A5102860836- interferon and immune responses
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Ubiquitin and proteasome pathways
- Immune cells in cancer
- Inflammasome and immune disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Viral Infections and Vectors
- Epigenetics and DNA Methylation
- Heat shock proteins research
- Immunotherapy and Immune Responses
- Cytokine Signaling Pathways and Interactions
- RNA regulation and disease
- Cancer-related Molecular Pathways
- Phytochemical Studies and Bioactivities
- Natural product bioactivities and synthesis
- Flavonoids in Medical Research
- Histone Deacetylase Inhibitors Research
- Melanoma and MAPK Pathways
- Cancer-related molecular mechanisms research
- Macrophage Migration Inhibitory Factor
Ningbo University
2024
Second Military Medical University
2009-2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2020
Institute of Immunology
2016
Zhejiang University
2003-2015
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2015
Academy of Medical Sciences
2015
Institute of Physiology and Basic Medicine
2015
Zhejiang Academy of Medical Sciences
2008
Abstract Extracellular heat shock proteins (HSP) can activate dendritic cells (DC) and monocytes/macrophages, HSP derived from tumor have been regarded as potent adjuvant facilitating presentation of Ags induction antitumor immunity. However, the roles underlying mechanisms releasable in immunity not fully elucidated. In this study, we report that stress induce release various cells, which, turn, to produce chemokines for chemoattraction DC T via TLR4 signaling pathway. vivo, find...
Exosomes derived from dendritic cells or tumor are a population of nanometer-sized membrane vesicles that can induce specific antitumor immunity. During investigation the effects hyperthermia on immune response, we found exosomes heat-stressed (HS-TEX) could chemoattract and activate (DC) T more potently than by conventional tumor-derived exosomes. We show HS-TEX contain chemokines, such as CCL2, CCL3, CCL4, CCL5, CCL20, chemokine-containing functionally competent in chemoattracting CD11c(+)...
Promoting apoptosis is a strategy for cancer drug discovery. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces in wide range of malignant cells. However, several cancers, including human hepatocellular carcinoma (HCC), exhibit major resistance to TRAIL-induced cell death. Melittin, water-soluble 26-amino acid peptide derived from bee venom Apis mellifera, can exert toxic or inhibitory effects on many types tumor Here we report that melittin induce HCC cells by...
Abstract Ubiquitination is one of the most prevalent protein posttranslational modifications. Here, we show that E3 ligase Nedd4l positively regulates antiviral immunity by catalyzing K29-linked cysteine ubiquitination TRAF3. Deficiency significantly impairs type I interferon and proinflammatory cytokine production induced virus infection both in vitro vivo. deficiency inhibits virus-induced TRAF3, binding between TRAF3 TBK1, subsequent phosphorylation TBK1 IRF3. directly interacts with...
The carboxyl terminus of constitutive heat shock cognate 70 (HSC70)-interacting protein (CHIP, also known as Stub1) is a U box-containing E3 ubiquitin ligase that important for quality control. role CHIP in innate immunity not known. Here, we report knockdown inhibits Toll-like receptor (TLR) 4- and TLR9-driven signaling, but TLR3-driven signaling; proinflammatory cytokine type 1 interferon (IFN) production; maturation antigen-presenting cells, including macrophages dendritic cells. We...
Protein ubiquitination regulated by ubiquitin ligases plays important roles in innate immunity. However, key regulators of during response and new types (apart from Lys48- Lys63-linkage) control signaling have not been clearly understood. Here we report that F-box only protein Fbxo21, a functionally unknown component SCF (Skp1–Cul1–F-box protein) complex, facilitates Lys29-linkage activation ASK1 (apoptosis signal-regulating kinase 1), promotes type I interferon production upon viral...
The endoplasmic reticulum (ER)–localized stimulator of interferon genes (STING) is the core adaptor for pathogenic-DNA–triggered innate response. Aberrant activation STING causes autoinflammatory and autoimmune diseases, raising concern about how finely tuned during response to pathogenic DNAs. Here, we report that transmembrane domain (TM)–containing ER-localized E3 ubiquitin ligase TRIM13 (tripartite motif containing 13) required restraining inflammatory deficiency enhances cytokine...
Abstract Retinoic acid–inducible gene I (RIG-I) signaling is critical to host innate immune response against RNA virus infection. Numerous factors use different mechanisms regulate RIG-I signaling. In this study, we report that STAT family member STAT4 promotes RIG-I–triggered type IFN production in antiviral immunity. Silencing of impaired IFN-β macrophages upon infection, whereas overexpression enhanced RIG-I–induced promoter activation and IFN-stimulated element activity. increased...
Various pattern recognition receptors (PRRs) are activated in response to viral infection stimulate the production of type I interferons (IFNs). However, central responses all these is their activation kinase TBK1, which stimulates transcription by IFN regulatory factor 3 (IRF3). We investigated mechanism activity TBK1 stimulated infection. found that tyrosine Src promoted phosphorylation on Tyr179 upon RAW264.7 macrophages. Mutation alanine resulted impaired autophosphorylation at Ser172,...
Host immune cells can detect and destruct invading pathogens via pattern-recognition receptors. Small Rap GTPases act as conserved molecular switches coupling extracellular signals to various cellular responses, but their roles regulators in Toll-like receptor (TLR) signalling have not been fully elucidated. Here we report that Ras guanine nucleotide-releasing protein 3 (RasGRP3), a nucleotide-exchange factor activating Rap1, limits production of proinflammatory cytokines (especially IL-6)...
Signal transducer and activator of transcription 3 (STAT3) is one the key players in liver cancer. Increased levels phosphorylated STAT3 (p-STAT3) have been detected many cancers including hepatocellular carcinoma (HCC), are usually associated with a more aggressive phenotype poor prognosis. In addition to aberrant activation STAT3, upregulation total was also HCC, for which underlying mechanisms significance remain be fully elucidated. Here we report that reciprocal regulation exists...
Sensing of pathogenic nucleic acids by pattern recognition receptors (PRR) not only initiates anti-microbe defense but causes inflammatory and autoimmune diseases. E3 ubiquitin ligase(s) critical in innate response need to be further identified. Here we report that the tripartite motif-containing ligase TRIM41 is required antiviral through facilitating acids-triggered signaling pathway. deficiency impairs production cytokines type I interferons macrophages after transfection with acid-mimics...
Proper activation of Toll-like receptor (TLR)-mediated signaling and production proinflammatory cytokines are critical for the initiation innate immunity, while specific mechanism maintaining inflammatory homeostasis remains mostly unknown. Here, we show that Ets2 is upregulated following LPS VSV stimulation. knockdown or knockout leads to increased IL-6, TNF-α, IFN-β in macrophages. Consistently, Ets2-deficient mice exacerbated cytokine more susceptible CLP-induced sepsis. Mechanistically,...
Uncontrolled viral replication and excessive inflammation are the main causes of death in host infected with virus. Hence inhibition intracellular production innate cytokines, which key strategies hosts to fight virus infections, need be finely tuned eliminate viruses while avoid harmful inflammation. The E3 ligases regulating subsequent cytokines remain fully characterized. Here we report that deficiency ubiquitin-protein ligase HECTD3 results accelerated RNA clearance reduced inflammatory...
TLRs are critical innate immune sensors in the induction of proinflammatory cytokines to eliminate invading pathogens. However, mechanisms for full activation TLR-triggered response need be fully understood. The murine CMRF-35-like molecule (CLM)-3 is a representative CLM family belonging Ig superfamily. Considering that CLM-3 selectively expressed macrophages and roles members remain unclear, this study we investigated role regulation response. We found was an endosome/lysosome-localized...