A5083392976- Protein Kinase Regulation and GTPase Signaling
- FOXO transcription factor regulation
- Cancer-related Molecular Pathways
- Cytokine Signaling Pathways and Interactions
- Circular RNAs in diseases
- Histone Deacetylase Inhibitors Research
- PI3K/AKT/mTOR signaling in cancer
- NF-κB Signaling Pathways
- Cell death mechanisms and regulation
- Wnt/β-catenin signaling in development and cancer
- Cancer-related molecular mechanisms research
- COVID-19 Clinical Research Studies
- RNA modifications and cancer
- Multiple Myeloma Research and Treatments
- Biomarkers in Disease Mechanisms
- Protist diversity and phylogeny
- Respiratory viral infections research
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Chemotherapy-induced organ toxicity mitigation
- Infectious Encephalopathies and Encephalitis
- IL-33, ST2, and ILC Pathways
- RNA Research and Splicing
- Inflammasome and immune disorders
Zhongshan Hospital
2013-2024
Fudan University
2013-2024
Institute of Hematology & Blood Diseases Hospital
2019
The First People’s Hospital of Lianyungang
2013
Xian Yang Central Hospital
2013
Tongji Hospital
2006-2010
Huazhong University of Science and Technology
2006-2010
Dear editor, In late December 2019, a novel contagious pneumonia named coronavirus disease 2019 (COVID-19) has broken out in Wuhan, China.1 On January 30, 2020, World Health Organization (WHO) declared COVID-19 as Public Emergency of International Concern. March 11, WHO characterized pandemic.2, 3 Much research work been done for hospitalized patients, mainly clinical characteristics.4 However, few studies have reported the post-discharge follow-up status, especially mental health status...
Abstract Background Chronic obstructive pulmonary disease (COPD) remains a prevalent chronic airway inflammatory disease. Circular RNAs (circRNAs) are associated with inflammation regulation; therefore, we examined distinct effects of circRNA FOXO3 (circFOXO3) against pneumonic processes in COPD. Methods We first quantified and localized circFOXO3 mouse lung epithelial cell line MLE12 by quantitative reverse-transcription PCR situ hybridization. Next, was suppressed therapeutic...
Glibenclamide (Gli) is a widely employed drug in the treatment of type 2 diabetes and many lines evidence have described its anti-tumor effects some neoplasms. The aim present study was to investigate effect Gli on apoptosis human hepatocellular carcinoma (HCC) cells analyze underlying pathway involved this action. Two HCC cell lines, HepG-2 Huh7 were used as models. We found that significantly inhibited viability, induced significant cell-cycle arrest G2/M-phase both cells. further verified...
Lung cancer is a devastating with high morbidity and mortality. Ubiquitin‑specific protease (USP) type of deubiquitinating enzyme (DUB) that has been implicated in numerous cancers, including colorectal, myeloma breast. In the present study, expression USP51 was determined lung cell line A549 cisplatin (also known as DDP)‑resistant strain A549/DDP. The zinc‑finger E‑box binding homeobox 1 (ZEB1), transcriptional repressor, also examined. effects knockdown or overexpression on proliferation...
Highlights•First large-scale real-world study of oral antivirals in treating COVID-19 China.•S/R reduces the risk related hospitalization, especially elderly.•During XBB circulation, S/R shows faster symptom resolution and viral clearance.SummaryBackgroundSimnotrelvir has demonstrated potent anti-viral activity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In a Phase II/III study, Simnotrelvir plus ritonavir (S/R, co-packaged) shortened time to symptoms adult...
Abnormal hypertrophy and hyperplasia of airway smooth muscle cells play an important role in remodeling chronic asthma. The authors' previous studies have indicated that protein kinase Cα (PKCα) is involved the proliferation passively sensitized human (HASMCs). However, underlying mechanisms remain unknown. Here, authors examined possible α isoform PKC control cyclin D1 expression, using HASMCs on atopic asthmatic serum as a model system. Cultured were with from patients. Cell was measured...
To explore the effect of cigarette smoke (CS) on development squamous metaplasia in human airway epithelial cells and role MAPK- FoxA2-signaling pathways process.In an vitro study, we treated bronchial cell line BEAS2B with CS extract, followed by treatment ERK inhibitor U0126, JNK SP600125, or p38 SB203580. In vivo, used a CS-induced rat model. After without MAPK inhibitors for 90 days, lung tissues were harvested. p-ERK, p-p38 p-JNK protein levels tissue measured using enzyme-linked...
Background/Aims: The transcription factor CCAAT/enhancer-binding protein α (C/EBPα) is a basic leucine zipper that plays essential roles in tumor progression. Although decreased or absent C/EBPα expression many cancers suggests possible role for as suppressor, the functions of lung adenocarcinoma remain unclear. Methods: Here, levels 26 and para-carcinoma tissue samples were detected by qRT-PCR immunohistochemistry. Cell transwell assays, wound healing assay three-dimensional spheroid...
Background Airway smooth muscle (ASM) is suspected to be a determining factor in the structural change of asthma. However, role protein kinase C α (PKCα) and cyclin D1 involved dysfunction ASM leading asthmatic symptoms not clear. In this study, central PKCα proliferation rats was explored. Methods Thirty-six pathogen-free male Brown Norway (BN) were randomly divided into 2 groups: control groups (group N1, N2 N3) A1, A2, A3). Groups A2 A3 challenged with ovalbumin (OA) for weeks, 4 weeks 8...
Asthma is a chronic disease characterized by reversible airway obstruction, hyperresponsiveness, and inflammation of airways. Th2 cells, one sort CD4+ T lymphocytes, are currently considered to play an important role in the asthma. Meanwhile, number laboratories have clearly established importance Th2-derived cytokine interleukin-4 (IL-4) mediating inflammatory response. Anti-IL-4 therapy might be beneficial treatment Gene represents novel approach for Compared with other virus vectors,...
Objective: To study the phenotype of memory CD4(+) T cells in peripheral blood asthmatics and its relationship with asthma severity. Methods: From Dec 2014 to Aug 2015, thirty-three asthmatics, twenty-six chronic obstructive pulmonary disease (COPD) patients twenty-two healthy volunteers were enrolled Respiratory Clinics Zhongshan Hospital, Fudan University. Peripheral mononuclear (PBMCs) isolated from blood. Cell surface markers (CD45RO, CRTH2, CD62L, CCR7) intracellular protein[interleukin...
Primary ciliary dyskinesia (PCD) is a rare, genetically heterogeneous disorder caused by dysfunction of the cilia and flagella; however, causative genetic defects have not been detected in all patients with PCD. Seven Chinese Han Kartagener syndrome were enrolled onto present study. Transmission electron microscopy (TEM) was performed to evaluate cilial whole‑exome sequencing used analyze relevant variations patients. In two seven PCD, four novel dynein axonemal assembly factor 1 (DNAAF1)...
Objective To construct a siRNA expressing vector targeting FoxM1,in order to investigate the effect of FoxM1 gene in lung cancer and other diseases.Methods A short hairpin DNA fragment was designed synthesized,and then cloned into linearized backbone plasmid pSilencer2.l-U6.The new identified be correct by cutting with restriction enzymes sequencing.Results The shDNA inserted pSilencer 2.1-U6 correctly.Conclusions small interfering RNA expression is constructed successfully. Key words:...
Background: Deregulation of the ubiquitin‐proteasome system (UPS) is linked to pathogenesis and drug resistance Multiple Myeloma (MM). Proteasome inhibitor has provided significant therapeutic advances in treatment MM, however dosage dependent side effects remain a clinical challenge. Our recent research efforts led discovery new agents which can potentially overcome toxicity bortezomib. Aims: To profile anti‐MM activity explore possible mechanisms indirubin‐3’‐monoxime (Id3) mediating....