Abstract Solanine, a naturally steroidal glycoalkaloid in nightshade (Solanum nigrum Linn.), can inhibit proliferation and induce apoptosis of tumor cells. However, the mechanism solanine‐suppressing prostate cancer cell growth remains to be elucidated. This study investigates inhibition solanine on development vivo cultured human DU 145 vitro. Results show that injection significantly suppresses xenograft athymic nude mice. Solanine regulates protein levels cycle proteins, including Cyclin...

Cervical cancer is a common malignancy in women, with high incidence rate and mortality. Persistent infection of high-risk human papillomavirus (HPV) the most important risk factor for cervical precancerous lesions. Cervicovaginal microbiota (CVM) plays an essential role defense HPV infections prevention subsequent Dominance Lactobacillus key CVM homeostasis, which can be regulated by host, exogenous endogenous factors. Dysbiosis CVM, including altered microbial, metabolic, immune...

Increasing evidence indicates that immunoglobulins are important for the regulation of various cancers including prostate cancer (PCa). However, underlying mechanisms IgG regulated PCa development remain to be further explored. Here, we demonstrated IgG1 heavy chain (IGHG1) was increased in tissues from patients. Inhibition IGHG1 by antibody blocking or genetic knockdown suppressed cell growth and induced cycle arrest ultimate apoptosis. Expression levels c-Myc were positively correlated...

Abstract Perineural invasion (PNI) has emerged as a key pathological feature and be considered poor prognostic factor in cervical cancer. However, the underlying molecular mechanisms are largely unknown. Here, PNI status of 269 squamous cell carcinoma endocervical adenocarcinoma (CESC) samples were quantified by using whole‐slide diagnostic images obtained from The Cancer Genome Atlas. Integrated analyses revealed that was an indicative marker poorer disease‐free survival for CESC patients....

This study explores the protective mechanism of angiotensin (1-7) [Ang-(1-7)] on kidneys by examining its effects renal histomorphology, inflammatory response, oxidative stress, and NF-κB signaling in mice suffering from sepsis-induced acute kidney injury. A injury mouse model was established intracervically injecting lipopolysaccharides (LPS group), followed administration Ang-(1-7) [LPS + group]. The serum levels urea nitrogen, creatinine cystatin. c were measured with an automatic...

Recombinant oncolytic adenovirus offers a novel and promising cancer treatment approach, but its standalone efficacy remains limited. This study investigates combination strategy by co-administering recombinant Adv-loaded silk hydrogel with PD-L1 inhibitor for patients bladder to enhance outcomes. Bladder tissues from mice were collected subjected single-cell sequencing, identifying CRB3 as key gene in malignant cells. Differential expression functional enrichment analyses performed,...

Abstract Objectives To analyze the potential causal-effect of gut microbiota (GM) on neuroblastoma (NB) risk using a Mendelian randomization (MR) study. Methods A two-sample MR study was conducted summary statistics GM from largest available meta-analysis genome-wide association studies by MiBioGen consortium. Pooled for childhood NB were obtained IEU Consortium release data (1627 cases and 3254 controls). Inverse variance-weighted, weighted median, MR-Egger, mod used to examine causal...

Background: Illustrating the pathogenesis of hepatocellular carcinoma (HCC) as well identifying specific biomarkers are great significance.Methods: The original CEL files were obtain from Gene Expression Omnibus, then affymetrix package was used to preprocess files, function DEGs investigated by multiple bioinformatics approach.Finally, typical HCC cell lines and tissue samples using validate role CDC6 in vitro.Bioinformatics software predict potential microRNA CDC6.Luciferase assay verify...

Abstract Objectives This study aims to develop and evaluate the deep learning-based classification model for recognizing pathology of renal tumor from macroscopic cross-section image. Methods A total 467 pathology-confirmed patients who received radical nephrectomy or partial were retrospectively enrolled. The experiment distinguishing malignant benign are conducted followed by performing multi-subtypes models four subtypes tumors, respectively. used same backbone networks which based on...

To examine the expression of CD200 and its receptor (CD200R) in human chorionic villi during first trimester normal pregnancy early spontaneous abortion (ESA).Prospective study.Expression CD200R was determined using streptavidin-peroxidase immunohistochemistry, confocal laser scanning microscopy real-time polymerase chain reaction.Thirty-five women diagnosed with ESA 30 experiencing a healthy medical university hospital China were enrolled this study between 2011 2013.CD200 expression.The...

Cervical cancer is considered one of the diseases with highest mortality among women and limited treatment options. Hydrogen (H₂) inhalation has been reported to have a variety tumor‑suppressive effects, but exact mechanism remains unclear. In present study, HeLa cervical cells HaCaT keratinocytes treated H₂, xenograft mouse model subjected H₂ were established. TUNEL, Cell Counting Kit‑8 Ki67 staining assays used detect cell apoptosis proliferation. Oxidative...

Curcumin (CUR) is a natural compound extracted from turmeric (Curcuma longa), which has been reported to be promising anti-cancer drug in various cancers. However, poor water solubility and low bioavailability posed challenge for clinical application of curcumin. In this work, four CUR prodrugs, namely PEG4000-SS-CUR, PEG1500-SS-CUR, PEG4000-SeSe-CUR PEG1500-SeSe-CUR were synthesized. These prodrugs have high loading content can self-assemble into spherical nanoparticles (NPs) with particle...

To compare perioperative outcomes between robotic single-site surgical technique and conventional laparoendoscopic technique.This was a retrospective cohort study involving 67 patients who received surgery or for the treatment of stage IB1 cervical squamous carcinoma. The radical hysterectomy combined with pelvic lymph node dissections were performed in 32 while 35 patients.The enrolled had been diagnosed mean age (51.63±8.32 years dissection (RSS group) 53.14±8.14 (LESS group), p=0.453);...

Background Interleukin-6 (IL-6) is involved in the activation of several oncogenic pathways prostate cancer. However, its upstream trans-signaling pathway remains largely unknown. This work proposes a mechanistic explanation IL-6's effectors carcinogenesis.

The high costs of operating rooms (ORs) make surgery scheduling decisions critical for hospitals, which refer to resource allocations and determining the start time each surgery. We take into account an important issue that could affect decisions, ie., assistant surgeons. room problem is considered as a two-stage problem. first stage refers allocations, including allocating surgeries ORs assigning surgeons surgeries, while determined in second stage. robustness model tractable way. develop...

Long non-coding RNA (lncRNA) has increasingly been identified as a key regulator in pathologies such cancer. Multiple platforms were used for comprehensive analysis of ovarian cancer to identify molecular subgroups. However, lncRNA and its role mapping the subpopulation are still largely unknown. RNA-sequencing clinical characteristics acquired from The Cancer Genome Atlas database (TCGA). A total 52 lncRNAs aberrant immune specific We redefined two different subtypes, C1(188) C2(184...

Objective: This study aims to identify differentially expressed genes (DEGs) in neuroblastoma (NB) through comprehensive bioinformatics analysis and machine learning techniques. We seek elucidate these DEGs' biological functions associated signaling pathways. Furthermore, our objective extends predicting upstream microRNAs (miRNAs) relevant transcription factors of pivotal genes, with the ultimate goal guiding clinical diagnostics informing future treatment strategies for Neuroblastoma....

Neuroblastoma (NB) is the most common extracranial solid tumor in children, and AURKA gene encodes a protein kinase involved cell cycle regulation that plays an oncogenic role variety of human cancers. The aim this study was to validate biological function prognostic significance NB using basic experiments bioinformatics.

Objective: To examine the cellular distribution and expression of CD200 its receptor 1 (CD200R1) in human deciduas first-trimester pregnant women with spontaneous early abortion (SEA) normal pregnancy, to explore their role etiology SEA.Subjects methods: Thirty-five at 6–10-week gestation SA 30 similar gestational age a healthy pregnancy were recruited. Expression CD200R1 was determined using immunohistochemistry, confocal laser scanning microscope, Western blot, real-time PCR...