A5049471924- Cell death mechanisms and regulation
- Gut microbiota and health
- PARP inhibition in cancer therapy
- Autophagy in Disease and Therapy
- Acne and Rosacea Treatments and Effects
- Antibiotic Resistance in Bacteria
- MicroRNA in disease regulation
- Endoplasmic Reticulum Stress and Disease
- Ear Surgery and Otitis Media
- Extracellular vesicles in disease
- Pharmacological Effects of Natural Compounds
- Vibrio bacteria research studies
- Autoimmune and Inflammatory Disorders
- Advanced Glycation End Products research
- interferon and immune responses
- Sphingolipid Metabolism and Signaling
- Nasal Surgery and Airway Studies
- Genetics, Bioinformatics, and Biomedical Research
- Curcumin's Biomedical Applications
- Phonocardiography and Auscultation Techniques
- Infective Endocarditis Diagnosis and Management
- Lipid metabolism and biosynthesis
- RNA Interference and Gene Delivery
- Dermatology and Skin Diseases
- Acute Lymphoblastic Leukemia research
Semmelweis University
2007-2025
Hungarian Research Network
2024
Paracelsus Medical University
2017
National Institute of Oncology
2011-2013
Graz University of Technology
2012
Extracellular vesicles (EVs) released by mesenchymal stromal cells (MSCs) may contribute to biological processes such as tissue regeneration, immunomodulation and neuroprotection. Evaluation of their therapeutic potential application in future clinical trials demands thorough characterization EV content production under defined medium conditions, devoid xenogenic substances serum-derived vesicles. Addressing the apparent need for a growth medium, we have developed formulation based on pooled...
For a long time necrosis was thought to be an uncontrolled process but evidences recently have revealed that can also occur in regulated manner. Necroptosis, type of programmed is defined as death receptor-initiated under caspase-compromised conditions. The requires the kinase activity receptor-interacting protein 1 and 3 (RIPK1 RIPK3) mixed lineage domain-like (MLKL), substrate RIPK3. further downstream events remain elusive. We applied known inhibitors characterize contributing enzymes...
The regenerative and immunomodulatory activity of mesenchymal stromal cells (MSCs) is partially mediated by secreted vesicular factors. Extracellular vesicles (EVs) exocytosed MSCs are gaining increased attention as prospective non-cellular therapeutics for a variety diseases. However, the lack suitable in vitro assays to monitor therapeutic potential EVs currently restricts their application clinical studies. We have evaluated dual immunomodulation potency assay that reproducibly reports...
Brain tumors are frequently diagnosed diseases in which etiology and progression largely depend on mutations genetic factors. Additionally, recent reports document that the microbiome may influence tumor growth, microenvironment, response to therapy. Our goal was examine extent bacterial composition—microbiota—and fungal composition—mycobiota—characteristic of its microenvironment correlate with composition gut blood microbiota mycobiota five randomly selected brain patients. The tumor,...
Abstract Background The interactions of oxidized low-density lipoprotein (LDL) and macrophages are hallmarks in the development atherosclerosis. biological activities modified particle these cells due to content lipid oxidation products apolipoprotein modification by phospholipids. Results It was aim this study determine role short-chain phospholipids as components LDL cultured macrophages. For purpose we investigated effects following on cell viability apoptosis: 1-palmitoyl-2-glutaroyl- sn...
The important regulatory role of the guanine-quadruplex (GQ) structure, present in nuclease hypersensitive element (NHE) III1 region human c-myc (h c-myc) gene's promoter, regulation transcription that gene has been documented. Here we evidences, nuclear poly(ADP-ribose)polymerase-1 PARP-1) protein participates h expression through its interaction with this GQ characterized by binding assays, fluorescence energy transfer (FRET) experiments and affinity pull-down vitro, chromatin...
Previous research confirmed gut dysbiosis and translocation of selected intestinal bacteria into the vessel wall in abdominal aortic aneurysm patients. We studied stool, blood, thrombus microbiomes 21 patients using 16S rRNA sequencing. Our goals were to determine: 1. whether microbiome characteristic an differs from that a healthy vessel, 2. detectable are translocated through bloodstream, 3. enzymatic activity can contribute destruction wall. The abundance Acinetobacter, Burkholderia,...
A carbapenem-resistant
The number of bioinformatics tools and resources that support molecular cell biology approaches is continuously expanding. Moreover, systems network analyses are accompanied more by integrated methods. Traditional information-centered university teaching methods often fail, as (1) it impossible to cover all existing in the frame a single course, (2) large segment current bioinformation can become obsolete few years. Signaling offers an excellent example for tools, both focused complex at...
It is now generally accepted that the success of antitumor therapy can be impaired by concurrent antibiotic therapy, presence certain bacteria, and elevated defensin levels around tumor tissue. The aim our current investigation was to identify underlying changes in microbiome tissue induced different antibiotics, as well duration this modification. tissues significantly from healthy volunteers. Comparing only samples, no significant difference confirmed between untreated group treated with...
Darier disease (DD) is a rare autosomal dominant genodermatosis characterized by erythematous papules and plaques mainly involving sebaceous areas, such as the face, chest back. Skin microbiome plays an essential role in maintaining skin homeostasis. A disturbed may contribute to exacerbation of DD. We investigated bacterial composition two predilectional sites DD patients healthy individuals. also measured deeper layers, where diversity was significantly reduced compared superficial layer...
Several research groups have confirmed that in the pathogenesis of chronic inflammatory skin disorder rosacea, composition and fecal microbiome affected patients differs from healthy individuals. We studied stool, blood microbiomes rosacea control using 16S rRNA sequencing. Our goals were to determine 1. whether characteristics differ individuals, 2. change experienced on can be by alterations stool through mediation 3. metabolic activity changed skin, or play a role rosacea. The differed...
Although otitis media with effusion (OME) is a common cause of hearing impairment in children, little known about its microbiological background. We aimed to analyse the microbiome middle ear fluid (MEF) OME identify microbiological, demographic and environmental factors that may potentially influence microbiome. In addition, we compare results conventional culture techniques PCR-based methods.Thirty-nine samples from children were investigated using 16S rRNA sequencing. Bioinformatic...
The asymptomatic gastrointestinal colonization of multidrug-resistant (MDR) bacteria can lead to difficult-to-treat infections. We investigated the role host factors influencing in an orogastrical murine infection model using a CTX-M-15- and OXA-162-producing
An external ventricular drain (EVD) is used to release elevated intracranial pressure by draining cerebrospinal fluid (CSF) from the brain's ventricles. The establishment of an EVD one most commonly performed neurosurgical procedures treat in patients. Nevertheless, infections are very frequent complications. Identifying risk factors for EVD-related a key improving patient safety and outcomes.
The recognition of poly ADP-ribose transferase-1 (PARP-1) as an ATP sensor receiving this energy source by way a specific adenylate kinase wire (AK) from mitochondrial synthase (F0F1), and directly regulating cellular mRNA DNA synthesis, was the first step towards identification effect PARP-1 that is fundamental significance. molecular target AK-ATP Arg 34 Zn finger I PARP-1, which also site for cation-π interactions π-electron donors. We now identify receptor second active center...
Abstract The borderline between necrosis and apoptosis is indistinct, but that types of cell death important because may lead to local inflammation, whereas usually does not. In certain autoimmune disorders, inhibition crucial, since macromolecules released from the dead cells accelerate processes. We have used various inhibitors block induced by 4HPR [N‐(4‐hydroxyphenil)‐retinamide] BL41 U937 lines. VD‐FMK, a general caspase inhibitor, inhibited DNA fragmentation 4HPR, not PI (propidium...