The phosphoinositide 3-kinase (PI3K)/Akt signalling pathway appears to be a key regulator in cervical carcinogenesis. However, the downstream regulatory mechanism of PI3K/Akt remains largely unknown.The expression miR-196a cancer cell lines and tissues was examined using real-time PCR. effects on cellular proliferation were evaluated by bromodeoxyuridine labelling, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide, colony formation assays luciferase assays.The level markedly...

Acquisition of the chemoresistance to docetaxel (DTX), a microtubule-targeting agent, has been huge obstacle in treatment for metastatic castration-resistant prostate cancer (mCRPC). Recently, strategies targeting mitosis error correction mechanism including chromosomal passenger complex (CPC) were reported reverse resistance anticancer agents. Meanwhile, accumulating evidence indicated important roles circRNAs DTX (PCa). However, whether could regulate chemosensitivity by affecting remains...

The aim of this study was to elucidate the value putative cancer stem cell markers Musashi-1, ALDH1, Sox2, and CD49f in predicting prognosis cervical squamous carcinoma (CSCC). Real-time PCR immunohistochemistry staining performed examine expression archived specimens CSCC patients with postoperative chemotherapy. Kaplan–Meier analysis Cox proportional hazards model were used assess prognostic impact CSC for overall survival (OS) recurrent-free (RFS). data showed that all increased tissues...

Abstract Background Circular RNAs (circRNAs) have been associated with bladder cancer (BC), but the specific underlying molecular mechanism of their association BC development has not fully explored. Methods Levels Circ_0008532, MTGR1 and miR-155-5p/miR-330-5p in cell lines tissues were determined quantitative real-time PCR western blotting assays. In vitro vivo assays performed to investigate function circ_0008532 tumorigenesis cells. The relationships predicted using bioinformatic tools...

Purpose: Nudix Hydrolase 21 (NUDT21) is a crucial mediator involved in alternative polyadenylation (APA), and this molecule has been reported to be tumor suppressor human cancers.However, neither the role NUDT21 plays bladder cancer (BC) nor mechanisms which are have investigated.Methods: Expression levels of BC were evaluated with real-time PCR, western blotting, immunohistochemistry (IHC).In vitro vivo assays performed investigate function tumorigenesis cells.The TOP/FOP flash reporter...

Abstract Cell therapy is a promising strategy for cancer therapy. However, its therapeutic efficiency remains limited due to the complex and immunosuppressive nature of tumor microenvironments. In this study, “cell-chemotherapy” was presented enhance antitumor efficacy. M1-type macrophages, which are immune cells with both immunotherapeutic ability targeting ability, carried sorafenib (SF)-loaded lipid nanoparticles (M1/SLNPs) were developed. macrophages used as tool provide immunotherapy...

The tumor penetration of nanomedicines constitutes a great challenge in the treatment solid tumors, leading to highly compromised therapeutic efficacy nanomedicines. Here, we developed small morph nanoparticles (PDMA) by modifying polyamidoamine (PAMAM) dendrimers with dimethylmaleic anhydride (DMA). PDMA achieved deep via an active, energy-dependent, caveolae-mediated transcytosis, which circumvented obstacles process penetration. remained negatively charged under normal physiological...

(1) Background: Increasing evidence indicates that lipid metabolism may influence the concentration of prostate-specific antigen (PSA). However, association between triglycerides and PSA remains unclear complicated. Hence, we evaluated correlation based on U.S. National Health Nutrition Examination Survey (NHANES) database. (2) Methods: A total 2910 participants out 41,156 fit into our study after conducting screening from 2003 to 2010 NHANES survey. Serum were independent variable study,...

Abstract Combination therapy is a current hot topic in cancer treatment. Multiple synergistic effects elicited by combined drugs are essential improving antitumor activity. Herein, pH‐triggered charge and size dual switchable nanocage co‐loaded with abemaciclib IMD‐0354 (PA/PI‐ND) reported, exhibiting novel triple‐interlocked combination of chemotherapy, immunotherapy, chemoimmunotherapy. The reversal polymer NGR‐poly(ethylene glycol)‐poly( l ‐lysine)‐dimethylmaleic anhydride...

About 10-20% of patients with bladder cancer (BC) progress to muscle-invasive diseases, which the underlying key molecular events have yet be addressed. Here, we identified poly(A) binding protein nuclear 1 (PABPN1), a general factor alternative polyadenylation (APA), was downregulated in BC. Overexpression and knockdown PABPN1 significantly decreased increased BC aggressiveness, respectively. Mechanistically, provide evidence that preference PABPN1-bound signals (PASs) depends on relative...

Centrosomal protein 55 (CEP55) is a cell cycle regulator implicated in development of certain cancers. However, characteristics CEP55 expression and its clinical/prognostic significance are unclear human epithelial ovarian carcinoma (EOC). Therefore, we investigated the clinicopathological patients with EOC role regulating invasion metastasis lines. mRNA levels were detected by quantitative real-time PCR (qRT-PCR), Western blotting, immunohistochemistry (IHC). Potential associations scores...

Src family tyrosine kinase (SFK) activation is associated with ovarian cancer progression. Therefore, SFKs are targets for the development of potential treatments cancer. Dasatinib a inhibitor that SFK activity, and used treatment B cell Abelson lymphomas. At present time, effect dasatinib on not clear. The aim study was to investigate antitumor activity dasatinib, alone in combination paclitaxel, vitro vivo. In study, expression phospho‑Src-Y416 (p‑Src) measured six lines using western...

Leupaxin (LPXN) is a member of the paxillin protein family. Several studies have reported that LPXN regulates cancer development; however, role in bladder remains unknown.The expression cells and tissues was determined by real-time PCR, western blotting, immunohistochemistry, respectively. The biological cell proliferation, invasion, angiogenesis explored both vitro vivo.LPXN elevated lines compared to adjacent non-tumor normal urothelial cells. High correlated with large tumor size,...

ABSTRACT Zinner syndrome (ZS) is a rare anomaly of the Wolffian duct. Here, we present case seminal vesiculectomy and ipsilateral nephroureterectomy using laparoscopic surgery in modified supine position, which was performed 30‐year‐old male patient with ZS. To our knowledge, this first report to describe feasible use approach remove vesicle atrophic kidney single session.

Upregulator of cell proliferation 4 (URG4) has been implicated in the oncogenesis certain cancers. However, correlation between URG4 expression and clinicopathological significance human cancer remains unclear. Therefore, this study investigated its cervical patients. was examined using quantitative PCR (qPCR) western blotting normal epithelial cells, eight matched pairs tissues adjacent noncancerous from same patient. In addition, immunohistochemistry (IHC) used to examine paraffin-embedded...

Bladder cancer (BC) is a common genitourinary malignancy. This study investigated the regulatory effects of an exonic circRNA, circNUDT21, in progression BC. The circNUDT21 level was overexpressed BC tissues and cell lines as compared to normal controls. Overexpression silencing promoted inhibited, respectively, proliferative invasive abilities cells. Mechanistical analysis showed that acted miR-16-1-3p sponge MDM2 potential downstream target miR-16-1-3p. We further verified overexpression...

Alternative polyadenylation (APA) is an important post-transcriptional regulatory mechanism in cancer development and progression. Poly(A) binding protein nuclear 1 (PABPN1) a gene that encodes abundant protein, binds with high affinity to nascent poly(A) tails, crucial for 3'-UTR (3'-untranslated region) APA. Although PABPN1 has been recently reported as dominant master APA regulator clear cell renal carcinoma (ccRCC), the underlying functional remain unclear genes subject regulation...

MicroRNA‑122 (miR‑122) has been reported to be involved in the pathogenesis of several types malignancies; however, its role prostate carcinoma remains unknown. Thus, current study aimed investigate functionality miR‑122 carcinoma. Clinical data 54 patients with who were diagnosed and treated Union Hospital (Wuhan, China) between January 2011 2013 retrospectively analyzed. The expression levels Rho‑associated protein kinase 2 (ROCK2) tumor adjacent healthy tissues patients, as well serum...

“Layer peeling” co-delivery systems provide a novel strategy to realize xenotype cells-targeting delivery and enhance the cancer chemoimmunotherapy effects.