A5107483314- Mast cells and histamine
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Food Allergy and Anaphylaxis Research
- Myasthenia Gravis and Thymoma
- Peripheral Neuropathies and Disorders
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Allergic Rhinitis and Sensitization
- Monoclonal and Polyclonal Antibodies Research
- Protein Kinase Regulation and GTPase Signaling
- Urticaria and Related Conditions
- Acute Ischemic Stroke Management
- Respiratory and Cough-Related Research
- Immunodeficiency and Autoimmune Disorders
- T-cell and B-cell Immunology
- Renal function and acid-base balance
- Cell Adhesion Molecules Research
- Tissue Engineering and Regenerative Medicine
- Muscle and Compartmental Disorders
- Renal and Vascular Pathologies
- Neurological Disease Mechanisms and Treatments
- Analytic and geometric function theory
- Congenital gastrointestinal and neural anomalies
- Cancer, Stress, Anesthesia, and Immune Response
- HER2/EGFR in Cancer Research
Nagoya University
2005-2024
La Jolla Institute for Immunology
1996-2023
University of California, San Diego
2020-2023
Japanese Red Cross Nagoya Daini Hospital
2017-2019
La Jolla Alcohol Research
2019
Okazaki City Hospital
2011
Gifu Prefectural General Medical Center
2011
Aichi Medical University
2011
Gifu Pharmaceutical University
2005
University of Washington
2003
Bruton tyrosine kinase (EC 2.7.1.112) [Btk, encoded by Btk in mice and BTK humans (formerly known as atk, BPK, or emb)], which is variously mutated chromosome X-linked agammaglobulinemia patients immunodeficient (xid) mice, has the pleckstrin homology (PH) domain at its amino terminus. The PH of expressed a bacterial fusion protein directly interacts with C mast cell lysates. Evidence was obtained that physically associated intact murine cells well. Both Ca(2+)-dependent (alpha, beta I, II)...
Muscle-specific receptor tyrosine kinase (MuSK) antibody-positive myasthenia gravis (MG) accounts for 5%-15% of autoimmune MG. MuSK mediates the agrin-signaling pathway and also anchors collagenic tail subunit (ColQ) acetylcholinesterase (AChE). The exact molecular target MuSK-immunoglobulin G (IgG), however, remains elusive. As acetylcholine (AChR) deficiency is typically mild as cholinesterase inhibitors are generally ineffective, we asked if MuSK-IgG interferes with binding ColQ to...
MuSK antibody-positive myasthenia gravis (MuSK-MG) accounts for 5 to 15% of autoimmune MG. and LRP4 are coreceptors agrin in the signaling pathway that causes clustering acetylcholine receptor (AChR). also anchors acetylcholinesterase (AChE)/collagen Q (ColQ) complex synaptic basal lamina. We previously reported anti-MuSK antibodies (MuSK-IgG) block binding ColQ cause partial endplate AChE deficiency mice. here analyzed physiological significance this by MuSK-IgG. In vitro plate-binding...
Cytoplasmic aggregation of TDP-43 in neurons is a pathological feature common to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). We demonstrate that the IκB kinase (IKK) complex promotes degradation cytoplasmic through proteasomes. While IKKβ major factor degradation, IKKα acts as cofactor, NEMO functions scaffold for recruitment IKK complex. Furthermore, we identified IKKβ-induced phosphorylation sites found at Thr8 Ser92 important reduction by IKK. was...
Btk plays crucial roles in the differentiation and activation of B myeloid cells. Despite drastic reductions other Ig isotypes, paradoxically high IgE responses have been known btk mutant mice. Here we show that –/– dendritic cells exhibit a more mature phenotype stronger vitro vivo T cell-stimulatory ability than wild-type Increased were induced by adoptive transfer into Consistent with cells, mice exhibited enhanced inflammation Th2-driven asthma Th1-driven contact sensitivity experiments....
<ns4:p>Mast cells are innate immune that intersect with the adaptive immunity and play a crucial role in initiation of allergic reactions host defense against certain parasites venoms. When activated an allergen- immunoglobulin E (IgE)-dependent manner, these secrete large variety allergenic mediators pre-stored secretory granules or<ns4:italic>de novo</ns4:italic>–synthesized. Traditionally, studies have predominantly focused on understanding this mechanism mast cell activation regulation....
Functional T lymphocyte activation requires concurrent stimulation of the TCR complex and an accessory molecule, most frequently CD28. We have previously demonstrated that TEC family tyrosine kinase EMT/ITK/TSK (EMT) is activated following cross-linking demonstrate herein CD3 component also leads to EMT as indicated by a rapid transient increase in phosphorylation activity anti-EMT immunoprecipitates. However, although CD28 results marked production cell growth factor IL-2, it does not...
Protein kinase C (PKC) and Syk protein tyrosine play critical roles in immune cell activation including that through the high-affinity IgE receptor, FcεRI. Mechanisms by which PKC leads to of Ras, a family GTPases essential for activation, have been elusive. We present evidence Tyr-662 Tyr-658 PKCβI PKCα, respectively, are phosphorylated membrane compartment FcεRI-stimulated mast cells. These phosphorylations require prior autophosphorylation adjacent serine residues (Ser-661 Ser-657,...
Food allergy occurs due to IgE- and mast cell-dependent intestinal inflammation. Previously, we showed that histamine-releasing factor (HRF), a multifunctional protein secreted during allergy, interacts with subset of IgE molecules the HRF dimer activates cells in an HRF-reactive IgE-dependent manner. In this study, investigated whether plays any role food allergy. Specifically, determined prophylactic therapeutic administration inhibitors block HRF-IgE interactions reduces incidence...
Mouse body temperature measurement is of paramount importance for investigating allergies and anaphylactic symptoms. Rectal probes readings common, they have been proven to be accurate invaluable in this regard. However, method requires the mice anesthetized order insert probe without injury animal. This limits ability observe other phenotypes mouse simultaneously. In investigate while measuring temperatures, rectal are not ideal, another desired. Here, we introduce a noninvasive that...
Bioactive immunomodulatory biomaterials have shown promise for influencing the immune response to promote tissue repair and regeneration. Macrophages T cells been associated with this response; however, other cell types traditionally overlooked. In study, we investigated role of mast in regulation decellularized biomaterial scaffolds using a subcutaneous implant model. cell-deficient mice, there was dysregulation expected M1 M2 macrophage transition typically induced by scaffold....
Crohn's disease is a chronic, debilitating, inflammatory bowel disease. Here, we report critical role of phospholipase C-β3 (PLC-β3) in intestinal homeostasis. In PLC-β3-deficient mice, exposure to oral dextran sodium sulfate induced lethality and severe inflammation the small intestine. The was due PLC-β3 deficiency multiple non-hematopoietic cell types. resulted reduced Wnt/β-catenin signaling, which essential for homeostasis regeneration epithelium. regulated pathway epithelial cells...
Bone marrow-derived mouse mast cells contain phospholipase C-gamma 1 (PLC-gamma 1), which is phosphorylated at tyrosine residues upon cross-linking of cell-bound IgE antibodies with multivalent antigen. It was found that immune complexes formed from digitonin lysates the by monoclonal anti-PLC-gamma contained protein-tyrosine kinase (PTK), PLC-gamma in vitro. The activity coprecipitated 1-anti-PLC-gamma markedly increased when cell were obtained immediately after antigen challenge. results...
Histamine-releasing factor (HRF) is a multifunctional protein with fundamental intracellular functions controlling cell survival and proliferation. HRF also secreted during allergic reactions promotes IgE-mediated activation of mast cells basophils. In this study, we investigated secretion its relevance to airway inflammation. monomers were constitutively from BEAS-2B human bronchial epithelial (HBECs) converted oligomers over the course culture. Stimulation house dust mite (HDM) extract...
Acute exacerbation is the major cause of asthma morbidity, mortality, and health-care costs. Respiratory viral infections, particularly rhinovirus (RV) are associated with majority exacerbations. The risk for bronchoconstriction RV allergic sensitization type 2 airway inflammation. efficacy humanized anti-IgE monoclonal antibody omalizumab in treating reducing frequency severity RV-induced well-known. Despite these clinical data, mechanistic details omalizumab's effects on have not been...
Research Articles| July 03 2009 Studies on the Relationship between Allergic Reaction and Nucleic Acids of Organs Subject Area: Immunology Allergy Y. Kawakami; Kawakami Medical Department Tokyo University Search for other works by this author on: This Site PubMed Google Scholar T. Shimizu; Shimizu Watanabe; Watanabe Katsuta; Katsuta A. Ashida International Archives Applied (1956) 8 (5): 252–261. https://doi.org/10.1159/000228295 Article history Published Online: Content Tools Views Icon...