A5075506418- Biochemical effects in animals
- Protein Hydrolysis and Bioactive Peptides
- Food Allergy and Anaphylaxis Research
- Collagen: Extraction and Characterization
- Meat and Animal Product Quality
- Allergic Rhinitis and Sensitization
- Stress Responses and Cortisol
- Contact Dermatitis and Allergies
- Free Radicals and Antioxidants
- Adipose Tissue and Metabolism
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Muscle metabolism and nutrition
- Agricultural safety and regulations
- Gut microbiota and health
- Neuropeptides and Animal Physiology
- Food Supply Chain Traceability
- Antioxidant Activity and Oxidative Stress
- Identification and Quantification in Food
- Pesticide Residue Analysis and Safety
- Proteins in Food Systems
- Amino Acid Enzymes and Metabolism
- Renin-Angiotensin System Studies
- Exercise and Physiological Responses
- Probiotics and Fermented Foods
Osaka Seikei University
2023
Conestoga Meat Packers (Canada)
2000-2013
Higashihiroshima Medical Center
2007-2013
Hiroshima University
2007-2013
In this study, collagen extracted from chicken legs (which are the yellow keratin parts containing a nail) was hydrolyzed with various enzymes, and angiotensin I-converting enzyme (ACE)-inhibitory activity of each hydrolysate determined. The by treatment an Aspergillus species-derived had highest (IC 50 = 260 microg/mL). fraction obtained ultrafiltration molecular-weight cutoff 3000 Da (low fraction) stronger 130 microg/mL) than fractionated one. This further HPLC, peptides in high...
Royal jelly (RJ) has several physiological effects and is widely used in commercial medical products health foods. We examined the of RJ supplementation on serum lipoprotein metabolism humans. Fifteen volunteers were divided into an intake group (n=7) a control (n=8). The took 6 g per day for 4 wk. Their total cholesterol (TC) low-density (LDL) decreased significantly compared with those (p<0.05). There no significant differences high-density (HDL) or triglyceride concentrations. Moreover,...
Royal jelly peptides (RJPx) isolated from hydrolysates of water-soluble royal proteins prepared with protease P exhibited significantly stronger hydroxyl radical-scavenging activity (p<0.001), and antioxidant against lipid peroxidation (LPO, p<0.001), than did protein (WSRJP) in vitro. We also investigated the vivo RJPx ferric nitrilotriacetate (Fe-NTA)-induced LPO. Male Wistar rats were divided into a control group (Group C), an Fe-NTA Fe), Fe+R). Rats Group Fe+R fed (2 g/kg body weight)...
Effects of chicken collagen hydrolysate (CCH) on atherogenesis in apolipoprotein E-deficient C57BL/6.KOR-ApoE(shl) mice were investigated this paper. The fed either a normal CE-2 diet (control group) or containing 10% CCH (CCH for 12 wk. Compared with that the control, amount total plasma cholesterol, hepatic cholesterol and triglycerides group was reduced by 14.4, 24.7 42.8%, respectively. Histological analysis results showed abundance diffuse lipid droplets fat vacuoles decreased group....
Gly-Ala-Hyp-Gly-Leu-Hyp-Gly-Pro (GAXGLXGP, X: Hyp), an octapeptide contained in chicken collagen hydrolysate, inhibits angiotensin I-converting enzyme activity vitro. Intestinal Caco-2 and bovine aortic endothelial cells (BAECs) were used to investigate whether the transported GAXGLXGP improves vascular function. When was added apical side of monolayers, intact form released basolateral without incorporation into cells. This transport energy-independent but associated with tight junction...
<i>Background:</i> Recently, some strains of lactic acid bacteria (LAB) have been reported to prevent the development atopic dermatitis and improve allergic symptoms, especially in young children. However, mechanisms involved these effects are not fully understood. Intestinal microbiota play critical roles host immune recognized regulated by through intestinal epithelial cells (IECs). We thus hypothesized that LAB influence system activation IECs. To begin testing this...
Chicken collagen hydrolysate was given to 15 mildly hypertensive subjects for 4 weeks. Blood pressure significantly decreased by 11.8 mmHg (P<0.01). A reduction in plasma renin activity observed blood test after intake. colony assay of endothelial progenitor cells samples from non-smokers revealed an approximately 30% increase the number colonies.
We investigated the effects of a chicken collagen hydrolysate (CCH) on circulation system in humans. A total 58 subjects with either mild hypertension (systolic blood pressure (SBP) 140-159 mmHg or diastolic (DBP) 90-99 mmHg) high-normal (SBP 130-139 DBP 85-89 were assigned to two groups, one involving placebo and other, test food (including CCH 2.9 g/d). The parameters related each subject's monitored over study period 18 weeks. Δbrachial-ankle pulse wave velocity (baPWV), an indicator...
We previously reported that chicken collagen hydrolysate (CCH) has strong angiotensin I converting enzyme (ACE) inhibitory activity and antihypertensive effects on spontaneously hypertensive rats. Here, we investigated the chronic therapy of CCH blood pressure vascular relaxation in a cardiovascular damage model Wistar-Kyoto rats induced by N-nitro-l-arginine methyl ester (L-NAME). Following co-treatment with for 4 weeks, increment systolic was suppressed significantly. At 8 vasorelaxation...
Intestinal permeability to size-classified fish collagen peptides (FCPs) was examined using Caco-2 monolayers. Minimum-sized FCPs were most efficiently transported across the Permeability depended on peptide size. It independent of H(+)-coupled transport system but associated with tight junction permeability, suggesting that paracellular pathway is responsible for transepithelial peptides.
The purpose of this study was to investigate the effects long-term chicken breast extract (CBEX) supplementation, a rich source carnosine and anserine, on relatively high intensity endurance performance. Sixteen healthy male subjects were divided into CBEX group (n=8) placebo (n=8). orally administered 200 ml drink which contained 4g anserine per day for 30 days. 200ml same taste no anserine. Before after ingestion period, performed three sessions consecutive exercise (first session: 30-min...
Carnosine (β-alanyl-L-histidine) and its derivative anserine (β-alanyl-1-methyl-L-histidine) are antioxidants putative neurotransmitters in the brain. These dipeptides rich commercially available supplement chicken breast extract (CBEX). To clarify effects of CBEX on brain, we examined whether single oral administration (20 ml/kg) affects brain dipeptide free amino acid concentrations male Wistar rats. significantly time-dependently increased carnosine levels plasma (at 120 min after...
Various stages of the food chain, from production to processing distribution, can impact safety. The concept "food defense" has emerged as a countermeasure against intentional contamination with foreign substances. Although knowledge hygiene is common among consumers, there are currently no reports consumer surveys on defense.This study aims investigate awareness defense and We analyzed results focusing how consumers behave when they find abnormalities in further our promoting...
降圧作用を有する鶏コラーゲン加水分解物(C-COP)を健常人に摂取させ,C-COPの血中動態およびアンジオテンシン変換酵素(ACE)阻害活性との関連性について検討した.体重60kgあたり25gのC-COPをヒトに単回摂取させた場合,血中に遊離ヒドロキプロリン(Hyp)とともに,ペプチド型のHypも確認された.血中のペプチド型Hyp量は,2時間後(91.1μmol/L)に最大となった.摂取2時間後の血中からPro-Hyp, Leu-Hyp, Pro-Hyp-Gly, Phe-Hyp, Ala-Hyp, Hyp-Gly, Glu-Hyp-Gly, Ala-Hyp-Gly, Ser-Hyp-GlyのHyp含むペプチドが確認された.血中ペプチドは,ACEに対する阻害活性を有しており,Ala-Hyp(IC50 : 0.177mmol/L)の活性がもっとも高かった.以上より,C-COPの降圧作用は,血中ペプチドによるACE阻害が作用機序であると考えられた.
Several amino acids have effects on mental function, including sedative, antidepressant-like and anxiolytic-like effects. However, the influence of integrated acid nutrition as protein constituents function remains unclear. Therefore, purpose present study was to compare chicken, pork beef extracts brain monoamine metabolism in mice. Changes levels their turnover rates were induced by different sources. In particular, chicken group showed highest norepinephrine hippocampus hypothalamus,...
Novel peptides derived from chicken collagen hydrolysate (CCH) were confirmed to have a vasoprotective effect after ingestion and absorption vessels; however, their endovascular mechanism of action required clarification. Therefore, using human umbilical vein endothelial cells (HUVECs), in which inflammation had been induced by tumor necrosis factor-α (TNF-α), we investigated the CCH-derived inhibited vascular inflammatory response. Expression soluble intercellular adhesion molecule-1...
特定原材料である小麦タンパク質測定のELISA法の確立のために10機関による検証評価試験を行った．グリアジンおよび小麦タンパク質を測定する2種類ELISA法とも同時再現性はおおむねCV値10%以下と良好であった．10機関で小麦標準溶液を添加した5食品の各食品抽出液を分析した際の平均回収率は，2種類ELISA法ともおおむね40%以上であり，併行再現性の相対標準偏差は，それぞれ16～26.9%，3.7～36.2%であり，室間再現性の相対標準偏差はそれぞれ21.6～38.5%，29.7～53.8%であり，ELISA測定としては実用上支障がないと考えられた．小麦エライザキットのプレートに結合した抗体量を増加した結果，回収率が低かったシリアルで添加回収率が改善された．2種類ELISA法の検出限界は，測定溶液の濃度で1 ng/mLであった．
本研究では,鶏コラーゲンペプチド中に含まれ,強いACE阻害活性を有するオクタペプチドのSHRへの血圧降下作用および消化酵素による分解性について検討した.(1) オクタペプチドをSHRに4.5mg/kg体重で単回投与した結果,SHRの収縮期血圧値を有意に低下させた.(2) 試験管レベルでオクタペプチドは,ペプシンおよび,トリプシン/キモトリプシン,小腸上皮酵素に対して難消化性を示し,そのACE阻害活性は維持されていた.以上の結果から,鶏コラーゲンペプチドに含まれるオクタペプチドは,in vitroでのACE阻害活性を持つだけでなく,SHRの血圧降下作用を示すことが確認された.
Inter-laboratory evaluation studies were conducted for the notified ELISA methods allergic substances (Egg). Standard extracts of egg spiked in sausage, sauce, cookie, bread and cereal at a level 5-20 ng/mL as sample solution analyzed replicate 10 laboratories. Coefficients variation (CVs) all three using an Egg Protein ovalbumin Kit (ovalbumin kit), ovomucoid (ovomucoid kit) FASTKIT kit (Egg mostly less than 10%. Mean recoveries standard extract over 40% methods. Repeatability relative...
特定原材料であるそばの通知試験法ELISA法の評価のために10機関による検証評価試験を行った．そばタンパク質を測定する2種ELISA法とも併行再現性はおおむねCV値10%以下と良好であった．10機関でそば標準溶液を添加した3食品の各食品抽出液を分析した際の平均回収率は，2種ELISA法とも40%以上であり，併行再現性の相対標準偏差は，それぞれ6.8～78.5%，5.0～33.9%であり，室間再現性の相対標準偏差はそれぞれ11.9～69.5%，16.5～34.1%であり，ELISA測定としては実用上支障がないと考えられた．2種ELISA法の検出限界は，測定溶液の濃度で1 ng/mLであった．
We investigated the postprandial thermic effect of chicken and its mechanisms in rats. A diet showed a strong after consumption, removal fat induced more rapid stronger thermogenesis. Although thermogenesis by purified protein was also strong, reaction not so remarkable rise peripheral temperatures observed. Defatted activated thyroid hormone system up-regulated rate-limiting enzyme genes glucose metabolism tricarboxylic acid (TCA) cycle liver. Moreover, mRNA expression hepatic lipid...
We compared the effects of purified meat proteins on postprandial thermogenesis and secretion responsiveness to thyroid hormones (THs) in rats. Body temperatures at 2 h after feeding were significantly higher chicken mutton protein groups than other groups, these seem have a strong thermogenic effect. There no significant differences plasma TH concentrations among but levels TH-responsive Spot 14 liver brown adipose tissue groups. Levels malic enzyme 1 group except for group. Furthermore,...