A5027890976- RNA modifications and cancer
- interferon and immune responses
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Advanced Optimization Algorithms Research
- Circular RNAs in diseases
- Cancer Immunotherapy and Biomarkers
- Esophageal Cancer Research and Treatment
- Sparse and Compressive Sensing Techniques
- Cancer, Hypoxia, and Metabolism
- Iterative Methods for Nonlinear Equations
- Protein Degradation and Inhibitors
- Peptidase Inhibition and Analysis
- Optimization and Variational Analysis
- Advanced Proteomics Techniques and Applications
- RNA Research and Splicing
- Machine Learning in Bioinformatics
- RNA and protein synthesis mechanisms
- Bioinformatics and Genomic Networks
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- NF-κB Signaling Pathways
- Immune Cell Function and Interaction
- Cancer Mechanisms and Therapy
Guizhou University
2020-2025
Sun Yat-sen University
2016-2025
Sun Yat-sen University Cancer Center
2016-2025
State Key Laboratory of Oncology in South China
2018-2025
Chinese PLA General Hospital
2022-2025
Hainan University
2025
Hubei University of Automotive Technology
2025
Third Affiliated Hospital of Southern Medical University
2021-2024
Xidian University
2017-2023
Ocean University of China
2023
Recent high-throughput techniques have generated a flood of biological data in all aspects. The transformation and visualization multi-dimensional numerical gene or protein expression single heatmap can provide concise but comprehensive presentation molecular dynamics under different conditions. In this work, we developed an easy-to-use tool named HemI (Heat map Illustrator), which visualize either heatmaps. Additionally, the heatmaps be recolored, rescaled rotated customized manner....
Small ubiquitin-like modifiers (SUMOs) regulate a variety of cellular processes through two distinct mechanisms, including covalent sumoylation and non-covalent SUMO interaction. The complexity regulations has greatly hampered the large-scale identification substrates or interaction partners on proteome-wide level. In this work, we developed new tool called GPS-SUMO for prediction both sites SUMO-interaction motifs (SIMs) in proteins. To obtain an accurate performance, generation group-based...
Abstract Background Long noncoding RNAs (lncRNAs) play nonnegligible roles in the epigenetic regulation of cancer cells. This study aimed to identify a specific lncRNA that promotes colorectal (CRC) progression and could be potential therapeutic target. Methods We screened highly expressed lncRNAs human CRC samples compared with their matched adjacent normal tissues. The proteins interact LINRIS (Long Intergenic Noncoding RNA for IGF2BP2 Stability) were confirmed by pull-down...
Discussion | Our findings extend prior research in other settings [1][2][3] to support visible, innovative actions by oncology meeting organizers, including women's networking centers and facilitation of childcare services, as steps ensure full participation all those who might contribute or benefit at conferences. 4he primary limitations this study are that the data were self-reported respondents (55% response rate) not be representative population.We did measure training institution...
Circulating tumor DNA methylation–based signatures are potential biomarkers for early diagnosis and prognosis of colorectal cancer.
Abstract N 6 -methyladenosine (m A) modification is an important mechanism in miRNA processing and maturation, but the role of its aberrant regulation human diseases remained unclear. Here, we demonstrate that oncogenic primary microRNA-25 (miR-25) pancreatic duct epithelial cells can be excessively maturated by cigarette smoke condensate (CSC) via enhanced m A mediated NF-κB associated protein (NKAP). This catalyzed overexpressed methyltransferase-like 3 (METTL3) due to hypomethylation...
Chemotherapy is the preferred treatment for advanced stage gastric cancer (GC) patients and chemotherapy resistance major obstacle to effective therapy. Increasing evidence suggests that mesenchymal stem cells (MSCs) make important contributions development of drug resistance. However, underlying mechanism remains elusive. In this study, we discovered abundant MSCs in tumor tissues predicted a poor prognosis GC patients. promoted stemness chemoresistance through fatty acid oxidation (FAO)...
As the most important post-translational modification of proteins, phosphorylation plays essential roles in all aspects biological processes. Besides experimental approaches, computational prediction phosphorylated proteins with their kinase-specific sites has also emerged as a popular strategy, for its low-cost, fast-speed and convenience. In this work, we developed predictor GPS 2.1 (Group-based Prediction System), novel but simple approach motif length selection (MLS). By approach,...
Metabolic enzymes have an indispensable role in metabolic reprogramming, and their aberrant expression or activity has been associated with chemosensitivity. Hence, targeting remains attractive approach for treating tumors. However, the influence regulation of cysteine desulfurase (NFS1), a rate-limiting enzyme iron-sulfur (Fe-S) cluster biogenesis, colorectal cancer (CRC) remain elusive. Here, using vivo gene-based clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9...
Ferroptosis is a type of cell death related to cancer; however, the characteristics ferroptosis in cancers are still uncertain. Based on data The Cancer Genome Atlas, we found that most regulator genes (FRGs) were differentially expressed tumors, somatic copy number alterations (SCNA) and DNA methylation contributed their aberrant expression. We established potential index (FPI) reveal functional roles noticed FPI was higher tumors than normal tissues associated with subtypes clinical...
Breast cancer is one of the most common malignancies and major cause cancer-related death in women. Although importance PIWI-interacting RNAs (piRNAs) has been increasingly recognized, few studies have explored functional mechanism piRNAs breast development progression.We examined top 20 highly expressed based on analysis TCGA data two patient cohorts to test roles cancer. The effects piRNA-36,712 malignant phenotypes chemosensitivity cells were detected vitro vivo. MS2-RIP reporter gene...
Abstract Tumor cells often reprogram their metabolism for rapid proliferation. The roles of long noncoding RNAs (lncRNAs) in remodeling and the underlying mechanisms remain elusive. Through screening, we found that lncRNA Actin Gamma 1 Pseudogene ( AGPG ) is required increased glycolysis activity cell proliferation esophageal squamous carcinoma (ESCC). Mechanistically, binds to stabilizes 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3). By preventing APC/C-mediated...
Abstract Fat mass and obesity-associated protein (FTO), an N6-methyladenosine (m 6 A) demethylase, participates in tumor progression metastasis many malignancies, but its role colorectal cancer (CRC) is still unclear. Here, we found that FTO levels, not RNA were downregulated CRC tissues. Reduced expression was correlated with a high recurrence rate poor prognosis resectable patients. Moreover, demonstrated hypoxia restrained expression, mainly due to increase ubiquitin-mediated degradation....
Abstract 5-Methylcytosine (m 5 C) is a posttranscriptional RNA modification participating in many critical bioprocesses, but its functions human cancer remain unclear. Here, by detecting the transcriptome-wide m C profiling esophageal squamous cell carcinoma (ESCC), we showed increased methylation ESCC tumors due to overexpressed methyltransferase NSUN2. Aberrant expression of NSUN2 was positively regulated E2F Transcription Factor 1 (E2F1). High levels predicted poor survival patients....
Sex is known to be an important factor in the incidence, progression, and outcome of cancer. A better understanding underlying mechanisms could help improve cancer prevention treatment. Here, we demonstrated a crucial role antitumor immunity sex differences Consistent with observations human cancers, male mice showed accelerated tumor progression compared females, but these were not observed immunodeficient mice. Androgen signaling suppressed T-cell against males. Mechanistically,...
Background and objective Gastric cancer (GC) remains a prevalent preventable disease, yet accurate early diagnostic methods are lacking. Exosome non-coding RNAs (ncRNAs), type of liquid biopsy, have emerged as promising biomarkers for various tumours. This study aimed to identify serum exosome ncRNA feature enhancing GC diagnosis. Designs Serum exosomes from patients with (n=37) healthy donors (n=20) were characterised using RNA sequencing, potential validated through quantitative reverse...
As one of the most important and ubiquitous post-translational modifications (PTMs) proteins, S-nitrosylation plays roles in a variety biological processes, including regulation cellular dynamics plasticity. Identification S-nitrosylated substrates with their exact sites is crucial for understanding molecular mechanisms S-nitrosylation. In contrast labor-intensive time-consuming experimental approaches, prediction using computational methods could provide convenience increased speed. this...
In eukaryotes, hundreds of protein kinases (PKs) specifically and precisely modify thousands substrates at specific amino acid residues to faithfully orchestrate numerous biological processes, reversibly determine the cellular dynamics plasticity. Although over 100,000 phosphorylation sites (p-sites) have been experimentally identified from phosphoproteomic studies, regulatory PKs for most these still remain be characterized. Here, we present a novel software package iGPS prediction in vivo...
We reported an integrated database of Compendium Protein Lysine Modifications (CPLM; http://cplm.biocuckoo.org) for protein lysine modifications (PLMs), which occur at active ε-amino groups specific residues in proteins and are critical orchestrating various biological processes. The CPLM was updated from our previously developed Acetylation (CPLA), contained 7151 acetylation sites 3311 proteins. Here, we manually collected experimentally identified substrates 12 types PLMs, including...
Overcoming oxidative stress is a critical step for tumor progression; however, the underlying mechanisms in colorectal cancer (CRC) remain unclear.We investigated nicotinamide adenine dinucleotide (phosphate) (NAD(P))-dependent enzyme methylene tetrahydrofolate dehydrogenase 2 (MTHFD2) expression, clinical relevance, redox modification, and molecular using CRC cells tissues (n = 462 paired samples). The antitumor effects of MTHFD2 inhibitor LY345899 on tumorigenesis metastasis were evaluated...
Objective To monitor trastuzumab resistance and determine the underlying mechanisms for limited response rate rapid emergence of HER2+ metastatic gastric cancer (mGC). Design Targeted sequencing 416 clinically relevant genes was performed in 78 paired plasma tissue biopsy samples to plasma-tissue concordance. Then, we longitudinal analyses 97 serial collected from 24 patients who were track during treatment validated identified candidate genes. Results The results targeted sequencing-based...
Although PIWI-interacting RNAs (piRNAs) have recently been linked to human diseases, their roles and functions in malignancies remain unclear. This study aimed investigate the significance of some piRNAs colorectal cancer (CRC). Methods: We first analyzed expression profile CRC using TCGA GEO databases. The top 20 highly expressed were selected tested our tumor non-tumor tissue samples. then examined relevance significantly differentially piRNA outcomes 218 patients receiving postoperative...