A5015214221- Influenza Virus Research Studies
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- interferon and immune responses
- Acute Myocardial Infarction Research
- Virus-based gene therapy research
- Viral Infections and Vectors
- RNA and protein synthesis mechanisms
- Animal Disease Management and Epidemiology
- Immune Response and Inflammation
- COVID-19 Clinical Research Studies
- Virology and Viral Diseases
- Biosensors and Analytical Detection
- Blood Pressure and Hypertension Studies
- CAR-T cell therapy research
- Sex and Gender in Healthcare
- HIV Research and Treatment
- Clinical practice guidelines implementation
- Healthcare Systems and Public Health
- Frailty in Older Adults
- Advanced Biosensing Techniques and Applications
- Antiplatelet Therapy and Cardiovascular Diseases
Guangzhou Medical University
2015-2025
State Key Laboratory of Respiratory Disease
2015-2025
First Affiliated Hospital of Guangzhou Medical University
2014-2025
Macau University of Science and Technology
2024
Kingmed Diagnostics
2024
Instituto Maimónides de Investigación Biomédica de Córdoba
2024
Communication University of China
2021
Capital Medical University
2007-2019
Beijing Anzhen Hospital
2007-2019
Guangzhou Eighth People's Hospital
2018
Abstract The rapid spread of coronavirus SARS-CoV-2 greatly threatens global public health but no prophylactic vaccine is available. Here, we report the generation a replication-incompetent recombinant serotype 5 adenovirus, Ad5-S-nb2, carrying codon-optimized gene encoding Spike protein (S). In mice and rhesus macaques, intramuscular injection with Ad5-S-nb2 elicits systemic S-specific antibody cell-mediated immune (CMI) responses. Intranasal inoculation both pulmonary responses weaker CMI...
Preventing and treating influenza virus infection remain a challenge because of incomplete understanding the host–pathogen interactions, limited therapeutics lack universal vaccine. So far, methods for monitoring course with in real time living animals are lacking. Here we report visualization viral mice using an engineered replication-competent A carrying luciferase reporter gene. After intranasal inoculation, bioluminescence can be detected chest nasopharyngeal passage mice. The intensity...
Influenza A virus poses serious health threat to humans. Neutralizing antibodies against the highly conserved M2 ion channel is thought offer broad protection influenza viruses. Here, we screened synthetic Camel single-domain antibody (VHH) libraries native protein. One of isolated VHHs, M2-7A, specifically bound M2-expressed cell membrane as well virion, inhibited replication both amantadine-sensitive and resistant viruses in vitro, protected mice from a lethal challenge. Moreover, M2-7A...
Influenza A virus infection can arrest autophagy, as evidenced by autophagosome accumulation in infected cells. Here, we report that this be inhibited amantadine, an antiviral proton channel inhibitor, amantadine-sensitive cells or expressing influenza matrix protein 2 (M2). Thus, M2 activity plays a role blocking the fusion of autophagosomes with lysosomes, which might key mechanism for arresting autophagy.
Current design of Zika virus (ZIKV) vaccine mainly considered envelope (E) as the major target antigen. Non-structural protein NS1 was seldom considered. Herein, we generated three adenovirus-vectored vaccines carrying E (Ad2-E), or premembrane/membrane (prM/M) with (Ad2-prME), in addition to prM/M (Ad2-prME-NS1). Ad2-prME induced higher neutralizing antibody response ZIKV than Ad2-E, suggesting is important for folding immunogenic E. Most intriguingly, Ad2-prME-NS1 elicited best viral...
Recent outbreaks of avian influenza and ongoing virus reassortment have drawn focus on spill-over infections. The increase in human infections with highly pathogenic H5N6 its high fatality rate posed a potential threat, necessitating the search for more effective treatment.
The COVID-19 pandemic caused by SARS-CoV-2 has had a persistent and significant impact on global public health for 4 years. Recently, there been resurgence of seasonal influenza transmission worldwide. co-circulation viruses results in dual burden communities. Additionally, the potential zoonotic viruses, such as avian Influenza A/H5N1 A/H7N9, remains concern. Therefore, combined vaccine against all these respiratory diseases is urgent need. mRNA vaccines, with their superior efficacy, speed...
Background Many studies have examined gender related differences in the presenting symptoms, management and prognosis of patients with acute coronary syndrome (ACS). Much data are available from industrialized countries, which ACS is a major cause morbidity mortality, but relatively little information has been obtained China, where an epidemic cardiovascular disease starting to emerge. The purpose this study was assess clinical practice national Chinese sample. Methods A total 12 medical...
The Zika virus (ZIKV) outbreak and its link to microcephaly triggered a public health concern. To examine antibody response in patient infected with ZIKV, we used single-cell PCR clone 31 heavy light chain-paired monoclonal antibodies (mAbs) that bind ZIKV envelope (E) proteins isolated from memory B cells of ZIKV-infected patient. Three mAbs (7B3, 1C11, 6A6) showed the most potent broad neutralization activities against African, Asian, American strains were selected for further analysis....
ABSTRACT Replication-competent influenza viruses carrying reporter genes are of great use for basic research, screening antiviral drugs, and neutralizing antibodies. In this study, two recombinant A with a neuraminidase (NA) segment harboring enhanced green fluorescent protein (EGFP) in the background A/PR/8/34 (PR8) were generated. The viral RNA (vRNA)-specific packaging signals NA largely retained efficient packaging. An “autocleave” 2A peptide sequence, which was inserted at N terminus or...
The COVID-19 pandemic caused by SARS-CoV-2 viruses has had a persistent and significant impact on global public health for four years. Recently, there been resurgence of seasonal influenza transmission worldwide. co-circulation results in dual burden communities. Additionally, the potential zoonotic viruses, such as avian Influenza A/H5N1 A/H7N9, remains concern. Therefore, combined vaccine against all these respiratory diseases is urgent need. mRNA vaccines, with their superior efficacy,...
In 2022, we assessed avian influenza A virus subtype H5N6 seroprevalence among the general population in Guangdong Province, China, amid rising numbers of human infections. Among tested samples, found 1 to be seropositive, suggesting that poses a low but present risk population.
In the post-COVID-19 era, co-circulation of respiratory viruses, including influenza, SARS-CoV-2, and syncytial virus (RSV), continues to have significant health impacts presents ongoing public challenges. Vaccination remains most effective measure for preventing viral infections. To address concurrent circulation these extensive efforts been dedicated development combined vaccines. These vaccines utilize a range platforms, mRNA-based vaccines, vector subunit providing opportunities in...
ABSTRACT Adenovirus has been extensively exploited as a vector platform for delivering vaccines. However, preexisting antiadenovirus immunity is the major stumbling block application of adenovirus-vectored In this study, we found that freshly isolated peripheral blood mononuclear cells (PBMCs), mostly CD14 + cells, from adenovirus serotype 5 (Ad5)-seropositive primates (humans and rhesus macaques) can be efficiently infected with Ad5 in vitro . On basis observation, novel strategy based on...
Background The outbreaks of emerging infectious diseases caused by pathogens such as SARS coronavirus, H5N1, H1N1, and recently H7N9 influenza viruses, have been associated with significant mortality morbidity in humans. Neutralizing antibodies from individuals who recovered an infection confer therapeutic protection to others infected the same pathogen. However, survivors may not always be available for providing plasma or cloning monoclonal (mAbs). Methodology/Principal Findings genome...
H5N1 influenza candidate vaccine viruses were developed using the "6+2" approach. The hemagglutinin (HA) and neuraminidase (NA) genes derived from popular virus remaining six internal segments A/Puerto Rico/8/34 strain (H1N1, PR8). However, some of these strains have been reported to produce relatively low yields in manufacture. In this study, we found that NA vRNA A/Vietnam/1194/2004 (H5N1, VN1194) was poorly packaged into recombinant with a backbone PR8 genes, which resulted formation...
Since the first outbreak in 2013, influenza A (H7N9) virus has continued emerging and caused over five epidemic waves. Suspected antigenic changes of H7N9 based on hemagglutination inhibition (HI) assay during fifth have prompted update candidate vaccine viruses (CVVs). In this study, we comprehensively compared serological cross-reactivities induced by hemagglutinins (HAs) earlier CVV A/Anhui/1/2013 (H7/AH13) updated A/Guangdong/17SF003/2016 (H7/GD16). We found that although H7/GD16 showed...