A5100322001- Ubiquitin and proteasome pathways
- interferon and immune responses
- Cancer-related Molecular Pathways
- Cancer-related gene regulation
- Immune Cell Function and Interaction
- Genetics and Neurodevelopmental Disorders
- RNA modifications and cancer
- PARP inhibition in cancer therapy
- T-cell and B-cell Immunology
- Galectins and Cancer Biology
- Cancer-related molecular mechanisms research
- Inflammasome and immune disorders
- Pancreatic function and diabetes
- Cancer Mechanisms and Therapy
- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Metabolism, Diabetes, and Cancer
- Cytokine Signaling Pathways and Interactions
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
- Immune cells in cancer
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
Shanghai Cancer Institute
2015-2025
Dalian Medical University
2024-2025
Chongqing Medical University
2024-2025
Second Affiliated Hospital of Chongqing Medical University
2024-2025
State Key Laboratory of Oncogene and Related Genes
2013-2024
Renji Hospital
2013-2024
Shanghai Jiao Tong University
2015-2024
Guangzhou Medical University
2020-2024
Chongqing Emergency Medical Center
2024
Chongqing University
2024
Abstract Background The prognosis for hepatocellular carcinoma (HCC) remains suboptimal, characterized by high recurrence and metastasis rates. Although metalloimmunotherapy has shown potential in combating tumor proliferation, metastasis, current apoptosis-based fails to elicit sufficient immune response HCC. Results A smart responsive bimetallic nanovaccine was constructed induce immunogenic cell death (ICD) through pyroptosis enhance the efficacy of cGAS-STING pathway. composed...
Significance Both overexpression of wild-type fused in sarcoma (FUS) protein and missense mutations can be pathogenic a group related neurodegenerative disorders that includes amyotrophic lateral sclerosis frontotemporal lobar degeneration. It is unclear how FUS cause disease human patients. In this work, we generated novel transgenic mouse models expressing low levels mutant FUS, both which recapitulate aspects the diseases. We found profound difference underlying mechanisms by mutation...
Abstract Hyperactivation of the transcriptional factor E2F1 occurs frequently in human cancers and contributes to malignant progression. activity is regulated by proteolysis mediated ubiquitin–proteasome system. However, deubiquitylase that controls ubiquitylation stability remains undefined. Here we demonstrate POH1 stabilizes protein through binding deubiquitylating E2F1. Conditional knockout Poh1 alleles results reduced expression primary mouse liver cells. The POH1-mediated regulation...
Abstract Breast cancer ranks no. 1 in women worldwide, while 60–70% are estrogen receptor alpha positive. The selective modulators, such as tamoxifen, become the effective drugs for controlling ER breast progression. However, tamoxifen resistance will develop during long-time treatment and Thus, further understanding of signaling becomes necessary improvement therapy. Here, we identify TRIM56 a novel regulatory factor signaling. expression is positively correlated with PR samples related to...
Abstract Human spermatogenic cells have not yet been isolated and notably, their global miRNA profiles remain unknown. Here we effectively human spermatogonia, pachytene spermatocytes round spermatids using STA-PUT velocity sedimentation. RT-PCR, immunocytochemistry meiosis spread assays revealed that the purities of were 90% viability these was over 98%. MiRNA microarrays showed distinct among spermatids. Thirty-two miRNAs significantly up-regulated whereas 78 down-regulated between...
IL-25 is an important immune regulator that can promote Th2 response-dependent immunity, inflammation, and tissue repair in asthma, intestinal infection, autoimmune diseases. In this study, we examined the effects of renal ischemic/reperfusion injury (IRI). Treating IRI mice with significantly improved function reduced injury. Furthermore, treatment increased levels IL-4, IL-5, IL-13 serum kidney promoted induction alternatively activated (M2) macrophages kidney. Notably, also frequency type...
Sertoli cells play critical roles in regulating spermatogenesis and they can be reprogrammed to the of other lineages, highlighting that have significant applications reproductive regenerative medicine. The fate determinations are regulated precisely by epigenetic factors. However, expression, roles, targets microRNA (miRNA) human remain unknown. Here we for first time revealed 174 miRNAs were distinctly expressed between Sertoli-cell-only syndrome (SCOS) patients obstructive azoospermia...
Abstract Oncogenic activation of KRAS and its surrogates is essential for tumour cell proliferation survival, as well the development protumourigenic microenvironments. Here, we show that deubiquitinase USP12 commonly downregulated in Kras G12D -driven mouse lung human non-small cancer owing to AKT-mTOR signalling. Downregulation promotes growth fosters an immunosuppressive microenvironment with increased macrophage recruitment, hypervascularization, reduced T activation. Mechanistically,...
Abstract Protein acetylation has a crucial role in energy metabolism. Here we performed the first large-scale profiling of acetylome rat islets, showing that almost all enzymes core metabolic pathways related to insulin secretion were acetylated. Label-free quantitative islets response high glucose revealed hyperacetylation involved fatty acid β-oxidation (FAO), including trifunctional enzyme subunit alpha (ECHA). Acetylation decreased protein stability ECHA and its ability promote FAO. The...
Rationale: Sirtuins are NAD+-dependent protein deacylases known to have protective effects against age-related diseases such as diabetes, cancer, and neurodegenerative disease. SIRT2 is the only primarily cytoplasmic isoform its overall role in glucose homeostasis remains uncertain. Methods: SIRT2-knockout (KO) rats were constructed evaluate of homeostasis. The effect on β-cell function was detected by investigating morphology, insulin secretion, metabolomic state islets. deacetylation...
Natural killer (NK) cells are known as innate immune lymphocytes that respond rapidly when challenged by pathogens but little is about adaptive features including memory related to NK from human beings. In the present study, we demonstrate for first time expressing memory-associated marker CD45RO were persistent in pleural fluid (PFCs) tuberculous patients. CD45RO+ produced significantly more interferon-γ and cytotoxic compared with CD45RO− PFCs stimulated interleukin-12 (IL-12)....
Quantitative reverse transcription PCR (qRT-PCR) is becoming increasingly important in the effort to gain insight into molecular mechanisms underlying adipogenesis. However, expression profile of a target gene may be misinterpreted due unstable reference genes under different experimental conditions. Therefore, this study, we investigated stability 10 commonly used during 3T3-L1 adipocyte differentiation. The mRNA levels glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and transferrin...
Abstract Inflammasome activation is essential for host defence against invading pathogens, but also involved in various forms of inflammatory diseases. The processes that control inflammasome activity are thus important averting excessive immune responses and tissue damage. Here we show the deubiquitinase POH1 negatively regulates response triggered by activation. deficiency macrophages enhances mature IL-1β production without significant alterations priming ASC-caspase-1 In WT macrophages,...
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Background: Most anticancer agents induce tumor apoptosis, but they often lack immunogenicity and display limited success when combined with mainstream immunotherapies, thus killing cancer cells through multiple cell death modalities as well switching immune-off tumors to immune-on is a strategy great promise. To this end, we developed CPApoptosis (cuproptosis, pyroptosis, apoptosis) nano-actuator for immunologically cold solid tumors. Methods: In study, elesclomol (ES), mitochondrial...
BackgroundHyper-activation of TGF-β signaling is critically involved in progression hepatocellular carcinoma (HCC). However, the events that contribute to dysregulation pathway HCC, especially at post-translational level, are not well understood.MethodsAssociations deubiquitinase POH1 with activity and outcomes HCC patients were examined by data mining online datasets, immunohistochemistry analyses using human specimens, spearman correlation survival analyses. The effects on ubiquitination...
Abstract Neuroendocrine carcinoma (NEC) of the gallbladder (GB-NEC) is a rare but extremely malignant subtype cancer (GBC). The genetic and molecular signatures GB-NEC are poorly understood; thus, targeting currently unavailable. In present study, we applied whole-exome sequencing (WES) technology to detect gene mutations predicted somatic single-nucleotide variants (SNVs) in 15 cases 22 general GBC. GB-NECs, C > T mutation was predominant among 6 types SNVs. TP53 showed highest frequency...