A5018843980- Cellular transport and secretion
- Genetics, Aging, and Longevity in Model Organisms
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- Photosynthetic Processes and Mechanisms
- Ion channel regulation and function
- Molecular Junctions and Nanostructures
- Electrochemical Analysis and Applications
- Prion Diseases and Protein Misfolding
- DNA and Nucleic Acid Chemistry
- Calcium signaling and nucleotide metabolism
- Retinal Development and Disorders
- Genetic diversity and population structure
- Receptor Mechanisms and Signaling
- Electrochemical sensors and biosensors
- CRISPR and Genetic Engineering
- Erythrocyte Function and Pathophysiology
- Corporate Social Responsibility Disclosure
- Inflammation biomarkers and pathways
- Wildlife Ecology and Conservation
- Biochemical Acid Research Studies
- Alzheimer's disease research and treatments
- Biochemical and Structural Characterization
- Gold and Silver Nanoparticles Synthesis and Applications
- Surface Chemistry and Catalysis
Huazhong University of Science and Technology
2009-2025
Sichuan University
2021
West China Hospital of Sichuan University
2021
Taizhou First People's Hospital
2012
Boston Children's Hospital
2010
Harvard University
2010
Wuhan University
2002-2005
Xiamen University
2002
Fluorescent dextrans are commonly used as macropinocytic probes to study the properties of endocytic cargoes; however, effect size on mechanisms has not been carefully analyzed. By using chemical and siRNA inhibition individual pathways, we evaluated internalization two dextrans, Dex10 (dextran 10 kDa) Dex70 70 kDa), in mammalian HeLa cells Caenorhabditis elegans coelomocytes. We revealed that enters these cell types predominantly via clathrin- dynamin-independent amiloride-sensitive...
Background The notion that AP-2 clathrin adaptor is an essential component of endocytic coat appears to conflict with recent observations substantial depletion, using RNA interference synthesis subunits, fails block uptake certain ligands known internalize through a clathrin-based pathway. Methodology/Principal Findings We report here the use in vivo imaging data obtained by spinning-disk confocal microscopy study formation clathrin-coated structures at plasma membranes BSC1 and HeLa cells...
Carbon fiber nanoelectrodes (tip diameter = ca. 100 nm) have been first used to monitor real-time dopamine release from single living vesicles of rat pheochromocytoma (PC12) cells. The experiments show that active and inactive sites exist on the surface cells, spatial distributions differentiated even in same zone. It is demonstrated multiple can sequentially at site cell surface, which possibly plays main role PC12
The endosomal system maintains cellular homeostasis by coordinating multiple vesicular trafficking events, and the retromer complex plays a critical role in cargo recognition sorting. Here, we demonstrate an essential for small GTPase RAB21 regulating retromer-mediated recycling of glucose transporter SLC2A1/GLUT1 macroautophagy/autophagy. depletion mis-sorts SLC2A1 to lysosomes affects uptake, thereby activating AMPK-ULK1 pathway increase autophagic flux. also increases lysosome function....
A significant hallmark of Alzheimer's disease is the formation senile plaques in brain due to unbalanced levels amyloid-beta (Aβ). However, although how Aβ produced from amyloid precursor proteins well understood, little known regarding clearance and metabolism various aggregates brain. Similarly, astrocytes internalize degrade Aβ, are play an important role plaque maintenance clearance. The objective this study investigate cellular mechanisms that mediate internalization soluble monomeric...
P4-ATPases are phospholipid flippases responsible for the transbilayer lipid asymmetry. ATP8A1, a P4-ATPase family member, has been reported to be involved in phosphatidylserine (PS) translocation at trans-Golgi network, early endosomes and recycling endosomes. However, possible roles of PS on late endosomes/lysosomes pathway how they regulated remain elucidated. This study showed enrichment ATP8A1 Rab7-positive endosomal compartments, that primarily flips from luminal leaflet cytosolic but...
Significance Understanding how clathrin-independent endocytic (CIE) cargo proteins are sorted into and transported within endosomes has attracted growing interests. Here, in Caenorhabditis elegans intestine, a well-established genetic system for deciphering trafficking multicellular organisms, we report direct visualization of dynamic endosomal tubules as carriers basolateral recycling certain CIE cargoes. We unveil the coordination Rab GTPase RAB-10, exocyst component SEC-10, microtubule...
The immobilization of thiol-derivatized DNA on a Au (111) single crystal surface by self-assembly has been investigated electrochemical scanning tunneling microscopy (EC-STM). Continuous potential-dependent orientation changes double-stranded oligodeoxynucleotides (ODN) have observed in certain potential range from 200 to 600 mV (versus SCE). It is suggested that the duplexes stand straight gold at potentials negative zero charge (pzc) and then lay down when shifts positively. These results...
Double-stranded DNA(poly(dA)30·poly(dT)30) -modified gold electrodes, prepared by air-drying/adsorption method, have been investigated various techniques, including cyclic voltammetry (CV), quartz crystal microbalance (QCM), electrochemical scanning tunneling microscopy (EC−STM), and surface-enhanced Raman scattering spectroscopy (SERS). CV QCM results show that an average surface coverage of (7.5 ± 0.2) × 10-12 mol cm-2 was obtained for poly(dA)30·poly(dT)30-modified close to the value a...
The South China tiger (Panthera tigris amoyensis) is endemic to and also the most critically endangered subspecies of living tigers. It considered extinct in wild only about 150 individuals survive captivity date, whose genetic heritage, however, ambiguous controversial. Here, we conducted an explicit assessment 92 studbook-registered tigers from 14 captive facilities using a subspecies-diagnostic system context comparison with other voucher specimens evaluate ancestry level distinctiveness...
Abstract Endocytosis and postendocytic sorting of G-protein-coupled receptors (GPCRs) is important for the regulation both their cell surface density signaling profile. Unlike mechanisms clathrin-dependent endocytosis (CDE), underlying control GPCR by clathrin-independent (CIE) remain largely unknown. Among muscarinic acetylcholine (mAChRs), M4 mAChR undergoes CDE recycling, whereas M2 internalized through CIE targeted to lysosomes. Here we investigated trafficking based on a comparative...
Many cells utilize a GTP-dependent pathway to trigger exocytosis in addition Ca(2+)-triggered exocytosis. However, little is known about the mechanism by which GTP triggers independent of Ca(2+). We used dual-color evanescent field microscopy compare motion and fusion large dense core vesicles stimulated either mastoparan (Mas) Ca(2+)-free conditions or high K(+) presence demonstrate that Mas hardly effective triggering predocked but predominantly mobilizes cytosolic vesicles. In contrast,...
Early endosomes are the sorting hub on endocytic pathway, wherein nexins (SNXs) play important roles for formation of distinct membranous microdomains with different functions. Tubular mediate recycling clathrin-independent (CIE) cargoes back toward plasma membrane. However, molecular mechanism underlying tubule is still poorly understood. Here we screened effect ARF-6-associated CIE endosomal tubules all SNX members in Caenorhabditis elegans ( C . ). We identified SNX-3 as an essential...
There has been a consensus that actin plays an important role in scission of the clathrin-coated pits (CCPs) besides large GTPase Dynamin metazoan cells. However, recruitment, regulation, and functional interdependence during this process remain inadequately understood. Here, based on small-scale screen vivo live-imaging techniques, we identified novel set molecules underlying CCP multicellular organism Caenorhabditis elegans. We found loss Wiskott−Aldrich syndrome protein (WASP)-interacting...
茁-Spectrinisamajorcomponentofthemembraneskeleton,astructurefoundattheplasmamembraneofmostanimalcells.C.elegans genomeencodesonlyone geneof 茁-spectrinsubunit(茁 -G), unc鄄70.Besidesstabilizingplasmamembranesandgenerating cellpolarity, 茁-spectrinisalsoreportedtomediatethesecretarypathway.Inthepresentstudies,itwasdemonstratedthatUNC-70is involvedinthedensecorevesicle(DCV)exocytosisin C.elegans neurons.Itwasfoundthat unc鄄70 dominantallelemutantsexhibited reducedpeptiderelease invivo