Michal Zápotocký
A5043812430- Glioma Diagnosis and Treatment
- Neuroblastoma Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Cancer, Hypoxia, and Metabolism
- Chromatin Remodeling and Cancer
- Brain Metastases and Treatment
- Epigenetics and DNA Methylation
- Cancer, Stress, Anesthesia, and Immune Response
- Acute Myeloid Leukemia Research
- Cancer Genomics and Diagnostics
- Meningioma and schwannoma management
- Neurofibromatosis and Schwannoma Cases
- Renal and related cancers
- Immune cells in cancer
- Radiomics and Machine Learning in Medical Imaging
- Histone Deacetylase Inhibitors Research
- Sarcoma Diagnosis and Treatment
- Acute Lymphoblastic Leukemia research
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- Protein Degradation and Inhibitors
- Hedgehog Signaling Pathway Studies
- Pancreatic and Hepatic Oncology Research
- Ocular Oncology and Treatments
- Vascular Malformations Diagnosis and Treatment
University Hospital in Motol
2012-2025
Charles University
2014-2025
Hospital for Sick Children
2016-2022
SickKids Foundation
2016-2020
University of Toronto
2016-2018
Qatar Airways (Qatar)
2018
Sidra Medical and Research Center
2018
McMaster Children's Hospital
2017
Tata Memorial Hospital
2017
Pediatric Oncology Group
2017
Purpose BRAF V600E is a potentially highly targetable mutation detected in subset of pediatric low-grade gliomas (PLGGs). Its biologic and clinical effect within this diverse group tumors remains unknown. Patients Methods A combined genetic institutional study patients with PLGGs long-term follow-up was performed (N = 510). Clinical treatment data mutated PLGG (n 99) were compared large international independent cohort mutated-PLGG 180). Results 69 405 (17%) across broad spectrum histologies...
Infant gliomas have paradoxical clinical behavior compared to those in children and adults: low-grade tumors a higher mortality rate, while high-grade better outcome. However, we little understanding of their biology therefore cannot explain this nor what constitutes optimal management. Here report comprehensive genetic analysis an international cohort clinically annotated infant gliomas, revealing 3 subgroups. Group 1 arise the cerebral hemispheres harbor alterations receptor tyrosine...
PURPOSE Children with pediatric gliomas harboring a BRAF V600E mutation have poor outcomes current chemoradiotherapy strategies. Our aim was to study the role of targeted inhibition in these tumors. PATIENTS AND METHODS We collected clinical, imaging, molecular, and outcome information from patients V600E–mutated glioma treated across 29 centers multiple countries. RESULTS Sixty-seven were (pediatric low-grade [PLGGs], n = 56; high-grade [PHGGs], 11) for up 5.6 years. Objective responses...
Abstract Background Malignant astrocytic gliomas in children show a remarkable biological and clinical diversity. Small in-frame insertions or missense mutations the epidermal growth factor receptor gene (EGFR) have recently been identified distinct subset of pediatric-type bithalamic with unique DNA methylation pattern. Methods Here, we investigated an epigenetically homogeneous cohort malignant (n = 58) from other subtypes enriched for pediatric cases thalamic location, comparison this...
This retrospective cohort study aimed to determine the predictive relevance of clinical characteristics, additional cytogenetic aberrations, and cKIT RAS mutations, as well evaluate whether specific treatment elements were associated with outcomes in pediatric t(8;21)-positive patients acute myeloid leukemia (AML).Karyotypes 916 t(8;21)-AML reviewed for presence 228 samples screened mutations. Multivariable regression models used assess anthracyclines, cytarabine, etoposide during induction...
Abstract Objective Clinical trials have failed to demonstrate a survival benefit of adjuvant chemotherapy in diffuse intrinsic pontine gliomas (DIPG). Radiation therapy (RT) is the only effective treatment thus far and reirradiation (rRT) has become an option at time progression. The aim this study was review Canadian experience DIPG rRT with focus on safety possible efficacy approach. Method We retrospectively reviewed demographic, clinical, RT data patients treated Canada rRT. Results...
Abstract Infants with low‐grade glioma (LGG) have a poor survival. BRAFV600E mutation has been identified in pediatric LGG; however, the use of BRAF inhibitors infants never reported. A 2‐month‐old V600E mutant hypothalamic/chiasmatic progressed on chemotherapy resulting profound visual loss, massive ascites, and diencephalic syndrome. Initiation dabrafenib resulted rapid sustained disappearance clinical symptoms cytoreduction. inhibition was safely tolerated dramatic clinicoradiological...
Abstract Ependymomas encompass a heterogeneous group of central nervous system (CNS) neoplasms that occur along the entire neuroaxis. In recent years, extensive (epi-)genomic profiling efforts have identified several molecular groups ependymoma are characterized by distinct alterations and/or patterns. Based on unsupervised visualization large cohort genome-wide DNA methylation data, we highly pediatric-type tumors ( n = 40) forming cluster separate from all established CNS tumor types,...
Large-scale molecular profiling studies in recent years have shown that central nervous system (CNS) tumors display a much greater heterogeneity terms of molecularly distinct entities, cellular origins and genetic drivers than anticipated from histological assessment. DNA methylation has emerged as useful tool for robust tumor classification, providing new insights into these heterogeneous classes. This is particularly true rare CNS with broad morphological spectrum, which are not possible...
Abstract Immune checkpoint inhibition (ICI) is effective for replication-repair-deficient, high-grade gliomas (RRD-HGG). The clinical/biological impact of immune-directed approaches after failing ICI monotherapy unknown. We performed an international study on 75 patients treated with anti–PD-1; 20 are progression free (median follow-up, 3.7 years). After second progression/recurrence (n = 55), continuing ICI-based salvage prolonged survival to 11.6 months 38; P < 0.001), particularly...
OBJECTIVE Metastatic dissemination is a major treatment challenge and cause of death in patients with medulloblastoma. However, the influence molecular biology on pattern metastatic at diagnosis not known. In this study, authors sought to define location, pattern, imaging characteristics medulloblastoma metastases across subgroups diagnosis. METHODS A consecutive cohort The Hospital for Sick Children University Motol, who underwent up-front MRI craniospinal axis, was assembled allocated...
Previous studies identified recurrent fusion and duplication events in pediatric low-grade glioma (pLGG). In addition to their role diagnosis, the presence of these aid dictating therapy predicting patient survival. Clinically, BRAF alterations are most commonly using fluorescent situ hybridization (FISH). However, this method is costly, labor-intensive does not identify nonBRAF events. Here, we evaluated NanoString nCounter gene expression system for detecting 32 reported fusion/duplication...
Abstract Background The international, multicenter registry LOGGIC Core BioClinical Data Bank aims to enhance the understanding of tumor biology in pediatric low-grade glioma (pLGG) and provide clinical molecular data support treatment decisions interventional trial participation. Hence, question arises whether implementation RNA sequencing (RNA-Seq) using fresh frozen (FrFr) tissue addition gene panel DNA methylation analysis improves diagnostic accuracy provides additional benefit. Methods...