transporter，HMIT）基因的转录序列，并检测HMIT基因在鸡不同组织中的表达情况，以及外源胰岛素处理对HMIT基因相对表达量的影响。本研究以AA肉鸡为试验动物，采用RT-PCR技术克隆鸡HMIT基因全编码区序列，采用生物信息学技术分析其编码蛋白的生物学特性，利用荧光定量PCR检测HMIT基因在大脑、肝、腹脂、腿肌、心、肾、空肠和回肠等组织中的表达情况，并检测其在肾和大脑中进行胰岛素处理120和240 min后的表达情况。结果表明，以NCBI预测的鸡HMIT（XM_001232939.5）序列为模板设计引物，成功克隆出鸡HMIT基因（1 694 bp，GenBank登录号：MN708211）。克隆的鸡HMIT编码区全长1 380 bp，相比XM_001232939.5预测的编码序列少561...
Aim: The resident bacterial microbiome may shape and protect the health of vertebrate host. An array molecules secreted by contribute to ecological stability itself. Material & methods: ELISA, radioactivity, immunofluorescence cytokines measurements were used observe bioactivity colicin Ia level in oviparous viviparous animal circulation. Results: Colicin Ia, a protein antimicrobial produced Escherichia coli, is not present animals at birth, but increases concentration with establishment...
Abstract Background Constipation has been hypothesized to be associated with the increased risk of wheezing or asthma. However, relation remains a subject debate. We conducted this meta-analysis assess whether constipation influence wheezing/asthma. Methods PubMed, Embase, and Web Science were systematically searched for studies published between 1955 January 2022. Two reviewers independently extracted data assessed quality each study. Results pooled using fixed-effect models random-effects...