James T. Rutka
A5067215084- Glioma Diagnosis and Treatment
- Epilepsy research and treatment
- Hedgehog Signaling Pathway Studies
- Chromatin Remodeling and Cancer
- Neuroblastoma Research and Treatments
- Meningioma and schwannoma management
- Cerebrospinal fluid and hydrocephalus
- Neonatal and fetal brain pathology
- Microtubule and mitosis dynamics
- EEG and Brain-Computer Interfaces
- Pharmacological Effects and Toxicity Studies
- Epigenetics and DNA Methylation
- Fetal and Pediatric Neurological Disorders
- Neurofibromatosis and Schwannoma Cases
- Cancer, Hypoxia, and Metabolism
- Vascular Malformations Diagnosis and Treatment
- Functional Brain Connectivity Studies
- Head and Neck Surgical Oncology
- History of Medical Practice
- Advanced MRI Techniques and Applications
- Cell Adhesion Molecules Research
- Cancer-related Molecular Pathways
- Sarcoma Diagnosis and Treatment
- Spinal Dysraphism and Malformations
- Neuroscience and Neuropharmacology Research
University of Toronto
2016-2025
Hospital for Sick Children
2016-2025
SickKids Foundation
2016-2025
Neurological Surgery
1986-2023
Health Affairs
2023
Health & Medical Publishing Group
2023
Vanderbilt University Medical Center
2023
Systems, Applications & Products in Data Processing (Canada)
2023
University Health Network
2010-2022
Weatherford College
2022
Recent genomic approaches have suggested the existence of multiple distinct subtypes medulloblastoma. We studied a large cohort medulloblastomas to determine how many subgroups disease exist, they differ, and extent overlap between subgroups.We determined gene expression profiles DNA copy number aberrations for 103 primary medulloblastomas. Bioinformatic tools were used class discovery medulloblastoma based on most informative genes in data set. Immunohistochemistry subgroup-specific...
To evaluate the pattern of stability and change over time across multiple domains neurocognitive function in radiated survivors posterior fossa (PF) tumors.Thirty-four children (25 males) treated for malignant PF tumors were observed with serial clinical neuropsychologic assessments. Thirty patients medulloblastoma four ependymoma. Twelve reduced-dose 21 standard-dose cranial radiation. All received an additional boost to PF. One patient was radiation only. Standardized tests administered at...
The diagnosis of medulloblastoma likely encompasses several distinct entities, with recent evidence for the existence at least four unique molecular subgroups that exhibit genetic, transcriptional, demographic, and clinical features. Assignment subgroup through routine profiling high-quality RNA on expression microarrays is impractical in setting. planning execution trials stratify by subgroup, or which are targeted to a specific requires technologies can be economically, rapidly, reliably,...
Purpose BRAF V600E is a potentially highly targetable mutation detected in subset of pediatric low-grade gliomas (PLGGs). Its biologic and clinical effect within this diverse group tumors remains unknown. Patients Methods A combined genetic institutional study patients with PLGGs long-term follow-up was performed (N = 510). Clinical treatment data mutated PLGG (n 99) were compared large international independent cohort mutated-PLGG 180). Results 69 405 (17%) across broad spectrum histologies...
Medulloblastoma comprises four distinct molecular subgroups: WNT, SHH, Group 3, and 4. Current medulloblastoma protocols stratify patients based on clinical features: patient age, metastatic stage, extent of resection, histologic variant. Stark prognostic genetic differences among the subgroups suggest that subgroup-specific biomarkers could improve prognostication.Molecular were identified from a discovery set 673 medulloblastomas 43 cities around world. Combined risk stratification models...
Abstract Medulloblastoma is the most common malignant pediatric brain tumor, and mechanisms underlying its development are poorly understood. We identified recurrent amplification of miR-17/92 polycistron proto-oncogene in 6% medulloblastomas by high-resolution single-nucleotide polymorphism genotyping arrays subsequent interphase fluorescence situ hybridization on a human medulloblastoma tissue microarray. Profiling expression 427 mature microRNAs (miRNA) series 90 primary revealed that...
Purpose: High-frequency oscillations (HFOs), termed ripples at 80–200 Hz and fast (FRs) >200/250 Hz, recorded by intracranial electroencephalography (EEG), may be a valuable surrogate marker for the localization of epileptogenic zone. We evaluated relationship resection focal brain regions containing high-rate interictal HFOs seizure-onset zone (SOZ) determined visual EEG analysis with postsurgical seizure outcome, using extraoperative monitoring in pediatric patients automated HFO...
To uncover the genetic events leading to transformation of pediatric low-grade glioma (PLGG) secondary high-grade (sHGG).We retrospectively identified patients with sHGG from a population-based cohort 886 PLGG long clinical follow-up. Exome sequencing and array CGH were performed on available samples followed by detailed analysis entire cohort. Clinical outcome data genetically distinct subgroups obtained.sHGG was observed in 2.9% PLGGs (26 patients). Patients had high frequency nonsilent...
Intravenous fluorescein sodium has been used during resection of high-grade gliomas to help the surgeon visualize tumor margins. Several studies have reported improved rates gross-total (GTR) using high doses under white light. The recent introduction a fluorescein-specific camera that allows for high-quality intraoperative imaging and use very low dose drawn new attention this fluorophore. However, ability specifically stain glioma cells is not yet well understood.The authors designed an in...
Six isolates of the human immunodeficiency virus (HIV) showed differences in their ability to productively infect glioma-derived cell lines and early-passage brain cultures. Susceptibility HIV infection correlated well with expression astrocyte marker glial fibrillary acidic protein. The CD4 molecule was expressed on some, but not all, brain-derived cells; however, no correlation observed between protein susceptibility infection. results show that can cells, particularly those origin,...
To evaluate academic and behavioral outcome in radiated survivors of posterior fossa (PF) tumors.Fifty-three patients (36 males) treated for malignant PF tumors were seen evaluation academics and/or functioning. Forty-six medulloblastoma, seven ependymoma. Fourteen with reduced-dose cranial radiation, 34 standard-dose radiation (dose was not available four patients). All received an additional boost to the PF. One patient only. Standardized achievement tests questionnaires administered at...