A5085288125- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Zebrafish Biomedical Research Applications
- Neuroendocrine regulation and behavior
- Epilepsy research and treatment
- Receptor Mechanisms and Signaling
- Sleep and Wakefulness Research
- Neurotransmitter Receptor Influence on Behavior
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Neurological disorders and treatments
- Stress Responses and Cortisol
- Neurobiology and Insect Physiology Research
- Nicotinic Acetylcholine Receptors Study
- Genetics and Neurodevelopmental Disorders
- CCD and CMOS Imaging Sensors
- Alzheimer's disease research and treatments
- Neurogenesis and neuroplasticity mechanisms
- EEG and Brain-Computer Interfaces
- Animal Behavior and Reproduction
- Functional Brain Connectivity Studies
- Neonatal and fetal brain pathology
- Attention Deficit Hyperactivity Disorder
Icahn School of Medicine at Mount Sinai
2018-2025
Allen Institute for Brain Science
2018-2025
University of California, Los Angeles
2014-2019
University of California, San Diego
2009-2013
Columbia University
2013
Abstract Tracking animal behavior by video is one of the most common tasks in life sciences. Although commercial software exists for executing this task, they often present enormous cost to researcher and can entail purchasing hardware that expensive lacks adaptability. Additionally, underlying code proprietary. Alternatively, available open-source options frequently require model training be challenging those inexperienced with programming. Here we an platform independent set analysis...
The Pavlovian conditioned freezing paradigm has become a prominent mouse and rat model of learning memory, as well pathological fear. Due to its efficiency, reproducibility, well-defined neurobiology, the widely adopted in large-scale genetic pharmacological screens. However, one major shortcoming use behavior been that it required tedious hand scoring, or variety proprietary automated methods are often poorly validated difficult obtain implement. Here we report an extensive validation Video...
Miniature microscopes have gained considerable traction for in vivo calcium imaging freely behaving animals. However, extracting signals from raw videos is a computationally complex problem and remains bottleneck many researchers utilizing single-photon imaging. Despite the existence of powerful analysis packages designed to detect extract dynamics, most either key parameters that are hard-coded or insufficient step-by-step guidance validations help users choose best parameters. This makes...
Abstract Memories are encoded in neural ensembles during learning 1–6 and stabilized by post-learning reactivation 7–17 . Integrating recent experiences into existing memories ensures that contain the most recently available information, but how brain accomplishes this critical process remains unclear. Here we show mice, a strong aversive experience drives offline ensemble of not only memory also neutral formed 2 days before, linking fear to previous memory. Fear specifically links...
Modafinil has been shown to promote wakefulness and some studies suggest the drug can improve cognitive function. Because of many similarities, mechanism action may be comparable classical psychostimulants, although exact mechanisms modafinil's actions in enhancement are unknown. The current study aims further examine effects modafinil as a enhancer on hippocampus-dependent memory mice. A high dose (75 mg/kg ip) given before training improved acquisition Morris water maze. When only testing,...
Memory difficulties are commonly associated with temporal lobe epilepsy (TLE) and cause significant disability. This article reviews the role of altered hippocampal theta oscillations theta-gamma coupling as potential causes memory disturbance in epilepsy, dissecting mechanisms underlying these changes large-scale neuronal synchronization. We discuss development treatments for cognitive dysfunction directed at restoring rhythmicity future directions research.
Sleep has been implicated as playing a critical role in memory consolidation. Emerging evidence suggests that reactivation of memories during sleep may facilitate the transfer declarative from hippocampus to neocortex. Previous rodent studies have utilized sleep-deprivation examine The present study uses novel, naturalistic paradigm effect phase on Pavlovian fear conditioning, task with both hippocampus-dependent and -independent components (contextual vs. cued memories). Mice were trained 1...
Tracking animal behavior by video is one of the most common tasks in neuroscience. Previously, we have validated ezTrack, a free, flexible, and easy-to-use software for analysis behavior. ezTrack's Location Module can be used positional an individual applicable to wide range behavioral tasks. Separately, Freeze Analysis designed defensive freezing ezTrack supports desirable tools, including options cropping masking portions field view, defining regions interest, producing summary data...
ABSTRACT Alzheimer’s disease (AD) is a chronic neurodegenerative disorder that characterized by memory loss and progressive cognitive impairments. In mouse models of AD pathology, studies have found neuronal synaptic deficits in the hippocampus, but less known about what happens medial entorhinal cortex (MEC), which primary spatial input to hippocampus an early site pathology. Here, we measured intrinsic excitability activity MEC layer II (MECII) stellate cells, MECII pyramidal III (MECIII)...
Abstract Memories are encoded in neural ensembles during learning and stabilized by post-learning reactivation. Integrating recent experiences into existing memories ensures that contain the most recently available information, but how brain accomplishes this critical process remains unknown. Here we show mice, a strong aversive experience drives offline ensemble reactivation of not only memory also neutral formed two days prior, linking fear from to previous memory. We find specifically...
Alzheimer's disease (AD) is a chronic neurodegenerative disorder characterized by memory loss and progressive cognitive impairments. In mouse models of AD pathology, studies have found neuronal synaptic deficits in hippocampus, but less known about changes medial entorhinal cortex (MEC), which the primary spatial input to hippocampus an early site pathology. Here, we measured intrinsic excitability activity MEC layer II (MECII) stellate cells, MECII pyramidal III (MECIII) excitatory neurons...
Alzheimer's disease (AD) is a neurodegenerative characterized by progressive impairments in episodic and spatial memory, as well circuit network-level dysfunction. While functional medial entorhinal cortex (MEC) hippocampus (HPC) have been observed patients rodent models of AD, it remains unclear how communication between these regions breaks down disease, what specific physiological changes are associated with the onset memory impairment. We used silicon probes to simultaneously record...
Abstract Memory-updating is critical in dynamic environments because updating memories with new information promotes versatility. However, little known about how are updated information. To study neuronal ensembles might support memory-updating, we used a hippocampus-dependent spatial reversal task to measure hippocampal ensemble dynamics when mice switched navigational goals. Using Miniscope calcium imaging, identified (co-active neurons) dorsal CA1 that were spatially tuned and stable...
Abstract Miniaturized fluorescence microscopes (miniscopes) enable imaging of calcium events from a large population neurons in freely behaving animals. Traditionally, miniscopes have only been able to record single wavelength. Here, we present new open-source dual-channel Miniscope that simultaneously records two wavelengths To simultaneous acquisition fluorescent wavelengths, incorporated CMOS sensors into Miniscope. validate our Miniscope, imaged hippocampal CA1 region co-expressed...