A5037589519- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Congenital Heart Disease Studies
- Protein Kinase Regulation and GTPase Signaling
- Chemical Synthesis and Analysis
- Advanced Radiotherapy Techniques
- Medical Imaging Techniques and Applications
- Cardiovascular Issues in Pregnancy
- Lung Cancer Diagnosis and Treatment
- Radiation Therapy and Dosimetry
- Prostate Cancer Treatment and Research
- Effects of Radiation Exposure
- Neurobiology and Insect Physiology Research
- Bipolar Disorder and Treatment
- Adenosine and Purinergic Signaling
- Regulation of Appetite and Obesity
- Ion channel regulation and function
- Cardiovascular Conditions and Treatments
- Cancer, Stress, Anesthesia, and Immune Response
- Cardiac Structural Anomalies and Repair
- DNA Repair Mechanisms
- Apelin-related biomedical research
- GABA and Rice Research
- Blood disorders and treatments
- Skin and Cellular Biology Research
Johns Hopkins University
2021-2024
Heart Kids
2024
Peter MacCallum Cancer Centre
2018-2023
National Heart Foundation of Australia
2016-2021
UNSW Sydney
2021
Queen's Medical Centre
1997-1999
University of Nottingham
1998
Garvan Institute of Medical Research
1989-1995
St Vincent's Hospital Sydney
1989-1995
St Vincent's Hospital
1992-1994
Neuropeptide Y (NPY) is one of the most abundant neuropeptides in mammalian nervous system and exhibits a diverse range important physiological activities, including effects on psychomotor activity, food intake, regulation central endocrine secretion, potent vasoactive cardiovascular system. Two major subtypes NPY receptor (Y1 Y2) have been defined by pharmacological criteria. We report here molecular cloning cDNA sequence encoding human corrected for rat homologue. Analysis this confirms...
The protein kinase C (PKC) family of serine-threonine kinases comprises at least eight members. These are differentially expressed, show varying affinities for activators such as Ca2+ and lipid species, therefore thought to play wide-ranging roles in the regulation cellular processes differentiation, growth, secretion. aim this study was identify new PKC isoforms insulin-secreting cell line RINm5F that might be activated by alterations metabolism accompany nutrient-stimulated insulin...
Dopamine, a major neurotransmitter in the mammalian nervous system, exerts its physiological effects through receptors of G-protein-coupled receptor superfamily. Two classes dopamine receptor, D1 and D2, are distinguishable by both biochemical pharmacological criteria. activate adenylyl cyclase, whereas D2 class inhibits this second messenger system. subtypes human generated alternate splicing RNA transcript single gene. These two forms, termed D2A (long) D2B (short), differ insertion 29...
A cDNA clone encoding the human homolog of bovine LCR1 was isolated from a lung library. The 1,670-bp-long nucleotide sequence predicts single open reading frame 352 amino acids, with 92% acid identity to reported represent neuropeptide Y (NPY) Y3 receptor. shares features common many other G-protein-coupled receptors, including seven transmembrane regions and putative glycosylation phosphorylation sites. Polymerase chain reaction (PCR) analysis human–hamster hybrid cell DNA reveals that...
The structural diversity of the human D2 dopamine receptor was examined at nucleic acid level. Sequence analysis cDNA clones isolated from brain and pituitary libraries polymerase chain reaction (PCR) rat RNA genomic DNA demonstrate presence a predominant subtype, D2A. D2A subtype differs D2B previously described in RNA, that an additional 29 amino acids are present putative third cytoplasmic domain, region thought to be important for coupling different G-proteins. demonstration intron...
Neuropeptide tyrosine (NPY) is the predominant peptide in innervation of many human tissues and considered to play a role regulation blood flow, gastrointestinal secretion motility, renal function. Three NPY receptors have been identified (Y1, Y2, Y3) cDNAs encoding Y1 bovine Y3 recently cloned. We demonstrated expression receptor subtype several fetal adult tissues, including colon, kidney, adrenal gland, heart, placenta. A single transcript was (approximately 2.2 kb) localized tissue...
These first Australian National Standards of Care for Childhood-onset Heart Disease (CoHD Standards) have been developed to inform the healthcare requirements CoHD services and enable all patients, families carers impacted by (paediatric adult congenital heart disease [ACHD]) live their best healthiest lives. The are designed provide clarity certainty required deliver excellent, comprehensive, inclusive, equitable care across Australia carers, offer an iterative roadmap future these...
A 14-kilobase pair (kb) region of genomic DNA encoding the human neuropeptide Y Y1-receptor gene including 3'- and 5'-flanking sequences has been cloned localized to chromosome 4q(31.3-32). In contrast contiguous structure most G protein-coupled receptor genes, NPY Y1 is divided into three exons. small 5'-exon mRNA untranslated separated by a 6-kb intron from second exon. The coding interrupted intron, containing an in-frame stop codon, shortly after proposed fifth transmembrane domain....
Neuropeptide Y (NPY) and norepinephrine, found co-localized in sympathetic neurons innervating blood vessels, exert synergistic responses on vasoconstriction. To examine the signaling mechanisms involved, free of complications associated with mixed receptor populations, we have established a stable Chinese hamster ovary cell line expressing both Y1-NPY α1b-adrenergic receptors. Occupation either species, 100 nM peptide YY (PYY) or 10 μM phenylephrine (PE), respectively, resulted rapid...
In prostate cancer patients, imaging of bone metastases is enhanced through the use sodium fluoride positron emission tomography (18F-NaF PET/CT). This technique shows areas osteoblastic activity and blood flow. this work, 18F-NaF PET/CT was investigated for response assessment to single fraction stereotactic ablative body radiotherapy (SABR) in patients.Patients with a prospective trial treated SABR received scan prior 6 months post-SABR. The SUVmax tumour determined difference between...
Four-Dimensional Gallium 68 Ventilation-Perfusion Positron Emission Tomography (68Ga-4D-V/Q PET/CT) allows for dynamic imaging of lung function. To date there has been no assessment the feasibility adapting radiation therapy plans to changes in function imaged at mid-treatment using 68Ga-4D-V/Q PET/CT. This study assessed potential reductions dose functional when were adapted avoid timepoint volumetric arc radiotherapy (VMAT).A prospective clinical trial (U1111-1138-4421) was performed...