A5009590614- SARS-CoV-2 and COVID-19 Research
- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Pneumocystis jirovecii pneumonia detection and treatment
- COVID-19 Clinical Research Studies
- Polyomavirus and related diseases
- Fungal Infections and Studies
- Tryptophan and brain disorders
- Antifungal resistance and susceptibility
- Fish Ecology and Management Studies
- Full-Duplex Wireless Communications
- vaccines and immunoinformatics approaches
- Pneumonia and Respiratory Infections
- Animal Virus Infections Studies
- Parvovirus B19 Infection Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- HIV Research and Treatment
- Aquatic Invertebrate Ecology and Behavior
- Diatoms and Algae Research
- Immunotherapy and Immune Responses
- Antenna Design and Analysis
- HIV/AIDS drug development and treatment
- Freshwater macroinvertebrate diversity and ecology
- Microplastics and Plastic Pollution
- Salivary Gland Disorders and Functions
Zhejiang University
2018-2025
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2018-2025
Chinese Academy of Fishery Sciences
2022-2024
First Affiliated Hospital Zhejiang University
2018-2024
Ministry of Agriculture and Rural Affairs
2022-2024
Key Laboratory of Guangdong Province
2022-2024
National Clinical Research Center for Digestive Diseases
2022-2024
Abstract Background Although gut microbiota dysbiosis has been reported in HIV infected individuals recently, the relationship between and immune activation patients with different responses to highly active antiretroviral therapy (HAART) is still not well understood. Gut were studied 36 non-HIV-infected subjects (healthy controls) 58 HIV-infected individuals, including 28 immunological responders (IR) 30 non-responders (INR) (≥500 < 200 CD4+ T-cell counts/μl after 2 years of HIV-1 viral...
Purpose This study was conducted in order to properly understand whether prior seasonal human coronavirus (HCoV) immunity could impact the potential cross-reactivity of humoral responses induced by SARS-CoV-2 vaccine, thereby devising universal vaccines for future outbreaks. Methods We performed enzyme-linked immunosorbent assay (ELISA) quantify immunoglobulin G (IgG) antibody levels spike (S) protein and S1 subunit HCoVs (HCoV-OC43, HCoV-HKU1, HCoV-NL63, HCoV-229E), ELISA [anti-RBD...
Introduction The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has been posing a threat to global public health. Although broadly neutralizing antibodies have used prevent or treat corona virus disease 2019 (COVID-19), new emerging variants proven resistant these antibodies. Methods In this study, we isolated receptor binding domain (RBD)-specific memory B cells using single-cell sorting method from two COVID-19 convalescents and expressed the antibody test...
The role of the oral microbiota in HIV-infected individuals deserves attention as either HIV infection or antiretroviral therapy (ART) may have effect on diversity and composition microbiome. However, few studies addressed its interplay with different immune responses to ART individuals. Salivary activation were studied 30 immunological responders (IR) 34 non-responders (INR) (≥500 < 200 CD4 + T-cell counts/μl after 2 years HIV-1 viral suppression, respectively) no comorbidities. Metagenome...
Abstract Background Patients infected with HIV are at high risk of developing Epstein-Barr Virus (EBV)-related diseases. The genotype and viral biological behavior EBV infection in patients human immunodeficiency virus-1 (HIV) China remain unclear. This study analyzed the characteristics southeastern China. Methods A total 162 HIV-infected 52 without were enrolled this study. load blood was determined by fluorescence quantitative PCR. typing performed using saliva according to polymorphisms...
BK polyomavirus (BKPyV) is an opportunistic infectious pathogen that associated with hemorrhagic cystitis and nephropathy, mainly in transplant recipients human immunodeficiency virus 1 (HIV-1) infected patients. However, molecular characterization studies of BKPyV China are rare. This study was designed to elucidate the prevalence determine main subtypes among HIV-1-infected patients southeastern China. In addition, increased incidences for reactivation were analyzed. The isolated DNA...
Experimental transmission of the spontaneous rabbit-pox discussed in preceding communication was attempted early course pandemic. Since result bacteriological examinations together with clinical picture condition made it appear unlikely that we were dealing any usual bacterial agents, initial experiments carried out Berkefeld “V” filtrates various organs and tissues from rabbits showing well marked symptoms disease. In other experiments, unfiltered suspensions used. The employed included:...
Abstract Background The effects of cryptococcemia on patient outcomes in those with or without HIV remain unclear. Methods One hundred and seventy‐nine patients were enrolled this retrospective study. Demographic characteristics, blood test results outcome compared between the two groups. Results diagnosis time Cryptococcus infection was 2.0(0‐6.0) days for HIV‐infected patients, 5.0 (1.5‐8.0) HIV‐uninfected ( p = .008), 2.0 (1.0‐6.0) cryptococcal meningitis (CM) 6.0 (5.0‐8.0) non‐CM <...
Objectives Human JC polyomavirus (JCPyV) infection has an increased risk of developing progressive multifocal leukoencephalopathy (PML). Different JCPyV subtypes differ in the virulence with which they cause PML. Currently, status and subtype distribution patients human immunodeficiency virus-1 (HIV-1) China are still unclear. This study aimed to investigate epidemiology HIV-1-infected China. Methods Urine samples from 137 Zhejiang Province were tested for presence DNA. The detected VP1...
Background The influence of Amphotericin B (AmB) dose and the addition fluconazole (Flu) on AmB + 5-flucytosine (5FC) regimen for cryptococcal meningitis (CM) treatment remain debatable. Method A retrospective study was conducted to compare 44 CM patients treated with 5FC 78 Flu using propensity score matching method. effects dosage, course cerebrospinal fluid (CSF) chemical profile recovery, adverse 90-day mortality were compared between groups. Results No differences in effects, rate...
Pneumocystis pneumonia (PCP) is a common opportunistic infection in acquired immune deficiency syndrome (AIDS) patients that continues to result high mortality rate. To develop better treatment strategy and improve PCP prognosis, cohort study was conducted evaluate the therapeutic potential of echinocandin for AIDS with (AIDS-PCP).The AIDS-PCP were analyzed our retrospective hospitalized The First Affiliated Hospital Zhejiang University during 2013-2018. antifungal effects echinocandins...
Assessing the duration of neutralizing antibodies (nAbs) following SARS-CoV-2 infection or vaccination is critical to evaluate protective immunity and formulate public health strategies. In this study, Ab ELISA (enzyme-linked immunosorbent assay), chemiluminescent microparticle immunoassay (CMIA), as well pseudovirus neutralization test (PVNT) were performed in two cohorts, convalescent patients (CP) from coronavirus disease 2019 (COVID-19) BBIBP-CorV vaccinated population. It was found that...
Indoleamine 2, 3-dioxygenase (IDO) is a key enzyme in the degradation of tryptophan (Trp) to kynurenine (Kyn). We measured IDO activity as Kyn Trp ratio, and investigated whether could be used assess prognosis acquired immune deficiency Sydrome (AIDS) patients with pneumocystis pneumonia (PCP). The concentration were by UPLC-MS/MS plasma samples. A total 49 AIDS-PCP included analysis. Clinical characteristics Kyn/Trp ratio compared between survivors non-survivors. was significantly lower...
To examine the relationship between Pneumocystis jirovecii DNA (PJ-DNA) levels in blood from AIDS-associated pneumonia (AIDS-PCP) and mortality, to correlate mitochondrial large subunit rRNA (mtLSUrRNA) gene polymorphism with we performed a retrospective study including AIDS-PCP patients 2014 2016 one hospital China. PJ-DNA plasma was measured by nested polymerase chain reaction (PCR) of mtLSUrRNA positive specimens further detected level using qPCR. Polymorphisms were observed at two...
Background: Hepatitis B virus (HBV) and the associated cirrhosis are risk factors for cryptococcal meningitis (CM). However, clinical features of co-infection with HBV CM unclear.Methods: Seventy-nine HBV-infected patients 79 HBV-uninfected were enrolled in a case-control matching study from 476 patients. Fibrosis 4 index (FIB4) was used assessment HBV-related fibrosis/cirrhosis. Demographic characteristics, symptoms, routine blood tests, liver function cerebrospinal fluid (CSF) profiles...
Vaccination has proven highly effective against severe acute res-piratory syndrome coronavirus 2 (SARS-CoV-2), but the long-term immunogenicity and functional preserved im-mune responses of vaccines are needed to inform evolving evi-dence-based guidelines for boosting schedules. We enrolled 205 healthcare workers into a cohort study; all had received three doses BBIBP-CorV (China Sinopharm Bio-Beijing Company) inactivated vaccine. assessed SARS-CoV-2 specific binding antibodies, neutralizing...
Vaccination has proven to be highly effective against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but the long-term immunogenicity and functional preserved immune responses of vaccines are needed inform evolving evidence-based guidelines for boosting schedules. We enrolled 205 healthcare workers into a cohort study; all had received three doses BBIBP-CorV (China Sinopharm Bio-Beijing Company, Beijing, China) inactivated vaccine. assessed SARS-CoV-2 specific binding...
To investigate the genetic polymorphisms of mitochondrial large ribosomal subunit (mtLSU)-rRNA, dihydrofolate reductase (DHFR), dihydropteroate synthase (DHPS), cytochrome b (CYB), and superoxide dismutase (SOD) genes its correlation with clinical outcomes Pneumocystis jirovecii pneumonia in acquired immune deficiency(AIDS) patients. Eighty AIDS patients P. that were admitted to our hospital from 2016 2018 included this study. Their demographic information data collected, as well...
Since November 2021, Omicron has emerged as the dominant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant, and its sublineages continue to appear one after another, significantly reducing effectiveness of existing therapeutic neutralizing antibodies (NAbs). It is urgent develop effective NAbs against circulating variants. Here, we isolated receptor binding domain (RBD)-specific single memory B cells via flow cytometry from a COVID-19 convalescent. The antibody variable...
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