A5081761423- RNA modifications and cancer
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Polymer composites and self-healing
- Virus-based gene therapy research
- Advancements in Battery Materials
- Graphene research and applications
- Supercapacitor Materials and Fabrication
- Polymer Nanocomposites and Properties
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Fiber-reinforced polymer composites
- Nerve injury and regeneration
- Concrete and Cement Materials Research
- Epoxy Resin Curing Processes
- Advanced Photocatalysis Techniques
- Retinal Development and Disorders
- RNA and protein synthesis mechanisms
- Injection Molding Process and Properties
- Mesoporous Materials and Catalysis
- Electrospun Nanofibers in Biomedical Applications
- Transition Metal Oxide Nanomaterials
- Silk-based biomaterials and applications
- Iron oxide chemistry and applications
Jilin University
2018-2025
Jilin Medical University
2023-2025
Chinese Academy of Sciences
2024
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2024
Yixing People's Hospital
2017-2018
Jiangsu University
2017-2018
Soochow University
2009-2017
Second Affiliated Hospital of Soochow University
2009-2017
University of Minnesota
2010-2014
H.B. Fuller (United States)
2014
Abstract Numerous benefits of porous electrode materials for lithium ion batteries (LIBs) have been demonstrated, including examples higher rate capabilities, better cycle lives, and sometimes greater gravimetric capacities at a given compared to nonporous bulk materials. These properties promise advantages LIBs in electric hybrid vehicles, portable electronic devices, stationary electrical energy storage. This review highlights methods synthesizing by templating template‐free discusses how...
The toughening effects of graphene and graphene‐derived materials on thermosetting epoxies are investigated. Graphene with various structures surface functional groups incorporated into an epoxy resin by in situ polymerization. oxide (GO) GO modified amine‐terminated poly(butadiene‐acrylonitrile) (ATBN) chosen to improve the dispersion nanosheets increase their interfacial adhesion. An impressive effect is observed less than 0.1 wt% graphene. A maximum toughness at loadings as small 0.02 or...
Natural vermiculite was modified by cation exchange with long-chain quaternary alkylammonium salts and then dispersed in polyether-based polyols different structures ethylene oxide/propylene oxide ratios. The dispersions were evaluated X-ray scattering rheology. In all polyol tested, intercalated into the interlayers. Also, significant shear thinning behavior observed. A large interlayer spacing of ∼90 Å achieved one suitable for polyurethane elastomer synthesis. made this polyol, clay...
A facile route for the preparation of WO3·0.33H2O hexagonal-shaped nanodiscs composed nanosheets via hydrothermal treatment an aqueous peroxo-polytungstic acid solution has been demonstrated first time. The obtained have average diameter 200 nm and a mean thickness several tens nanometers. effects conditions such as temperature precursor concentration on crystalline phase morphology products studied systematically. with orthorhombic structure regular hexagonal can be synthesized in wide...
Currently, the Cas9 and Cas12a systems are widely used for genome editing, but their ability to precisely generate large chromosome fragment deletions is limited. Type I-E CRISPR mediates broad unidirectional DNA degradation, controlling size of Cas3-mediated has proven elusive thus far. Here, we demonstrate that endonuclease deactivation (dCas9) can control large-fragment in mammalian cells. In addition, report elimination Y precise retention Sry gene mice using CRISPR/Cas3 dCas9-controlled...
Free-standing, flexible V2O5-graphene composite films were prepared by simple filtration of aqueous dispersions V2O5 nanowires and graphene sheets. preferentially oriented along the plane film as they sandwiched between stacked sheets in film. The content composites was adjusted simply varying relative amount dispersions. Thermal annealing at 300°C increased conductivity films. Due to their integrated structure high flexibility, directly usable electrodes. In binder-free, sandwich-type...
Tay-Sachs disease (TSD) is a progressive neurodegenerative disorder due to an autosomal recessively inherited deficiency of β-hexosaminidase A (HexA) 1 .The four-bases (TATC) insertion in exon 11 the HEXA (HEXA ins TATC) accounts for 80% from Ashkenazi Jewish population 2 .However, no typical clinical phenotypes, such as neurological abnormalities, restricted pattern distribution GM2-ganglioside and membranous cytoplasmic bodies brain, were observed -/-mouse models, difference ganglioside...
Transplantation of olfactory ensheathing cells (OECs) is a potential treatment for spinal cord injury (SCI). However, this process lacks extracellular matrix guiding cell growth, tissue morphogenesis, and remodeling. In order to solve problem, we fabricated silk fibroin scaffolds (SFS) with different fiber diameters by electrospinning. The behaviors OECs on 300 1800 nm SFS were studied analyzing morphological feature, distribution, proliferation. results showed the good guide growth....
5-Methylcytosine (m 5 C) is one of the major post-transcriptional modifications in mRNA and highly involved pathogenesis various diseases. However, capacity existing assays for accurately comprehensively transcriptome-wide m C mapping still needs improvement. Here, we develop a detection method named DRAM (deaminase reader protein assisted RNA methylation analysis), which deaminases (APOBEC1 TadA-8e) are fused with proteins (ALYREF YBX1) to identify sites through deamination events...
5-Methylcytosine (m 5 C) is one of the post-transcriptional modifications in mRNA and involved pathogenesis various diseases. However, capacity existing assays for accurately comprehensively transcriptome-wide m C mapping still needs improvement. Here, we develop a detection method named DRAM (deaminase reader protein assisted RNA methylation analysis), which deaminases (APOBEC1 TadA-8e) are fused with proteins (ALYREF YBX1) to identify sites through deamination events neighboring sites....
5-Methylcytosine (m 5 C) is one of the posttranscriptional modifications in mRNA and involved pathogenesis various diseases. However, capacity existing assays for accurately comprehensively transcriptome-wide m C mapping still needs improvement. Here, we develop a detection method named DRAM (deaminase reader protein assisted RNA methylation analysis), which deaminases (APOBEC1 TadA-8e) are fused with proteins (ALYREF YBX1) to identify sites through deamination events neighboring sites. This...
The role of nanoparticles in the fracture toughness glass fiber‐reinforced polyester composites (GFRPs) was investigated. An unsaturated resin (UPR) and a vinyl ester (VER) were toughened by two distinct methods: (1) incorporating very low loading (0.04 wt%) alkyl or modified graphene oxide (mGO); (2) adding core‐shell rubber (CSR, 1 5 wt%). For unreinforced resins, results from compact tension tests revealed that 0.04 wt% mGO increased ( G IC_Resin ) UPR VER as much 49 35%, respectively,...
The largest use of polyurethane (PU) is as closed cell rigid foams for thermal insulation. One problem loss blowing gases, which leads to slow increase in conductivity. PU composites with plate-like nanofillers create a diffusion barrier, reducing gas transport and slowing insulation aging. In this research, new situ intercalative polymerization described disperse vermiculite (VMT) PU. When VMT was modified by cation exchange long-chain quaternary ammonium, the dispersion methylene diphenyl...
Retinoids (retinol and its derivatives) are required for the development maintenance of normal physiological functions ovary. However, mechanisms underlying regulation ovarian retinoid homeostasis during follicular remain unclear. The present study determined levels expression genes involved in retinol uptake metabolic pathway ovaries follicle-stimulating hormone (FSH)-treated mice granulosa cells treated with FSH using ultra performance liquid chromatography (UPLC) combined quadrupole...
Oxide nanoparticles (NPs) are typically synthesized from the assembly of atoms, ions or other species by bottom-up methods. Here we report an alternative, top-down approach as a general route to synthesize porous and nonporous oxide/phosphate nanocubes. The method is based on construction three-dimensionally ordered macroporous (3DOM) structures via colloidal crystal templating, followed spontaneous disassembly these into particulate building blocks assisted introduction amphiphilic...
Abstract Background Nme2ABE8e has been constructed and characterized as a compact, accurate adenine base editor with less restrictive dinucleotide protospacer-adjacent motif (PAM: N4CC) but low editing efficiency at challenging loci in human cells. Here, we engineered subset of domain-inlaid Nme2Cas9 editors to bring the deaminase domain closer nontarget strand improve efficiency. Results Our results demonstrated that Nme2ABE8e-797 inserted between amino acids 797 798 significantly increased...
Adenine base editors (ABEs) are promising therapeutic gene editing tools that can efficiently convert targeted A•T to G•C pairs in the genome. However, large size of commonly used ABEs based on SpCas9 hinders its delivery vivo using certain vectors such as adeno-associated virus (AAV) during preclinical applications. Despite a number approaches having previously been attempted overcome challenge, including split Cas9-derived and numerous domain-deleted versions editors, whether editor (BE)...