Although resveratrol (RES) is barely detectable in the plasma and tissues upon oral consumption, collective evidence reveals that RES presents various bioactivities vivo, including anti-inflammation anti-cancer. This paradox necessitates further research on profiling characterizing biotransformation of RES, as its metabolites may contribute profound biological effects. After 4-week administration, 11 were identified quantified mice by HPLC-MS/MS, dihydro-resveratrol (DHR), lunularin (LUN),...

Abstract Background Development of plant-based products faces challenges like raw material standardization and time-consuming functionality measurements. Fourier Transform Infrared (FTIR) spectroscopy provides a quick, non-destructive way to analyze protein molecular characteristics. Objective This study explored the classification capability FTIR in analyzing five plant isolates—soy, mung bean, pea, fava lentil—and assessed its predictive ability for functional property measurement such as...

Nanosized titanium dioxide (TiO2) particles are commonly present in TiO2 food additives (E171) and have been associated with potential adverse effects on health. However, little knowledge is available regarding the interactions between nanoparticles (NPs) other components, such as flavonoids. In this study, we aim to study molecular anatase NPs three structurally closely related polymethoxyflavones (PMFs, flavonoids found citrus fruits), namely, 3',4'-didemethylnobiletin (DDN),...

This study is to investigate the surface dissipation and foliar penetration of acetamiprid following application live tea plants in presence absence two adjuvants (Alligare 90® Peptoil®). SERS mapping protocol with a self-assembled AuNP mirror as substrates was utilized for situ real time pesticide analysis. The analysis showed that Alligare had lowest rate (34%) compared alone (60%) or Peptoil® (44%), indicating addition more effective reducing than Peptoil®. also investigated into leaves...

Understanding the role of adjuvants in pesticide persistence is crucial to develop effective formulations and manage residues fresh produce. This study investigated impact a commercial non-ionic surfactant product containing alkylphenol ethoxylates (APEOs) on thiabendazole apple spinach surfaces against 30 kg m

5-Demethyltangeretin (5DT) is a unique polymethoxyflavone mainly found in the peel of citrus, and have shown potent suppressive effects on multiple human cancer cells. Biotransformation plays critical role biological activities dietary bioactive components because their metabolites may exert significant bioactivities. In present study, metabolic fate 5DT mouse gastrointestinal (GI) tract after long-term oral intake anti-cancer its major metabolite were determined. It was that underwent...

Abstract Age-grading mosquitoes are significant because only older competent to transmit pathogens humans. However, we lack effective tools do so, especially at the critical point where become a risk In this study, demonstrated capability of using surface-enhanced Raman spectroscopy and artificial neural networks accurately age-grade field-aged low-generation (F2) female Aedes aegypti held under ambient conditions (error was 1.9 chronological days, in range 0–22 days). When degree days were...

Backgroud: (-)-Epigallocatechin-3-gallate (EGCG), the major component of green tea, is well documented to induce apoptosis and cell cycle arrest in cancer by targeting multiple signal transduction pathways. However, EGCG extremely unstable general culture conditions rapidly degraded. So, what extent or which degradation products play a role anti-tumor still unknown. In this study, we evaluated effect different treatments on HCT116 cells.Design: MTT assay was applied inhibitory cells. Cell...

Abstract Swab sampling is a common method for recovering microbes on various environmental surfaces. Its successful application specific target depends the proper swab and following detection assay. Herein, we evaluated critical factors influencing surface sampling, aiming to achieve optimal quantification performance of optical bacterial cells stainless-steel Our results showed recovery rate Salmonella enterica (SE1045) from 10×10 cm2 reached up 92.71±2.19% when using ammonia...

HSC70 has been identified as a direct protein target of nobiletin (NBT), and the interaction between NBT triggers cancer cell death.

Recent advancements in voice conversion and text-to-speech technologies enable the creation of natural-sounding speech, posing challenges to automatic speaker verification systems. In response, research into spoofing countermeasures has intensified safeguard ASV systems from these threats. While advanced can detect known types attacks, their effectiveness diminishes against unknown attacks which have not appeared training set. this work, address challenge determining optimal baseline best...

5‐demethylnobiletin (5‐DN), a citrus‐derived polymethoxyflavone, has many beneficial bioactivities. However, its tissue distribution and metabolism remain unclear, which limits better utilization as nutraceutical ingredient. In this study, CD‐1 mice were fed with 5‐DN in diet for 20 weeks, then the levels of major metabolites i.e. 5, 3'‐didemethylnobiletin (M1), 4'‐didemethylnobiletin (M2) 3', 4'‐tridemethylnobiletin (M3) different tissues determined by HPLC. Our result showed that was...

Accumulating studies have suggested that combination of cancer chemopreventive agents may produce enhanced protective effects against carcinogenesis than each individual agent alone. The efficacy by the can also lower required dose for single in combination, which unwanted side‐effects possibly caused use high agents. Herein, we investigated nobiletin (NBT, a citrus polymethoxyflavone) with atorvastatin (ATST, lipid‐lowering drug) an AOM‐induced colon rat model. results showed oral...

Heat shock proteins (HSPs) is a highly conserved family of molecular chaperones that act to facilitate the proper folding multiple proteins, which allows these achieve their functional conformation. HSPs are overexpressed in many human cancers and implicated tumor cell proliferation, differentiation, invasion metastasis. Our results have demonstrated nobiletin (NBT), major citrus flavonoid, can directly bind HSP70 HSP90 colon cancer cells, provide rationale determine role anticancer effects...

Nobiletin is a major bioactive flavonoid found in citrus fruits that has been shown to have many bioactivities such as anti-cancer and anti-inflammation effects. However, the underlying molecular mechanism of these poorly understood. We hypothesize nobiletin may exert its diverse biological effects by interacting with specific target proteins. was derivatized so it can be covalently bound immobilized sepharose. Whole cell lysates human colon cancer cells were subject affinity chromatography...

Nobiletin is a unique flavonoid mainly found in citrus fruits, and has been reported to inhibit colon carcinogenesis multiple rodent models. However, the direct molecular targets of nobiletin are unknown. Heat shock protein 70 (HSP70) contributes cancer cell survival resistance chemotherapies, thereby inhibition HSP70 promising strategy chemoprevention. Using affinity chromatography, we demonstrated that bound at its ATP‐binding domains human cells specifically inhibited ATPase activity....

5-demethyltangeretin (5DT) is a unique polymethoxyflavone isolated from citrus peels, and showed potent inhibitory effects on various human cancer cells. Biotransformation of dietary bioactive components plays critical roles in their biological activities because they can be transformed to metabolites with significant bioactivities. In this study, we determined the metabolic fate 5DT mice bioactivities its major metabolite. Our results that underwent extensive biotransformation fed 5DT,...

TiO 2 nanoparticles are commonly presented in food additives (E171), and have been associated with potential adverse effects on health. has a specific surface reactivity bind bidentate ligands from the environment. Flavonoids widely found fruits vegetables, can act as electro‐donating agents for nanoparticles. Knowledge molecular interactions between components such flavonoid compounds will facilitate understanding of fate gastrointestinal tract. In this study, we utilized surface‐enhanced...

Pesticides are often applied with adjuvants to facilitate the stability and functionality of AIs in agricultural practice. The objective this study is investigate role a common non-ionic surfactant, alkylphenol ethoxylate (APEO), on surface-enhanced Raman spectroscopic (SERS) analysis pesticides as well its impact pesticide persistence produce surfaces. wettability evaporation rate two (thiabendazole phosmet) mixed APEO were determined respectively correct unit concentration. SERS gold...