A5043183307- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Liver Disease Diagnosis and Treatment
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Diet, Metabolism, and Disease
- Heat shock proteins research
- Adipose Tissue and Metabolism
- RNA Research and Splicing
- Lipid metabolism and biosynthesis
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Metabolism, Diabetes, and Cancer
- Molecular Biology Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- RNA regulation and disease
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- Circadian rhythm and melatonin
- Adipokines, Inflammation, and Metabolic Diseases
- Diabetes and associated disorders
- Muscle Physiology and Disorders
- Cellular transport and secretion
- Physiological and biochemical adaptations
Tsinghua University
2015-2023
Guangzhou Experimental Station
2023
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2021
Center for Life Sciences
2015-2020
Harvard University
2005-2012
University of Georgia
2002-2004
S-nitrosylation links obesity and cell stress Obesity other diseases are somehow linked to malfunction of the protein-protecting functions endoplasmic reticulum (ER). Yang et al. propose a mechanism by which associated chronic inflammation may be accumulation unfolded proteins in ER. Such would normally trigger process known as protein response (UPR). However, obese mice had increased inositol-requiring protein-1 (IRE1α), ribonuclease that regulates UPR. The modified IRE1α decreased RNAse...
New assays that measure endoplasmic reticulum (ER) function and protein folding identify a small molecule, azoramide, exerts antidiabetic effects.
Increased de novo lipogenesis is a hallmark of metabolic dysfunction-associated steatotic liver disease (MASLD) in obesity, but the macronutrient carbon source for over half hepatic fatty acid synthesis remains undetermined. Here, we discover that dietary protein, rather than carbohydrates or fat, primary nutritional risk factor MASLD humans. Consistently, ex vivo tracing studies identify amino acids as major supplier tricarboxylic (TCA) cycle and isolated mouse hepatocytes. In vivo, are...
The heat shock response (HSR) is an evolutionarily conserved molecular/biochemical reaction to thermal stress that essential the survival of eukaryotic organisms. Recessive Mutator transposon mutations at maize empty pericarp2 (emp2) locus led dramatically increased expression genes, retarded embryo development, and early-stage abortion embryogenesis. developmental timing emp2 mutant lethality was correlated with initial competence kernels invoke HSR. Cloning sequence analyses revealed gene...
Obesity-associated metabolic complications are generally considered to emerge from abnormalities in carbohydrate and lipid metabolism, whereas the status of protein metabolism is not well studied. Here, we performed comparative polysome associated transcriptional profiling analyses study dynamics functional implications endoplasmic reticulum (ER)-associated synthesis mouse liver under conditions obesity nutrient deprivation. We discovered that ER livers obese mice exhibits a general...
In this study, a universal protein expression enhancement RNA tool, termed RNAe, was developed by modifying recently discovered natural long non-coding RNA. At the moment, RNAe is only technology for gene enhancement, as opposed to silencing, at post-transcriptional level. With technology, an of 50–1000% achievable, with more than 200% achieved in most cases. This work identified sufficient and necessary element function, which found be merely 300 nucleotides named minRNAe. It contains 72-nt...
MyoD is a skeletal muscle-specifically expressed transcription factor and plays critical role in regulating myogenesis during muscle development regeneration. However, whether myofibers-expressed exerts its metabolic function whole body energy homeostasis vivo remains largely unknown. Here, we report that genetic deletion of Myod male mice enhances the oxidative metabolism and, intriguingly, renders resistant to high fat diet-induced obesity. By performing lipidomic analysis...
Nutrients stimulate the anabolic synthesis of proteins and lipids, but selective insulin resistance in obesity biases program toward lipogenesis. Here, we report identification a DNAJB9-driven that favors protein energy production over lipid accumulation. We show there are two pools DNAJB9 cochaperone. ER lumen promotes degradation lipogenic transcription factor SREBP1c through ERAD, whereas its counterpart on membrane assembly mTORC2 cytosol stimulates ATP. The expression Dnajb9 is induced...
The principles guiding the diurnal organization of biological pathways remain to be fully elucidated. Here, we perturb hepatic transcriptome through nutrient regulators (high-fat diet and mTOR signaling components) identify enduring properties pathway organization. Temporal separation counter-regulation between energy metabolism inflammation/proliferation emerge as persistent features across animal models, network analysis identifies G0s2 Rgs16 genes potential mediators at...
Abstract Both iron overload and deficiency have been reported in obesity metabolic syndromes. Due to the presence of multiple intracellular pools dynamic nature mobilization use, actual status contribution free metabolically active toward syndrome remain be established. The discovery nuclear receptor coactivator 4 (NCOA4) as a ferritinophagy provides an opening address connection between diseases. This study aims specifically dissect role hepatic lipid metabolism steatosis. We conducted...
The paralogous maize proteins EMPTY PERICARP2 (EMP2) and HEAT SHOCK FACTOR BINDING PROTEIN2 (HSBP2) each contain a single recognizable motif: the coiled-coil domain. EMP2 HSBP2 accumulate differentially during development heat stress. Previous analyses revealed that is required for regulation of shock protein (hsp) gene expression also embryo morphogenesis. Developmentally abnormal emp2 mutant embryos are aborted early embryogenesis. To analyze function postembryonic stages, plants mosaic...
Abstract Skeletal muscle is the largest metabolic and endocrine organ. It secretes various peptides that contribute to regulating body energy homeostasis by communicating with other organs. However, it unknown whether muscle-secreted lipids exert a similar function. Myod specifically expressed in skeletal muscle. Here, we report genetic deletion of mice enhanced oxidative metabolism and, intriguingly, rendered resistant HFD-induced obesity. By performing lipidomic analysis muscle-conditioned...
Most eukaryotes exhibit diurnal rhythms in their metabolism, growth, and cell cycle control to accommodate the 24-hour light-dark cycles on earth surface. Circadian rhythm is not a passive response exposure of sunlight, but driven by an internal, automated clock system, i.e. circadian clock. The comprised multiple feedback loops levels transcription, translation, post-translational modification. Just like any clock, can be reset, entrained, environmental/external factors, including light,...
Abstract Regulated ER-to-Golgi trafficking is a fundamental cellular process that enables ER-resident transcription factors to sense perturbations in the ER environment and activates transcriptional programs restore homeostasis. Current models suggest sensor activation initiated by dissociation from its binding partners. Here we challenge this model demonstrating unfolded protein ATF6 sorted Golgi as newly synthesized peripheral membrane beyond reach of retainer, translocon inhibition alone...
Abstract Increased de novo lipogenesis (DNL) is a hallmark of nonalcoholic fatty liver disease (NAFLD) in obesity, but the macronutrient source for >80% carbon backbone acid synthesis has not been determined. Here we take an integrated approach to dissect nutrient metabolism, both ex vivo and . We discover castling effect glucose glutamine metabolism through isotope tracing studies that limits entrance into glutamine-dominated tricarboxylic cycle (TCA) DNL pathways. In with high...