A5007498454- Glycosylation and Glycoproteins Research
- Proteoglycans and glycosaminoglycans research
- Carbohydrate Chemistry and Synthesis
- Vibrio bacteria research studies
- Peptidase Inhibition and Analysis
- Listeria monocytogenes in Food Safety
- Advanced Proteomics Techniques and Applications
- Prostate Cancer Treatment and Research
- Cancer, Lipids, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Prostate Cancer Diagnosis and Treatment
- Endoplasmic Reticulum Stress and Disease
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Monoclonal and Polyclonal Antibodies Research
- Peroxisome Proliferator-Activated Receptors
- Autophagy in Disease and Therapy
- Heat shock proteins research
- RNA Research and Splicing
- Adipose Tissue and Metabolism
- HER2/EGFR in Cancer Research
- Ferroptosis and cancer prognosis
- Analytical Chemistry and Chromatography
- Photochemistry and Electron Transfer Studies
- Chemotherapy-induced organ toxicity mitigation
Peking University Shenzhen Hospital
2020-2025
Chongqing Medical University
2024-2025
Hangzhou Medical College
2024-2025
Zhejiang Provincial People's Hospital
2024-2025
Jinggangshan University
2025
Hong Kong University of Science and Technology
2020-2024
University of Hong Kong - Shenzhen Hospital
2020-2024
Chongqing University
2017-2020
Northwest University
2012-2017
Liaoning University
2011
Fast, sensitive, and simple methods for quantitative analysis of disparities in glycan expression between different biological samples are essential studies protein glycosylation patterns (glycomics) the search disease biomarkers. Relative quantitation glycans based on stable isotope labeling combined with mass spectrometric detection represents an emerging promising technique. However, this technique is undermined by complexity spectra isotope-labeled caused presence multiple metal ion...
// Wenhu Liu 1, 2, 3 , Jinxia Chang 4 Mingwei 2 Jiangbei Yuan 1 Jinqiang Zhang Jun Qin 5, 6 Xuefeng Xia and Yi Wang School of Pharmaceutical Sciences Innovative Drug Research Center, Chongqing University, 401331, China State Key Laboratory Proteomics, National Center for Protein Sciences, Beijing Proteome 102206, Pharmacy, North Sichuan Medical College, Nanchong 637007, Basic 5 Verna Marrs McLean Department Biochemistry Molecular Biology, Baylor College Medicine, Houston, Texas 77030, USA...
Resistance to trastuzumab, which specifically target HER2-positive breast and gastric cancer, can develop ultimately in cancer patients. However, the underlying mechanisms of resistance have not been fully elucidated. Here, we established trastuzumab-resistant MKN45 NCI N87 sublines from their parental cells. The resistant cells exhibited characteristics epithelial-mesenchymal transition (EMT) acquired higher migratory invasive capacities. To exploit activated pathways new strategies...
Abstract Background Primary prostate cancer with metastasis has a poor prognosis, so assessing its risk of is essential. Methods This study combined comprehensive ultrasound features tissue proteomic analysis to obtain biomarkers and practical diagnostic image that signify metastasis. Results In this study, 17 benign prostatic hyperplasia (BPH), primary without (PPCWOM), (PPCWM) were comprehensively analyzed the corresponding proteome data perform weighted gene co-expression network (WGCNA),...
Cellular membrane proteins are a critical part of the host defense mechanisms against infection and intracellular survival Listeria monocytogenes The complex spatiotemporal regulation bacterial by various has been challenging to study. Here, using mass spectrometry analyses, we depicted dynamic expression landscape upon L. in dendritic cells. We showed that Dynein light chain 1 (Dynll1) formed persistent with mitochondrial cytochrome oxidase Cox4i1, which is disturbed pathogen insult....
Glycosylation is one of the most common eukaryotic post-translational modifications, and aberrant glycosylation has been linked to many diseases. However, glycome analysis a significantly challenging task. Although several lines evidence have indicated that protein defective in Alzheimer's disease (AD), only few studies focused on AD glycomics. The etiology unclear there are no effective disease-modifying treatments for AD. In this study, we found object recognition memory, passive...
Qualitative and quantitative studies of glycosylation patterns various biologically important proteins represent a key field for the understanding their complex structure-function relationships. However, analysis glycoprotein glycans is usually undermined by tedious sample processing steps prior to detection, including deproteination, desalting removal some other non-glycan impurities, which results in considerable loss increased difficulty analysis. Herein we report facile versatile method...
Abstract Through digital rectal examinations (DRE) and routine prostate‐specific antigen (PSA) screening, early prostate cancer (PC) treatment has become possible. However, PC is a complex heterogeneous disease. In vivo, cells can invade adjacent tissues metastasize to other resulting in hard cures. Therefore, the key improving patients’ survival time preventing cells’ metastasis. We used mass spectrometry profile primary patients with versus without metastatic PC. named these two groups of...
The aim of this paper was to investigate the effects curcumin on proliferation,migration,invasion and apoptosis human gastric cancer cells explore potential mechanisms. SGC7901,MKN45 NCI N87 lines were cultured under different concentrations curcumin( 2. 5,5,10,20,40,80 160 μmol·L~(-1)) at time points( 12,24,48 72 h),and effect cell proliferation detected by CCK-8 assay. migration invasiveness determined wound healing Transwell assays,the rate assessed flow cytometry,the expression...
N-glycans play important roles in various pathophysiological processes and can be used as clinical diagnosis markers. However, plasma change their significance the setting of hypercholesterolemia, a major risk factor for atherosclerosis, is unknown. Here, we collected from both hypercholesterolemic patients cholesterol-fed rabbits, determined changes whole-plasma N-glycan profile by electrospray ionization mass spectrometry. We found that rabbits showed dramatic glycan profile. Compared with...
Macrophages are sentinels in the organism which can resist and destroy various bacteria through direct phagocytosis. Here, we reported that expression level of mitochondrial ribosomal protein S35 (Mrps35) continued to decrease over infection time after Listeria monocytogenes (L. monocytogenes) infected macrophages. Our results indicated knockdown Mrps35 increased load L. This result supported played crucial roles infection. Moreover, performed comprehensive proteomics analyze differentially...
Lipid droplets (LDs) are multifunctional organelles consisting of a central compartment non-polar lipids shielded from the cytoplasm by phospholipid monolayer. The excessive accumulation LDs in cells is closely related to development and progression many diseases humans animals, such as liver-related cardiovascular diseases. Thus, regulating size abundance necessary maintain metabolic homeostasis. This study found that lipopolysaccharide (LPS) stimulation reduced content mouse liver. We...
Abstract Background: Long non-coding RNA colon cancer-associated transcript 1 (CCAT1) is involved in transforming multiple cancers into malignant cancer types. Previous studies underlining the mechanisms of functions CCAT1 primarily focused on its decoy for miRNAs (micro RNAs). However, regulatory mechanism CCAT1–protein interaction associated with tumor metastasis still largely unknown. The present study aimed to identify proteome-wide partners and explored mediated metastasis. Methods:...
The interaction between 3, 5-dihydroxytoluene and bovine serum albumin (BSA) had been investigated by fluorescence spectroscopy. By the analysis of spectrum intensity, it was observed that a strong ability to quench intrinsic BSA through static quenching procedure. binding constant number sites were determined at 298 K based on results. thermodynamic parameters such as enthalpy change (ΔH), entropy (ΔS) Gibbs free-energy (ΔG) for reactions also calculated according equations. negative ΔH ΔS...
T cells mediate adaptive immunity in diverse anatomic compartments through recognition of specific antigens via unique cell receptor (TCR) structures. However, little is known about the spatial distribution an organism's TCR repertoire. Here, using high-throughput sequencing (TCRseq), we investigated repertoires sixteen tissues healthy C57B/L6 mice. We found that generally classified into three categories (lymph nodes, non-lymph node and small intestine) based on sequence similarity. Clonal...
Hepatocellular Carcinoma (HCC) is a most lethal malignancy. At first presentation, patients often already have advanced disease, and their treatment options for cure are very limited. Measurable biomarkers early diagnosis of HCC urgently needed to prolong the median over-all survival rate reduce therapeutic expenditures. Proteomics powerful analytical technique that has been widely applied search cancer biomarkers, including in HCC. High-sensitivity, high-throughput, non-invasive...