A5007268439- Transplantation: Methods and Outcomes
- Organ Transplantation Techniques and Outcomes
- Renal Transplantation Outcomes and Treatments
- Mechanical Circulatory Support Devices
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neonatal Respiratory Health Research
- Polyomavirus and related diseases
- Tracheal and airway disorders
- Pulmonary Hypertension Research and Treatments
- Organ and Tissue Transplantation Research
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Pneumocystis jirovecii pneumonia detection and treatment
- Respiratory viral infections research
- Immune cells in cancer
- Congenital Diaphragmatic Hernia Studies
- Organ Donation and Transplantation
- Immune Cell Function and Interaction
- Respiratory Support and Mechanisms
- Cardiac Ischemia and Reperfusion
- Cancer-related molecular mechanisms research
- Tuberous Sclerosis Complex Research
- Medical Imaging and Pathology Studies
University of Pennsylvania
2016-2025
Hospital of the University of Pennsylvania
2013-2025
Children's Hospital of Philadelphia
2025
Cardiovascular Institute of the South
2025
Presidio Ospedaliero
2025
Emory University
2024
California University of Pennsylvania
2024
Penn Center for AIDS Research
2024
Pennsylvania Hospital
2024
Pulmonary and Allergy Associates
2024
Drug delivery by nanocarriers (NCs) has long been stymied dominant liver uptake and limited target organ deposition, even when NCs are targeted using affinity moieties. Here we report a universal solution: red blood cell (RBC)-hitchhiking (RH), in which adsorbed onto the RBCs transfer from to first downstream of intravascular injection. RH improves for wide range viral vectors. For example, injected intravenously increases liposome organ, lungs, ~40-fold compared with free NCs. Intra-carotid...
PD-L1 + tumor cells, but not macrophages, are likely responsible for inhibiting tumor-specific T cells in early-stage human lung tumors.
Few studies have examined the lung virome in health and disease. Outcomes of transplantation are known to be influenced by several recognized respiratory viruses, but global understanding transplanted is incomplete. To define DNA within tract following we carried out metagenomic analysis allograft bronchoalveolar lavage (BAL), compared with healthy HIV+ subjects. Viral concentrates were purified from BAL analyzed shotgun sequencing. All samples contained reads mapping anelloviruses, high...
Disruption of pulmonary vascular homeostasis is a central feature viral pneumonia, wherein endothelial cell (EC) death and subsequent angiogenic responses are critical determinants the outcome severe lung injury. A more granular understanding fundamental mechanisms driving reconstitution endothelium necessary to facilitate therapeutic repair. Here, we demonstrated that TGF-β signaling through TGF-βR2 (transforming growth factor–β receptor 2) activated in ECs upon influenza infection, mice...
Primary graft dysfunction (PGD) is a principal cause of early morbidity and mortality after lung transplantation, but its pathogenic mechanisms are not fully clarified. To date, studies using standard clinical assays have linked microbial factors to PGD. We previously used comprehensive metagenomic methods characterize viruses in allografts >1 mo transplant found that levels Anellovirus, mainly torque teno (TTVs), were significantly higher than nontransplanted healthy controls. quantitative...
Cancer progression is marked by dysfunctional tumor-infiltrating lymphocytes (TIL) with high inhibitory receptor (IR) expression. Because IR blockade has led to clinical responses in some patients non-small cell lung cancer (NSCLC), we investigated how IRs influenced CD8
A central feature of progressive vascular remodeling is altered smooth muscle cell (SMC) homeostasis; however, the understanding how different populations contribute to this process limited. Here, we utilized single-cell RNA sequencing provide insight into cellular composition changes within isolated pulmonary arteries (PAs) from arterial hypertension and donor lungs. Our results revealed that skewed balanced communication network between immune structural cells, in particular SMCs....
A long-standing goal of nanomedicine is to improve a drug's benefit by loading it into nanocarrier that homes solely specific target cell and organ. Unfortunately, nanocarriers usually end up with only small percentage the injected dose (% ID) in organ, due largely clearance liver spleen. Further, cell-type-specific targeting rarely achieved without reducing organ accumulation. To solve these problems, we introduce DART (dual affinity RBCs cells), which are conjugated two ligands, one...
ObjectiveDonation after circulatory death (DCD) donors offer the ability to expand lung donor pool and ex vivo perfusion (EVLP) further contributes this by allowing for additional evaluation resuscitation of these extended criteria donors. We sought determine outcomes recipients receiving organs from DCD EVLP in a multicenter setting.MethodsThis was an unplanned post hoc analysis multicenter, prospective, nonrandomized trial that took place during 2011 2017 with 3 years follow-up. Patients...
Nanomedicine has long pursued the goal of targeted delivery to specific organs and cell types but yet achieve this with vast majority targets. One rare example success in pursuit been 25+ years studies targeting lung endothelium using nanoparticles conjugated antibodies against endothelial surface molecules. However, here we show that such "endothelial-targeted" nanocarriers also effectively target lungs' numerous marginated neutrophils, which reside pulmonary capillaries patrol for...
The longitudinal cellular interactions that drive pulmonary fibrosis are not well understood. To investigate the disease underpinnings associated with onset and progression, we generated a scRNA-seq atlas of lungs from young aged mouse models multiple subtypes Hermansky-Pudlak syndrome (HPS), collection rare autosomal recessive diseases albinism, platelet dysfunction, fibrosis. We have identified an age-dependent increase in SAA3+ inflammatory lung fibroblasts HPS mice, including...
Introduction Primary graft dysfunction (PGD) is a significant contributor to early morbidity and mortality after lung transplantation. Increased vascular permeability in the allograft has been identified as possible mechanism leading PGD. Angiopoietin-2 serves partial antagonist Tie-2 receptor induces increased endothelial permeability. We hypothesized that elevated Ang2 levels would be associated with development of Methods performed case-control study, nested within multi-center Lung...
Non-small cell lung cancer (NSCLC) is the number one killer in United States. Despite attempted curative surgical resection, nearly 40% of patients succumb to recurrent disease. High recurrence rates may be partially explained by data suggesting that 20% NSCLC harbor synchronous disease missed during resection. In this report, we describe use a novel folate receptor-targeted near-infrared contrast agent (OTL38) improve intraoperative localization pulmonary Using optical phantoms, fluorescent...
Pulmonary hypertension from pulmonary arterial or parenchymal lung disease is associated with an increased risk for primary graft dysfunction after transplantation.We evaluated the clinical determinants of severe in and developed validated a prognostic model.We conducted retrospective cohort study patients multicenter Lung Transplant Outcomes Group at transplant listing. Severe was defined as PaO2/FiO2 ≤200 allograft infiltrates 48 72 hours transplantation. Donor, recipient, operative...
Primary graft dysfunction (PGD) is a form of acute lung injury that occurs after transplantation. The definition PGD was standardized in 2005. Since time, clinical practice has evolved, and this increasingly used as primary endpoint for trials; therefore, validation warranted.