A5101433797- RNA modifications and cancer
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- HVDC Systems and Fault Protection
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Biomedical Ethics and Regulation
- Microbial metabolism and enzyme function
- 3D Printing in Biomedical Research
- Arsenic contamination and mitigation
- Cardiac Ischemia and Reperfusion
- Mitochondrial Function and Pathology
- Cardiac electrophysiology and arrhythmias
- Retinoids in leukemia and cellular processes
- MicroRNA in disease regulation
- Heat shock proteins research
- Cardiac Structural Anomalies and Repair
- Circular RNAs in diseases
- RNA Research and Splicing
- Cardiac Fibrosis and Remodeling
- Ion channel regulation and function
- Tissue Engineering and Regenerative Medicine
- Traditional Chinese Medicine Analysis
- Ginseng Biological Effects and Applications
Second Affiliated Hospital of Harbin Medical University
2021-2024
Harbin Medical University
2011-2024
Tianjin University of Traditional Chinese Medicine
2024
Guangdong Academy of Medical Sciences
2023
Guangdong General Hospital
2023
Guangdong Provincial People's Hospital
2023
Second Affiliated Hospital of Zhejiang University
2023
Guiyang Medical University
2022
Affiliated Hospital of Guizhou Medical University
2022
Sun Yat-sen University
2019-2022
N6-methyladenosine (m6A) is the most abundant internal modification in mammalian mRNAs. Despite its functional importance various physiological events, role of m6A chemical carcinogenesis remains largely unknown. Here we profiled dynamic mRNA during cellular transformation induced by carcinogens and identified a subset cell transformation-related, concordantly modulated sites. Notably, increased 3'-UTR oncogene CDCP1 was found malignant transformed cells. Mechanistically, methyltransferase...
N6-methyladenosine (m6A) RNA modification, a dynamic and reversible process, is essential for tissue development pathogenesis. However, the potential involvement of m6A in regulation cardiomyocyte (CM) proliferation cardiac regeneration remains unclear. In this study, we aimed to investigate role modification heart during postnatal adult injury. Methods results: identified downregulation demethylase ALKBH5, an "eraser" that responsible increased methylation, after birth. Notably, ALKBH5...
Abstract In this study, we identified that a conserved circular RNA (circRNA) DICAR, which was downregulated in diabetic mouse hearts. DICAR had an inhibitory effect on cardiomyopathy (DCM), as the spontaneous cardiac dysfunction, cell hypertrophy, and fibrosis occurred deficiency ( +/− ) mice, whereas DCM alleviated DICAR-overexpressed Tg mice. At cellular level, found overexpression of inhibited, but knockdown enhanced cardiomyocyte pyroptosis. molecular DICAR-VCP-Med12 degradation could...
Methyltransferase-like 3 (METTL3) is the main enzyme for N6-methyladenosine (m6A)-based methylation of RNAs and it has been implicated in many biological pathophysiological processes. In this study, we aimed to explore potential involvement METTL3 osteoblast differentiation decipher underlying cellular molecular mechanisms. We demonstrated that downregulated human osteoporosis ovariectomized (OVX) mouse model, as well during osteogenic differentiation. Silence by short interfering RNA...
Pathological cardiac hypertrophy induced by adrenergic overactivation can subsequently develop to heart failure which remains as a leading cause of mortality worldwide.Tanshinone IIA is lipid-soluble pharmacologically active compound extracted from the rhizome Chinese herb Salvia miltiorrhiza, well-known traditional medicine used for treatment cardiovascular disorders.However, little know about effect Tanshinone on hypertrophy.The present study was aimed investigate whether prevents...
Aims: Calcium-sensing receptor (CaR) acts as a G protein coupled that mediates the increase of intracellular Ca2+ concentration. The expression CaR has been confirmed in various cell types, including cardiomyocytes, smooth muscle cells, neurons and vascular endothelial cells. However, whether is expressed functions cardiac fibroblasts remained unknown. present study investigated played role fibroblast proliferation extracellular matrix (ECM) secretion, both cultured rat neonatal model...
The discovery of reprogramming and generation human-induced pluripotent stem cells (iPSCs) has revolutionized the field regenerative medicine opened new opportunities in cell replacement therapies. While iPSCs represents a significant breakthrough, clinical relevance for cell-based therapies requires high-quality specialized through robust reproducible directed differentiation protocols. We have recently reported manufacturing human iPSC master banks (MCB) under current good practices...
N6-methyladenosine (m6A), as the most abundant modification of mammalian messenger RNAs, is essential for tissue development and pathogenesis. However, biological significance m6A methylation in cardiac differentiation remains largely unknown. Here, we identify that downregulation demethylase ALKBH5 responsible increase cardiomyocyte fate determination human embryonic stem cells (hESCs) from mesoderm (MESs). In contrast, overexpression remarkably blocks hESCs. Mechanistically, KDM5B RBBP5,...
The clinical effectiveness of human induced pluripotent stem cells (iPSCs) is highly dependent on a few key quality characteristics including the generation high cell bank, long-term genomic stability, post-thaw viability, plating efficiency, retention pluripotency, directed differentiation, purity, potency, and sterility. We have already reported establishment iPSC master banks (MCBs) working (WCBs) under current good manufacturing procedure (cGMP)-compliant conditions. In this study, we...
Monocyte-to-M0/M1 macrophage differentiation with unclear molecular mechanisms is a pivotal cellular event in many cardiovascular diseases including atherosclerosis. Long non-coding RNAs (lncRNAs) are group of protein expression regulators; however, the roles monocyte-lncRNAs and its related vascular still unclear. The study aims to investigate whether novel leukocyte-specific lncRNA Morrbid could regulate atherogenesis. We identified that was increased monocytes arterial walls from...
<b><i>Objective:</i></b> The aim of our study was to assess the effects altered salt and potassium intake on urinary renalase serum dopamine levels in humans. <b><i>Methods:</i></b> Forty-two subjects (28-65 years age) were selected from a rural community of<b> </b>northern China. All sequentially maintained low-salt diet for 7 days (3.0 g/day NaCl), high-salt an additional (18.0 with supplementation final NaCl + 4.5 KCl)....
This study aimed to investigate the association of renalase with blood pressure (BP) and brachial-ankle pulse wave velocity (baPWV) in order better understand role pathogenesis hypertension atherosclerosis.A total 344 subjects normal kidney function were recruited from our previously established cohort Shaanxi Province, China. They divided into normotensive (NT) hypertensive (HT) groups or high baPWV on basis BP levels measured an automatic waveform analyzer. Plasma was determined through...
Chronic exposure to inorganic arsenic is a major environmental public health issue worldwide affecting more than 220 million of people. Previous studies have shown the correlation between poisoning and cellular senescence; however, knowledge regarding mechanism effective prevention measures has not been fully studied. First, associations among ERK/CEBPB signaling pathway, oxidative stress, arsenic-induced skin cell senescence were confirmed using HaCaT model. In arsenic-exposed group,...
This study aimed to investigate the effect of N6-methyladenosine (m6 A) modification in modulating inflammatory homeostasis arsenic (As)-induced skin lesions. Our bioinformatic analysis revealed abnormal expression m6 A RNA methylation regulators and cytokines arsenic-exposed population. In human keratinocytes, arsenite increased levels by upregulating methyltransferase like 3 (METTL3), mediating disordered secretion indicators that reflect (IL-6, IL-17, IL-10). The reflecting...
By a cDNA array representing 2308 signal transduction-related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations four genes were confirmed real-time quantitative RTPCR; and aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transiently Huh-7, human hepatoma cell line. Of 2,308 examined, 34 up-regulated 56 down-regulated. These 90 involved oncogenes, tumor...
Down syndrome (DS) is a common genetic condition in which person born with an extra copy of chromosome 21. Intellectual disability the most characteristic DS. N6-methyladenosine (m6A) RNA modification that implicated many biological processes. It highly enriched within brain and plays essential role human development. However, mRNA m6A fetal DS has not been explored. Here, we report profiles expression cortex tissue from DSs controls. We observed tissues was reduced genome-wide, may be due...