A5100363139- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Cervical Cancer and HPV Research
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Aquaculture disease management and microbiota
- Reproductive Biology and Fertility
- Identification and Quantification in Food
- Genomics and Chromatin Dynamics
- Insect Resistance and Genetics
- Hepatitis B Virus Studies
- Ovarian function and disorders
- Epigenetics and DNA Methylation
- RNA regulation and disease
- Cancer Genomics and Diagnostics
- Plant Gene Expression Analysis
- Endoplasmic Reticulum Stress and Disease
- Peroxisome Proliferator-Activated Receptors
- Bioinformatics and Genomic Networks
- Peptidase Inhibition and Analysis
- Microbial Metabolic Engineering and Bioproduction
- Reproductive System and Pregnancy
- Gynecological conditions and treatments
- Cancer-related Molecular Pathways
Shanghai University of Traditional Chinese Medicine
2025
Jiangsu Academy of Agricultural Sciences
2022-2025
Jiangsu University
2025
Fujian Medical University
2024
Henan Normal University
2023-2024
Peking University
2024
Peking Union Medical College Hospital
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Johns Hopkins University
2014-2022
Second Affiliated Hospital of Soochow University
2022
Hepatocellular carcinoma (HCC) is a leading cause of death worldwide. Using cDNA microarrays to characterize patterns gene expression in HCC, we found consistent differences between the HCC compared with those seen nontumor liver tissues. The were also readily distinguished from associated tumors metastatic liver. global intrinsic each tumor sufficiently distinctive that multiple nodules same patient could usually be recognized and all others large sample set on basis their alone. are...
Alternate forms of the PolII transcription initiation machinery have been proposed to play a role in selective activation cell-type-specific gene expression programs during cellular differentiation. The cannonball (can) Drosophila encodes homolog TBP-associated factor (dTAF5) protein expressed only spermatocytes, where it is required for normal genes spermatid We show that primary spermatocytes also express four additional tissue-specific TAFs: nht (homolog dTAF4), mia dTAF6), sa dTAF8) and...
Abstract The polyketide natural product borrelidin displays antibacterial, antifungal, antimalarial, anticancer, insecticidal and herbicidal activities through the selective inhibition of threonyl-tRNA synthetase (ThrRS). How simultaneously attenuates bacterial growth suppresses a variety infections in plants animals is not known. Here we show, using X-ray crystal structures functional analyses, that single molecule occupies four distinct subsites within catalytic domain human ThrRSs. These...
ERA forms a unique family of GTPase. It is widely conserved and essential in bacteria. functions cell cycle control by coupling division with growth rate. homologues also are found eukaryotes. Here we report the crystal structure from Escherichia coli . The has been determined at 2.4-Å resolution. reveals two-domain arrangement molecule: an N-terminal domain that resembles p21 Ras C-terminal unique. Structure-based topological search C fails to reveal any meaningful match, although sequence...
The identification of human body fluids or tissues through mRNA-based profiling is very useful for forensic investigations. Previous studies have shown mRNA biomarkers are effective to identify the origin biological samples. In this study, we selected 16 tissue specific evaluate their specificities and sensitivities identification, including porphobilinogen deaminase (PBGD), hemoglobin beta (HBB) Glycophorin A (GLY) circulatory blood, protamine 2 (PRM2) transglutaminase 4 (TGM4) semen, mucin...
Soybean (Glycine max) is one of the most important industrial and oilseed crops; however, yield threatened by invasion various pathogens. stem root rot, caused Phytophthora sojae, a destructive disease that significantly damages soybean production worldwide. C2H2 zinc finger protein (C2H2-ZFP) large transcription factor family in plants plays crucial roles stress response hormone signal transduction. Given its importance, we analyzed expression patterns C2H2-ZFP genes to P. sojae infection...
Studies on the epidemiological characteristics, treatment strategies and prognosis of nasal paranasal sinus cancer are still relatively limited. This study analyzed age-adjusted incidence rates from 1975 to 2020 using SEER database data. We conducted an in-depth examination patients diagnosed between 2004 2015 with SEER*Stat software. A retrospective Fujian Provincial Cancer Hospital (2013–2020) provided external validation set. Multiple imputation methods in R were used address missing...
2-Methoxyphenol (2MP) solutes form weak complexes with toluene solvent molecules. The are unusual in that the 2MP hydroxyl has an intramolecular hydrogen bond and simultaneously forms intermolecular other aromatic solvents. In equilibrated solute-solvent solution, there exists approximately same concentration of 2MP-toluene complex free 2MP. very fast formation dissociation (chemical exchange) this type three-centered were observed real time under thermal equilibrium conditions...
Congenital lipoid adrenal hyperplasia (lipoid CAH) is the most severe form of CAH, eventually destroying all and gonadal steroidogenesis. Lipoid CAH caused by mutations in steroidogenic acute regulatory protein (StAR), which facilitates entry cholesterol into mitochondria to initiate Patients with typically present a salt-losing crisis first 2 months life, although presentation as late 10 partial retention StAR activity has been reported. We describe eight patients from six Saudi Arabian...
Maintaining the proximity of centrosomes to nuclei is important in several cellular contexts, and LINC complexes formed by SUN KASH proteins are crucial this process. Here, we characterize presumed Drosophila ortholog mammalian protein, sperm-associated antigen 4 (Spag4, previously named Giacomo), demonstrate that Spag4 required for centriole nuclear attachment during spermatogenesis. Production spag4 mRNA limited testis, protein shows a dynamic pattern association with germline nuclei,...
Cervical cancer is one of the most common malignant tumors in gynecology.Deploying HIVID, a cost-effective technique to detect HPV integration sites, our team had studied characteristics integrations cervical exfoliated cells.Our results indicated that both sample proportion and number gradually increased following development lesion.Meanwhile, data also revealed there were recurrent genes integrated by cells.Collectively, breakpoints highly enriched intron promoter regions.Intriguingly,...
To prevent potential errors in protein synthesis, some aminoacyl-transfer RNA (tRNA) synthetases have evolved editing mechanisms to hydrolyze misactivated amino acids (pre-transfer editing) or misacylated tRNAs (post-transfer editing). Class Ia leucyl-tRNA synthetase (LeuRS) may misactivate various natural and non-protein then mischarge tRNA Leu . It is known that the fidelity of prokaryotic LeuRS depends on multiple pathways clear incorrect intermediates products every step aminoacylation...
Abstract Taxanes are important drugs in the treatment of ovarian and other cancers, but their efficacy is limited by intrinsic acquired drug resistance. Expression multidrug transporter P‐glycoprotein, encoded MDR1 ( ABCB1 ) gene, one causes clinical resistance to taxanes. To study mechanisms MDR 1 activation related taxanes, we established 11 multidrug‐resistant variants from six cancer cell lines continuous exposure either paclitaxel or docetaxel. We profiled gene expression copy number...
One of the major challenges in development prostate cancer prognostic biomarkers is cellular heterogeneity tissue samples. We developed an objective Cluster-Correlation (CC) analysis to identify gene expression changes various cell types that are associated with progression. In Cluster step, samples were clustered (unsupervised) based on values each through a mixture model combined multiple linear regression which cell-type percent data used for decomposition. Correlation Chi-square test was...
Minus-one programmed ribosomal frameshifting (−1 PRF) allows the precise maintenance of ratio between viral proteins and is involved in regulation half-lives cellular mRNAs. activated by several stimulatory elements such as a heptameric slippery sequence (X XXY YYZ) an mRNA secondary structure (hairpin or pseudoknot) that positioned 2–8 nucleotides downstream from site. Upon −1 RF, reading frame shifted normal zero to with decoded XXX YYY Z instead X YYZ. Our research group has developed...