Mmigration of undifferentiated neural progenitors is critical for the development and repair nervous system. However, mechanisms factors that regulate migration are not well understood. Here, we show vascular endothelial growth factor (VEGF)-A, a major angiogenic factor, guides directed do display antigenic markers neuron- or glia-restricted precursor cells. We demonstrate progenitor cells express both VEGF receptor (VEGFR) 1 VEGFR2, but signaling through VEGFR2 specifically mediates...

Abstract Although transplantation of human embryonic stem cells (hESCs)‐derived neural precursors (NPs) has been demonstrated with some success for nervous repair in small animal model, control the survival, and directional differentiation these is still challenging. Meanwhile, notion that using suitable scaffolding materials to growth grafted hESC‐derived NPs raises hope better clinical repair. In this study, we cultured on Tussah silk fibroin (TSF)‐scaffold different diameter (i.e., 400...

Nerve tissue engineering has been one of the promising strategies for regenerative treatment in patients suffering from neural loss, but considerable challenges remain before it is able to progress toward clinical application. It demonstrated that transplantation cells combination with physically or chemically modified biomaterials provides better environments neurite outgrowth and further promotes axonal regeneration animal models spinal cord injury. In this study, neurons astrocytes were...

Abstract Wnt/β-catenin signaling plays a crucial role in the migration of mesenchymal stem cells (MSCs). However, our study has revealed an intriguing phenomenon where Dickkopf-1 (DKK1), inhibitor signaling, promotes MSC at certain concentrations ranging from 25 to 100 ng/mL while inhibiting Wnt3a-induced higher concentration (400 ng/mL). Interestingly, DKK1 consistently inhibited phosphorylation LRP6 all concentrations. We further identified cytoskeleton-associated protein 4 (CKAP4),...

Alcohol drinking can cause hyperuricemia (HUA), but few studies have explored alcohol abstinence's health effects, particularly the abstinence duration on HUA. This study aimed to evaluate associations of with HUA in rural Chinese adults. was a cross-sectional 38,855 participants (15,371 males and 23,484 females) using baseline data (2015–2017) from Henan Rural Cohort Study. A questionnaire survey collected information consumption patterns. Multivariate logistic regression analyses were used...

Exploring effective, prompt and universally applicable approaches for inducing the differentiation of glioblastoma (GBM) into terminally differentiated cells, such as astrocytes or neurons that cease cell division, is pivotal success GBM therapy. In this study, a neuronal-specific promoter-reporter system was employed to screen small molecules promote neural differentiation. The cocktail YFSS, consisting Y27632, Forskolin, SB431542 SP600125, which selectively targets ROCK, cAMP, TGF-β JNK...

Transplantation of olfactory ensheathing cells (OECs) is a potential treatment for spinal cord injury (SCI). However, this process lacks extracellular matrix guiding cell growth, tissue morphogenesis, and remodeling. In order to solve problem, we fabricated silk fibroin scaffolds (SFS) with different fiber diameters by electrospinning. The behaviors OECs on 300 1800 nm SFS were studied analyzing morphological feature, distribution, proliferation. results showed the good guide growth....

Mesenchymal stem cells (MSCs) have the potential to treat various tissue damages, but very limited number of that migrate damaged region strongly restricts their therapeutic applications. Full understanding mechanisms regulating MSC migration will help improve ability and effects. Increasing evidence shows microRNAs play important roles in regulation migration. In present study, we reported miR-9-5p was upregulated hepatocyte growth factor -treated MSCs with high ability. Overexpression...

The chemotactic migration of mesenchymal stem cells (MSCs) is fundamental for their use in cell-based therapies, but little known about the molecular mechanisms that regulate directed migration. MicroRNAs (miRNAs) participate regulation a large variety cellular processes. However, roles regulating responses MSCs to hepatocyte growth factor (HGF) remain elusive. Here, we found microRNA-221 (miR-221) and microRNA-26b (miR-26b) were upregulated subjected HGF. Overexpression miR-221 or miR-26b...

Three different single crystal forms of an azaacene derivative with fluorescence quantum yields have been obtained and the relationship between their structures studied.

Mesenchymal stem cells (MSCs) hold great promise in neural regeneration, due to their intrinsic neuronal potential and migratory tropism damaged nervous tissues. However, the chemotactic signals mediating migration of MSCs remain poorly understood. Here, we investigated regulatory roles for focal adhesion kinase (FAK) Rac1 vascular endothelial growth factor (VEGF)‐stimulated differentiation. We found that various differentiation states show significant different responses VEGF 24‐h...

Adenosine triphosphate (ATP)-binding cassette transporter A1 (ABCA1) influences hepatic cholesterol transportation. Accumulation of leads to fatty liver disease, which is improved by glucagon-like peptide 1 (GLP-1) in diabetes. Therefore, we analyzed the molecular mechanism regulation ABCA1 GLP-1 analogue exendin-4. Hepatic expression and transcription were checked western blotting, real-time polymerase chain reaction (PCR), luciferase assay HepG2 cells. Chromatin immunoprecipitation (ChIP)...

Silk fibroin of the silkworm Bombyx mori has been studied extensively, while research on Antheraea pernyi silk (A. SF) in biomaterials is only at an early stage. In this study, attachment, morphology, growth and phenotype human bone marrow derived mesenchymal stem cells (hBMSCs) cultured regenerated A. SF films were vitro. The results indicated that attachment hBMSCs was almost same as collagen films. MTT cell counting analyses demonstrated better than controls. Moreover, electron scanning...

Recently, mesenchymal stem cells (MSCs) have been extensively used for cell-based therapies in neuronal degenerative disease. Although much effort has devoted to the delineation of factors involved migration MSCs, relationship between chemotactic responses and differentiation status these remains elusive. Here, we report that MSCs varying neural states display different hepatocyte growth factor (HGF): first, number chemotaxing optimal concentrations HGF induced peak varied greatly; second,...

Neural scaffolds consisting of dermal fibroblast-reprogrammed neurons and 3D silk fibrous materials promote repair spinal cord injury.

In this study, we investigated how human umbilical cord mesenchymal stem cells exerted a neuroprotective effect via antiapoptotic mechanisms in neonatal hypoxic-ischemic encephalopathy rat model. A total of 78 10-day old (P10) rats were used. After collected from cords and amplified culture, they administered to subjects 1 h after induced treatment. The short-term (48 h) long-term (28 day) outcomes evaluated treatment using neurobehavioral function assessment. Triphenyltetrazolium chloride...

In this study, Schwann cells, at a density of 1 × 10(5) cells/well, were cultured on regenerated silk fibroin nanofibers (305 ± 84 nm) prepared using the electrospinning method. cells appeared more ordered, their processes extended further, and they formed extensive complex interconnections. addition, had no impact proliferation or secretion ciliary neurotrophic factor, brain-derived factor nerve growth factor. These findings indicate that electrospun can promote cell adhesion,...

As an essential trace element in the body, selenium is associated with development of many diseases. The purpose this study was to explore association between dietary intake and new-onset stroke risk Chinese adults.Adults aged ≥18 years China Health Nutrition Survey (CHNS) from 2004 2015 were enrolled. Participants divided into five groups according quintile intake: Q1 (≤ 29.80 μg/day), Q2 (29.80-38.53 Q3 (38.53-47.23 Q4 (47.23-60.38 Q 5(>60.38 μg/day). Cox proportional-hazards model used...